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排序方式: 共有57条查询结果,搜索用时 31 毫秒
1.
Haruyuki HIRATA Temidayo OMOBOWALE Olufunke ADEBAYO Nodoka ASANUMA Asako HARAGUCHI Yoshiki MURAKAMI Kodai KUSAKISAKO Keiko IKEDA Mitsuhiko ASAKAWA Kazuyuki SUZUKI Chiaki ISHIHARA Hiromi IKADAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(3):338
The present study examined the presence of Babesia parasites in 104 domestic dogs in Nigeria. Sequentially, Babesia parasites infecting domestic dogs underwent genetic and phylogenetic analyses. The results of nested PCR based on the Piroplasmida 18S rRNA gene illustrated that 13.5% (14/104) of the samples were positive. The obtained positive samples determined the nucleotide sequences of the 18S rRNA genes. In the genetic and phylogenetic analyses, four of five nucleotide sequences were similar to Babesia canis rossi, and one sample exhibited a close similarity to a Babesia sp. isolated from a raccoon in Hokkaido, Japan. The present study revealed the widespread presence of B. canis rossi among domestic dogs in Nigeria. 相似文献
2.
Shintaro TOMURA Atsushi TOSHIMA Akira NOMURA Masahiko HIRATA Tetsushi YAMAGAMI Yumiko KAGAWA Tsuyoshi KADOSAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(3):439
An 11-year-old neutered male Domestic Shorthair cat presented with a 3-month history of hypoglycemia, two episodes of seizure, and intermittent tick-like signs. Serum biochemistry revealed severe hypoglycemia associated with high insulin concentrations. Dynamic abdominal computed tomography (CT) indicated two pancreatic masses, which were enhanced most during the late arterial phase but had different degrees and variations of attenuation. Partial pancreatectomy was performed. Histopathology and immunohistochemistry confirmed that one mass was an insulinoma and the other was an ectopic splenic tissue, consistent with the differences in imaging findings. When an intrapancreatic lesion with hyper-attenuation on dynamic abdominal CT is detected, not only insulinoma or metastasis of malignancies but also intrapancreatic accessory spleen (IPAS) should be considered as differential diagnoses. 相似文献
3.
新入侵害虫螺旋粉虱的田间药效试验及防效 总被引:8,自引:4,他引:8
螺旋粉虱(Aleurodicus disperses Russell)是新入侵我国海南省的l种外来有害生物,对农业生产和生态环境具有较大危害性.田间防治研究结果表明:10%(质量分数,以下各农药浓度均为质量分数)联苯菊酯乳油、10%高效灭百可乳油、4.5%高效氯氰菊酯乳油等3种菊酯类杀虫剂对螺旋粉虱均表现出良好的速效性和持效性,其中又以10%高效灭百可乳油防效最高,为98.32%~100%;40%乐果乳油、30%敌百虫乳油和40%乙酰甲胺磷乳油等3种有机磷类农药的防效以40%乙酰甲胺磷乳油较好,但都低于上述3种菊酯类农药的防效;98%巴丹粉剂速效性较好,持效性较低;18%杀虫双水剂的速效性和持效性均较差. 相似文献
4.
5.
To clarify the relationship between the phylogeny and infectivity of isolates of Podosphaera fuliginea s. lat. (= Sphaerotheca fuliginea s. lat.) from cosmos and cucumber, more than 50 powdery mildew isolates from these two plants were subjected to nucleotide sequencing
or PCR-RFLP analysis of the rDNA internal transcribed spacer (ITS) region and cross-inoculation tests. The isolates from both
cosmos and cucumber are genetically monotypic, and there are six nucleotide substitutions in the rDNA ITS region between isolates
from cosmos and cucumber. Cross-inoculation tests of these isolates revealed that isolates from cosmos are not pathogenic
on cucumber. Although isolates from cucumber produced conidia on leaves of cosmos in the laboratory, the conidial density
was much lower than that from isolates from cosmos. This result, as well as the fact that the cucumber strain was not isolated
from cosmos in fields, suggests that isolates from cucumber do not infect cosmos in the field. Therefore, powdery mildews
on cosmos and cucumber can be regarded to have become specialized for their hosts both genetically and pathogenically. The
present study reconfirms the close relationship between phylogeny and infectivity of powdery mildew fungi. Host specialization
may be a trigger that causes genetic divergence of powdery mildew fungi.
Received 28 June 2000/ Accepted in revised form 4 September 2000 相似文献
6.
Kenji UCHIDA Masahiro HIRATA Hidemasa MOTOSHIMA Tadasu URASHIMA Ikichi ARAI 《Animal Science Journal》2007,78(6):650-658
The traditional fermented dairy products were collected from three nomadic families in Donto‐Govi prefecture in Mongolia (central Mongolia), and those microbiota were analyzed. These samples consist three of ‘airag’, two of ‘tarag’, two of ‘isgelen tarag’ and ‘qoormog’, and some cheeses. In airag, Lactobacillus (L.) helveticus, L. kefiri, and Saccharomyces (S.) dairensis were common, and L. paracasei, L. plantarum, L. farciminis, S. cerevisiae, Issachenkia (I.) orientalis, Kluyveromyces (K.) wickerhamii were also found. In tarag, isgelen tarag and qoormog, L. helveticus, L. kefiri, L. fermentum, L. paracasei and L. acetotolerance were found. L. delbrueckii subsp. bulgaricus was also found in one tarag and one qoormog samples. Randomly amplified polymorphic DNA (RAPD) analysis showed that there were diversity in each L. helveticus family and products, and there were common strains found in airag and tarag in the same family. 相似文献
7.
Rei NAKANO Kazuya EDAMURA Tomohiro NAKAYAMA Kenji TESHIMA Kazushi ASANO Takanori NARITA Ken OKABAYASHI Hiroshi SUGIYA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(1):27-35
We investigated the in
vitro differentiation of canine bone marrow stromal cells (BMSCs) into voltage-
and glutamate-responsive neuron-like cells. BMSCs were obtained from the bone marrow of
healthy beagle dogs. Canine BMSCs were incubated with the basal medium for neurons
containing recombinant human basic fibroblast growth factor (bFGF; 100
ng/ml). The viability of the bFGF-treated cells was
assessed by a trypan blue exclusion assay, and the morphology was monitored. Real-time
RT-PCR was performed to evaluate mRNA expression of neuronal, neural stem cell and glial
markers. Western blotting and immunocytochemical analysis for the neuronal markers were
performed to evaluate the protein expression and localization. The Ca2+
mobilization of the cells was evaluated using the Ca2+ indicator Fluo3 to
monitor Ca2+ influx. To investigate the mechanism of bFGF-induced neuronal
differentiation, the fibroblast growth factor receptor inhibitor, the phosphoinositide
3-kinase inhibitor or the Akt inhibitor was tested. The bFGF treatment resulted in the
maintenance of the viability of canine BMSCs for 10 days, in the expression of neuronal
marker mRNAs and proteins and in the manifestation of neuron-like morphology. Furthermore,
in the bFGF-treated BMSCs, a high concentration of KCl and L-glutamate induced an increase
in intracellular Ca2+ levels. Each inhibitor significantly attenuated the
bFGF-induced increase in neuronal marker mRNA expression. These results suggest that bFGF
contributes to the differentiation of canine BMSCs into voltage- and glutamate-responsive
neuron-like cells and may lead to the development of new cell-based treatments for
neuronal diseases. 相似文献
8.
9.
Takeshi OHTA Shuichi KIMURA Masaya HIRATA Takahisa YAMADA Toshie SUGIYAMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1327-1330
The Spontaneously Diabetic Torii (SDT) fatty rat, a model for obese type 2 diabetes,
shows bone quantitative abnormalities, namely low bone mineral density (BMD). The
objective of this study was to evaluate bone morphological changes, in particular
identifying the bone qualitative abnormalities, in the SDT fatty rat. Male SDT fatty rats
showed increases in total trabecular area and trabecular number and decreases in
trabecular thickness in cancellous bones of the proximal tibia, indicating trabecular
miniaturization. The SDT fatty rat is useful for investigation of pathophysiological
changes in bone quality in diabetic osteoporosis. 相似文献
10.
教学活动的设计和使用成为英语课堂一个十分重要的环节,对最终的教学效果起不可或缺的作用.本文试从英语课堂教学活动的设计和活动方法两个方面,从教师的角度对该问题进行了初步探讨,对英语教师的课堂活动设计有一定参考作用. 相似文献