A seroepidemiological study on bluetongue virus in dairy cattle in the central valley of California

A seroepidemiological study on bluetongue virus (BTV) infection in California dairy cattle was conducted to estimate the prevalence and distribution by age and season of BTV group-reactive antibodies and to look for possible associations between the presence of antibodies and cattle age or breed and farm. Between December 1985 and March 1987, a sample of cattle was tested at approximately two-month intervals for BTV group-reactive antibodies using an enzyme-linked immunosorbent assay (ELISA). Data taken during the month of December 1986 were used to evaluate possible associations between a positive antibody test and certain intrinsic (age, breed) and extrinsic (farm) factors.Univariate and multivariate statistical analyses using the -square test for associations and multiple logistic regression, respectively, were carried out for possible associations between positive antibody tests to BTV and each factor of interest. The strengths of the associations were determined using estimates of the odds ratio.Of the 3774 serum samples tested, 238 (6.3%) were from calves, 1045 (27.6%) were from heifers and 2492 (66.0%) were from cows. Seroprevalence varied from nil in calves on two occasions to over 90% on several occasions in cows. Cows consistently had higher prevalence rates than heifers or calves across all test dates (p<0.05). The seroprevalence of BTV group-reactive antibodies also showed a seasonal fluctuation, with the highest rates occurring during the warmer months of the year. These highest prevalence rates coincided with heavy activity of the known vector of BTV, Culicoides spp. Breed and farm effects were not statistically significant (p>0.05). With the exception of one farm, all cattle were of the Holstein breed, which reduced confidence in assessing any breed effect in this study. Relative estimates of the sensitivity and specificity of BTV ELISA were 87% and 100% respectively, compared to the standard agar gel immunodiffusion (AGID) test.The observations support previous findings of seasonal distribution of BTV antibodies and suggest an age relationship, whereby older cattle are more likely to be positive to BTV group-reactive antibodies than younger cattle.     

Q fever (Coxiella burnetii) investigations in dairy cattle: persistence of antibodies after vaccination.

The immune response and persistence of antibodies were investigated in dairy cattle vaccinated with formalin-inactivated phase (ph) I Coxiella burnetii vaccine agglutinating antibody geometric mean titer (GMT) of 193.2 at 1 month after vaccination compared to a GMT of 2.0 for nonvaccinated calves. The agglutinating antibodies gradually decreased in vaccinated cattle, but the GMT remained approximately 4 times higher than that for the nonvaccinated group for at least 20 months. Results of serotests at 2 months after revaccination indicated a rapid increase in the GMT to 177.0 with agglutinating titers between 1:64 and 1:512.     

Drivers,challenges and opportunities of forage technology adoption by smallholder cattle households in Cambodia

Forage technology has been successfully introduced into smallholder cattle systems in Cambodia as an alternative feed source to the traditional rice straw and native pastures, improving animal nutrition and reducing labour requirements of feeding cattle. Previous research has highlighted the positive impacts of forage technology including improved growth rates of cattle and household time savings. However, further research is required to understand the drivers, challenges and opportunities of forage technology for smallholder cattle households in Cambodia to facilitate widespread adoption and identify areas for further improvement. A survey of forage-growing households (n = 40) in July–September 2016 examined forage technology adoption experiences, including reasons for forage establishment, use of inputs and labour requirements of forage plot maintenance and use of forages (feeding, fattening, sale of grass or seedlings and silage). Time savings was reported as the main driver of forage adoption with household members spending approximately 1 h per day maintaining forages and feeding it to cattle. Water availability was reported as the main challenge to this activity. A small number of households also reported lack of labour, lack of fencing, competition from natural grasses, cost of irrigation and lack of experience as challenges to forage growing. Cattle fattening and sale of cut forage grass and seedlings was not found to be a widespread activity by interviewed households, with 25 and 10% of households reporting use of forages for these activities, respectively. Currently, opportunities exist for these households to better utilise forages through expansion of forage plots and cattle activities, although assistance is required to support these households in addressing current constraints, particularly availability of water, if the sustainability of this feed technology for smallholder cattle household is to be established in Cambodia.     

Application of a linear programming model to the control of African trypanosomiasis

A linear programming (LP) model was designed to evaluate trypanosomiasis control activities in south-western Ethiopia. The objectives included maximizing net benefits, utilization of unskilled labor, and resettlement of reclaimed land, and decreasing the prevalence of trypanosomiasis from 20% to less than 5% at the end of 5 years, subject to epidemiological, ecological and economic constraints. The model was a multiperiod specification with 127 equations and 81 activities for the project period of 5 years. The optimal solution required reclamation of 5221 km² of tsetse infested land and used treatment of cattle at maximal levels. At the end of the project period, the prevalance decreased to 2% with net benefits of E$1.281 million. LP provided a potentially optimal means of resource allocation in the short run and a means of identifying those restrictive resources which could be vital to long range planning.     

南极半岛海洋气候区的土壤Ⅲ.冻-融作用与水分状况

自由水活动在南极海洋性气候区土壤形成与演化过程中发挥极为重要的作用。本文对土壤自由水的来源、冻-融作用、永冻层和活动层动态以及自由水参与下的物质迁移过程等几个方面进行了论述,讨论了上述不同过程的影响因素以及土壤发生学意义,指出以土壤水形态转化和自由水活动为基础的土壤过程具有显著的微域性特点,是决定本区土壤发生类型与分布模式的重要因素之一。     

Circadian system controlling release of sperm in the insect testes

Release of mature sperm from the testis into seminal ducts of the gypsy moth exhibits a circadian rhythm. The rhythm of sperm release was shown to persist in vitro, in isolated complexes of testis and seminal ducts cultured in light-dark cycles or in constant darkness. The phase of the rhythm was also reset in vitro by exposure to shifted light-dark cycles. Therefore, the testis-seminal ducts complex from the gypsy moth is photosensitive and contains a circadian pacemaker, which controls the rhythm of sperm movement. This finding extends the range of structures in multicellular organisms that are known to contain circadian oscillators and provides a new model system in which circadian mechanisms may be studied.     

"Tubules-Within-a-Tubule" Hierarchical Order of Mesoporous Molecular Sieves in MCM-41

The recently discovered mesoporous aluminosilicate MCM-41 consists of hexagonal arrays of nanometer-sized cylindrical pores. It is shown that this material can be synthesized by cooperative condensation of silicate and cylindrical cationic micelles. Careful control of the surfactant-water content and the rate of condensation of silica at high alkalinity resulted in hollow tubules 0.3 to 3 micrometers in diameter. The wall of the tubules consisted of coaxial cylindrical pores, nanometers in size, that are characteristic of those of MCM-41. The formation of this higher order structure may take place through a liquid crystal phase transformation mechanism involving an anisotropic membrane-to-tubule phase change. The hierarchical organization of this "tubules-within-a-tubule" particle texture is similar to that of the frustules of marine diatoms.     

An experimental approach to the photolysis of pesticides adsorbed on soil: Thidiazuron

An experimental procedure has been developed for conducting photolysis studies with pesticides adsorbed by soil surfaces. In these experiments, solutions of thidiazuron were sprayed on segments of a 0.5-mm layer of soil covering a glass plate. The soil surface was irradiated using simulated sunlight. The light source was a xenon arc in combination with glass filters that absorbed light of wavelength <290 nm. During irradiation, one half of the sprayed areas was covered to obtain nonirradiated samples. After various time intervals, groups of three irradiated and three non-irradiated segments of the soil were scraped off and extracted; the extracts were analysed. Information was obtained on the photoproducts formed and on the kinetics of photodegradation of thidiazuron.