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排序方式: 共有215条查询结果,搜索用时 15 毫秒
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Yoshimichi GODA Shinya MIZUTANI Yuko MIZUTANI Go KITAHARA Riki SISWANDI Kenta WAKABAYASHI Shidow TORISU Yasuyuki KANEKO Yuichi HIDAKA Takeshi OSAWA Taketoshi ASANUMA Reiichiro SATO Hiroyuki SATOH 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(10):1430
Cryptorchidism is defined as the failure of the testis to descend into the scrotal position. Bulls with cryptorchidism have problems in both meat quality and husbandry management; thus, it is greatly important to accurately identify the retained testis and remove it during the early stage. Abdominal computed tomography (CT) was performed under general anesthesia in 34 bulls aged 3–9 months with cryptorchidism. All bulls underwent laparoscopic or incision approach for cryptorchidectomy, and 40 testes were dissected. The detection rates of retained testes were 64.5% in the abdominal cavity and 100% in the subcutaneous inguinal region, and the total detection rate was 72.5%. Furthermore, all cases in this study were suspected to have intra-abdominal cryptorchidism in primary care, but CT revealed that 22.5% of cases had cryptorchidism in the subcutaneous inguinal region. The CT value (mean ± standard deviation) of the retained testes was 20.96 ± 7.54 Hounsfield Unit, and the CT value and size of the retained testes showed a positive weak correlation with bovine age. Therefore, there is the demerit that general anesthesia and a huge device are necessary; nevertheless, CT is suggested to be useful in identifying the location of retained testes and selecting an appropriate surgical approach in bulls with cryptorchidism. Moreover, CT findings suggested that the maturation of the retained testes might depend not on the descending process but on age. 相似文献
3.
Yukino TAMAMURA-ANDOH Nobuyuki TANAKA Keisuke SATO Yoshino MIZUNO Nobuo ARAI Ayako WATANABE-YANAI Masato AKIBA Masahiro KUSUMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(5):754
We examined the antimicrobial susceptibility of 848 Escherichia coli isolates from 237 feces samples of wild sika deer (Cervus nippon) captured between 2016 and 2019 in 39 of the 47 prefectures of Japan. Five of the 237 wild sika deer (2.1%) carried E. coli with resistance to at least one antimicrobial, and all the resistant isolates showed resistance to tetracycline. The resistant isolates contained antimicrobial resistance genes that were similar to those in E. coli derived from humans and farm animals. Although wild sika deer are not currently likely to be a source for the transmission of antimicrobial resistance in Japan, they can potentially mediate antimicrobial resistance spread by coming into contact with humans, animals, and their surroundings. 相似文献
4.
Atsushi KIMURA Yo-Han KIM Kazuyoshi HASHIZUME Akira ITO Katsuyuki MUKAI Keiichiro KIZAKI Shigeru SATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(5):829
We investigated the effect of oral administration of β-cryptoxanthin (β-CRX) on its serum concentration and peripheral neutrophil functions by the chemiluminescence (CL) response in Holstein cattle. A single oral administration of β-CRX was performed for serum β-CRX concentration (0, 0.05, 0.1, or 0.2 mg/kg body weight [BW]) and for peak CL response of peripheral neutrophils (0.2 mg/kg BW). The serum β-CRX concentration was peaked on 2 days after, similar to peak CL response on 3 days after β-CRX administration. Therefore, a single oral administration of β-CRX (0.2 mg/kg BW) induces higher serum concentration and concurrently enhances bactericidal ability of peripheral neutrophils in Holstein cattle. 相似文献
5.
Yayoi OTSUKA-YAMASAKI Osamu INANAMI Haruka SHINO Reeko SATO Masahiro YAMASAKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(2):315
Hereditary methemoglobinemia associated with nicotinamide adenine dinucleotide-cytochrome b5 reductase (b5R) deficiency is a rare autosomal recessive disorder in animals. Recently, nonsynonymous b5R gene (CYB5R3) variants have been reported to be associated with canine and feline hereditary methemoglobinemia. However, the underlying molecular mechanisms of canine and feline methemoglobinemia caused by these nonsynonymous variants have not yet been reported. Previously, we reported a Pomeranian dog family with hereditary methemoglobinemia, carrying CYB5R3 mutation of an A>C transition at codon 194 in exon 7, replacing an isoleucine residue with leucine (p.Ile194Leu). In this study, we investigated the enzymatic and structural properties of the soluble form of wild-type and Ile194Leu canine b5Rs to characterize the effects of this missense mutation. Our results showed that the kinetic properties of the mutant enzyme were not affected by this amino acid substitution. The secondary structure of the wild-type and Ile194Leu b5Rs detected by circular dichroism showed a similar pattern. However, the mutant enzyme exhibited decreased heat stability and increased susceptibility to trypsin hydrolysis. Moreover, the thermostability and unfolding measurements indicated that the mutant enzyme was more sensitive to temperature-dependent denaturation than the wild-type b5R. We concluded from these results that unstable mutant enzyme properties with normal enzymatic activity would be associated with hereditary methemoglobinemia in the Pomeranian dog family. 相似文献
6.
Masahito SUGIMOTO Waka SAITO Motoki OOI Yukinobu SATO Toshiro SAITO Kiyokazu MORI 《Animal Science Journal》2006,77(6):587-594
Six ruminally cannulated Wagyu (Japanese Black) steers (average initial bodyweight (BW) 387 ± 29 kg) were used in a split‐plot design experiment, comprising a 3 × 3 Latin square design (whole plot) and a randomized block design (subplot). The whole plot treatments were three different feeding levels of supplemental diet, fed at 0.2, 0.4 and 0.6% of BW, on a dry matter (DM) basis. Subplot treatments were two different supplemental diets: a potato pulp silage‐based diet (PPS) and a grain‐based diet (GRAIN). Chopped, medium‐quality cool‐season grass hay (predominately Timothy, Phleum pratense L) was fed daily at 0.7% BW (on a DM basis) as the basal diet. Each period consisted of 21 d, which included 11 d of adaptation to the diets and 10 d of the collection period. Chromium oxide was used as an indigestible marker. In situ forage degradation was measured using the nylon bag technique. The dry matter intake increased (linear; P < 0.01) as the feeding level increased and was not affected by the diet. Digestibility was not affected by any treatments. The GRAIN diet tended to decrease the rate of in situ forage degradation as the feeding level increased, but this trend was not found in the steers fed the PPS diet. Steers fed the GRAIN diet had a lower (P < 0.1) ruminal pH compared with steers fed the PPS diet. Ruminal pH was not significantly affected by feeding level; however, it was numerically higher for steers supplemented at 0.2% per BW than that for the steers supplemented above 0.4% per BW due probably to the higher starch intake. The total volatile fatty acids concentration numerically increased as the feeding level increased and was not affected by the diet. Increasing the feeding level decreased (linear, P < 0.01) the proportion of acetate. Neither diet nor the feeding level had any effects on the proportion of ruminal propionate. The results suggested that, for steers fed the PPS diet, there are not adverse effects on forage digestion in the rumen that occur as the feeding level is increased. 相似文献
7.
Kikumi OGIHARA Ken ONDA Reiichiro SATO Yuko NAYA Hideharu OCHIAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):85-88
L-type amino acid transporter
1 (LAT1), the first isotype of amino acid transport system L, transports aromatic and
branched amino acids pivotal for fundamental cellular activities such cellular growth and
proliferation. LAT1 expression was high only in the brain in contrast to its limited
distribution and low level of expression in normal tissues. We found potent LAT1
expression in canine caput epididymis by quantitative RT-PCR and Western blotting
analysis. Immnuno-histochemical examination revealed observable LAT1 in microvillous
epithelial cells. 相似文献
8.
Kazuyuki SUZUKI Toshio SHIMAMORI Ayano SATO Kenji TSUKANO Masakazu TSUCHIYA Jeffrey LAKRITZ 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(8):977-979
The aim of the present study was to compare the ability of the commercially available portable test system (PTSTM) to detect endotoxin activity in bovine serum, with that of the traditional LAL-kinetic turbidimetric (KT) and chromogenic (KC) assays. Prior to testing, serum samples, which were obtained from endotoxin-challenged cattle, were diluted 1:20 in endotoxin-free water and heated to 80°C for 10 min. The performance of the PTSTM was not significantly different from that of the traditional LAL-based assays. The results using PTSTM correlated with those using KT (r2=0.963, P<0.001) or KC assays (r2=0.982, P<0.001). Based on these findings, the PTSTM could be applied as a simplified system to assess endotoxin activity in bovine serum. 相似文献
9.
Bhuminad DEVKOTA Masahiro TAKAHASHI Saori SATO Kouya SASAKI Atsushi UEKI Takeshi OSAWA Masahiro TAKAHASHI Norio YAMAGISHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):875-878
Blood samples were obtained sequentially from 10 dairy cows around the time of
parturition to assess plasma fluctuations in estradiol-17β (E2) levels in
association with those of several bone resorption markers. Plasma E2
concentration increased sharply a few days prepartum and decreased quickly after
parturition. In terms of bone resorption markers, the plasma level of tartrate-resistant
acid phosphatase isoform 5b (TRAP5b) rose significantly, commencing 1 week prepartum, and
was maintained at this level to a few days postpartum. The plasma concentration of
carboxyterminal collagen cross-links of type-I collagen (CTx) increased significantly
after parturition. These observations suggest that osteoclast-mediated bone resorption was
activated after parturition when plasma E2 concentrations decreased. 相似文献