First Established Pregnancies in Mediterranean Italian Buffaloes (Bubalus bubalis) Following Deposition of Sexed Spermatozoa near the Utero Tubal Junction

At the time of AI following Ovsynch protocol, a total of 51 buffaloes were randomly divided in a first group (n = 30) subjected to conventional AI into the uterine body with 20 million non-sex sorted frozen-thawed spermatozoa, while a second group (n = 21) was inseminated near the utero-tubal junction (UTJ) ipsilateral to the ovary carrying the preovulatory follicle with 2.5 million live (4 million total) sex-sorted frozen-thawed spermatozoa. The semen used for flowcytometric sorting was collected and processed on a farm in Italy, and then shipped to a laboratory in Germany. Eleven buffaloes were inseminated with X-chromosome bearing spermatozoa and 10 with Y-chromosome bearing spermatozoa. Conception rates after conventional and UTJ inseminations were 43.3% (n = 13) and 42.8% (n = 9) respectively (p = 0.97). Eight of the nine foetuses obtained after insemination with sexed spermatozoa corresponded to the sex as predicted by the cell sorting procedure (five male and four female foetuses by ultrasound vs six male and three female foetuses by cell sorting). In conclusion, for the first time buffalo semen has been successfully subjected to procedures for flowcytometric sperm sorting and freezing. Low doses of sexed spermatozoa have been deposited near the UTJ giving conception rates similar to those of conventional AI with full dose.     

Isolating the effect of soil properties on agricultural soil greenhouse gas emissions under controlled conditions

Agricultural soils are important sources of greenhouse gases (GHGs). Soil properties and environmental factors have complex interactions which influence the dynamics of these GHG fluxes. Four arable and five grassland soils which represent the range of soil textures and climatic conditions of the main agricultural areas in the UK were incubated at two different moisture contents (50 or 80% water holding capacity) and with or without inorganic fertiliser application (70 kg N ha⁻¹ ammonium nitrate) over 22 days. Emissions of N₂O, CO₂ and CH₄ were measured twice per week by headspace gas sampling, and cumulative fluxes were calculated. Multiple regression modelling was carried out to determine which factors (soil mineral N, organic carbon and total nitrogen contents, C:N ratios, clay contents and pH) that best explained the variation in GHG fluxes. Clay, mineral N and soil C contents were found to be the most important explanatory variables controlling GHG fluxes in this study. However, none of the measured variables explained a significant amount of variation in CO₂ fluxes from the arable soils. The results were generally consistent with previously published work. However, N₂O emissions from the two Scottish soils were substantially more sensitive to inorganic N fertiliser application at 80% water holding capacity than the other soils, with the N₂O emissions being up to 107 times higher than the other studied soils.     

DRYING AND CONDITIONING OF DIRECT–HARVESTED TIMOTHY SEED

An experiment is described in which S48 timothy seed, direct harvested at 3 stages of ripeness, was dried in layers 6 in. deep, at air temperatures from 75–135° F, immediately after harvest and after periods of from 1 to 3 days conditioning with cold air. Germination results for non-conditioned seed showed good agreement with those obtained in the previous year. The quality of seed hulled during threshing was inferior to that remaining unhulled after threshing, but the latter was more susceptible to high air temperature during drying. The relationship of the seed obtained in this experiment to commercial seed is discussed and a scheme for the practical application of the results will be presented in a subsequent paper.     

INVESTIGATIONS ON THE DRYING OF HERBAGE AT TEMPERATURES UP TO 50°C*

Experiments to determine the exposed layer drying rate of cut leaves and stems of a tetraploid (Sabel) and diploid (S23) ryegrass are described. Drying was achieved by passing air through the crop; the changes in weight were monitored continuously. Airflow was fixed throughout the experiments at 37·5 ft/min (0·190 m/s) and the temperatures were controlled in the range 25°C to 50°C. Comparisons were also made of the drying rates at different stages of maturity. Drying curves were determined and moisture content was shown to decay exponentially with time, the relationship approximating closely to a two term exponential.     

Response to immunotherapy in six related horses with urticaria secondary to atopy

Urticaria secondary to atopy may be a familial problem in some horses. Immunotherapy using a vaccine containing antigens selected on the basis of history and results of intradermal testing can be an effective method of managing atopy in horses; a response to therapy may be seen within 2 months.     

Effects of growth-regulating chemicals on two cultivars of Mid-Century Hybrid lily

The effects on growth and flowering of Mid-Century Hybrid lilies ‘Enchantment’ and ‘Joan Evans’ of soil drench applications of ancymidol at 0, 5, 10 and 15 ppm have been compared with those produced by drenches of chlormequat chloride, chlorphonium chloride and ethephon at 25 000, 1000 and 1000 ppm, respectively.Ancymidol inhibited stem growth more effectively than a 5000-fold greater quantity of chlormequat chloride. Chlorphonium chloride had little dwarfing effect, and ethephon, although producing height reductions similar to those obtained with 5 ppm ancymidol, delayed flowering slightly in both cultivars (significantly in ‘Enchantment’), and adversely affected overall plant appearance.Ancymidol reduced the lateral spread of foliage, increased the proportion of the total stem length bearing dead leaves, did not significantly delay flowering in either cultivar and appeared to favour the initiation and survival of secondary flowers in ‘Enchantment’.