Effect of Bone Marrow Mesenchymal Stem Cells Transplantation on the Mammary Tissue Development and Serum Hormone Level of Saanen Dairy Goat

The study was aimed to analyze the effect of transplanted bone marrow mesenchymal stem cells (BMSCs) on the mammary gland development and serum hormone level of Saanen dairy goat.16 healthy goats with 2 months old and similar body weight were randomly divided into control group and experimental group. The BMSCs which was amplified to the P6 generation was injected to the goat via jugular vein,and the control group was injected with the same amount of normal saline. Feeding and management of the two groups were the same. Blood samples were collected monthly,and mammary gland tissue samples were collected at 3,5 and 7 months old. Frozen sections of mammary gland tissue was prepared, the ducts and acinus numbers were observed and recorded. The results showed that after transplantation of BMSCs,at 5 and 7 months old,the ducts and acinus number of goat in experimental group were significantly higher than that of control group (P<0.05).At 5,7,8 and 9 months old,the serum E₂ level of goat in experimental group was significantly higher than that of control group (P<0.05);At 5 to 9 months old,the serum P level in experimental group was significantly higher than that of control group (P<0.05).At 7 to 9 months old,the serum PRL and INS levels in experimental group were significantly higher than that of the control group (P<0.05);At 8 and 9 months old,the serum levels of GnRH level in experimental group was significantly higher than that of the control group (P<0.05).The serum LEP,CRH and ACTH levels were higher than that of the control group at the whole test process,but the difference was not significant (P>0.05).In conclusion, injection of BMSCs could increase the duct and acinus number of dairy goat mammary,improve the hormone level in serum,and promote mammary gland development.     

奶山羊乳腺上皮细胞与骨髓间充质干细胞混合培养下最适共培养体系的建立

[目的]研究乳腺上皮细胞与骨髓间充质干细胞混合培养促进细胞增殖作用的机制,提高奶山羊乳腺上皮增殖水平.[方法]采用分离培养的1月龄健康萨能奶山羊乳腺上皮细胞和骨髓间充质干细胞,分别设乳腺上皮细胞单纯培养组和不同比例的乳腺上皮细胞与骨髓间充质于细胞混合培养组,培养24、48、72、96、120和168 h时观察各组细胞的生长状态并检测细胞增殖率和贴壁率.[结果]混合培养组增值率和贴壁率比单纯培养组高,且细胞扁平时间晚于单纯培养组,混合培养体系可促进BMEC的增殖,减缓细胞凋亡.当BMEC与BMSCs以2∶1的比例混合培养到96 h时细胞的增值率和贴壁率最高.[结论]奶山羊乳腺上皮细胞和骨髓间充质干细胞共培养体系能够减缓乳腺上皮细胞的凋亡,促进乳腺上皮细胞增殖,提高细胞贴壁率.最佳共培养浓度为2:1,最佳作用时间为96 h.     

移植骨髓间充质干细胞对萨能奶山羊乳腺组织发育及血清激素水平的影响

试验旨在研究移植骨髓间充质干细胞（bone marrow mesenchymal stem cells,BMSCs）对萨能奶山羊乳腺发育的影响。选取体重接近的2月龄健康萨能奶山羊16只,随机分为对照组和试验组。将扩增至P6代的BMSCs经颈静脉注射至奶山羊体内,对照组注射等量生理盐水,两组间饲养管理均相同。每月进行空腹采血,并分别在3、5和7月龄采集乳腺组织样品,制备乳腺组织冰冻切片,观察记录各组乳腺组织导管数和腺泡数。结果显示,移植BMSCs后,5和7月龄时,试验组奶山羊的导管数、腺泡数均显著高于对照组（P<0.05）。在5、7、8、9月龄时,试验组奶山羊血清中雌激素（E₂）水平显著高于对照组（P<0.05）;在5~9月龄时,试验组血清中孕酮（P）水平显著高于对照组（P<0.05）;在7~9月龄时,试验组血清中催乳素（PRL）和胰岛素（INS）水平均显著高于对照组（P<0.05）;8、9月龄时,试验组血清中促性腺激素释放激素（GnRH）水平显著高于对照组（P<0.05）;整个试验过程中试验组血清中瘦素（LEP）、促肾上腺皮质激素释放激素（CRH）和促肾上腺皮质激素（ACTH）水平均高于对照组,但差异不显著（P>0.05）。综上所述,注射BMSCs能增加奶山羊乳腺导管数和腺泡数,提高血清中激素水平,从而促进乳房发育。