Root growth, water potential, and yield of irrigated rice

Root length density (Lv), leaf water potential (Ψ leaf) and yield of rice were studied in 1983 and 1984 on a Phool bagh clay loam (Typic Haplaquoll) and on a Beni silty clay loam (Aquic Hapludoll) in the Tarai region of Uttar Pradesh under naturally fluctuating shallow (0.07–0.92 m) and medium-depth (0.13–1.26 m) water table conditions with six water regimes ranging from continuous submergence under 0.05 m ± 0.02 m (Ic) to completely rainfed (Io). In irrigation treatments, Ic1, Ic3, Ic5, and Ic7, 0.07 m irrigation was applied on days 1, 3, 5, and 7 respectively, after the disappearance of ponded water. Maximum rooting depth (0.55 m in the shallow and 0.65 m in the medium-depth water table) was attained at the dough stage (125 days after transplanting) and was more strongly influenced by fluctuations in water table depth than by the water regime. For wet regimes (Ic1–Ic5), roots were concentrated at and above the water table interface and had greater horizontal development, whereas in dry regimes, (Ic7 and Io) they were concentrated in lower horizons and had a more vertical distribution. Like Lv, Ψ leaf was not significantly affected by water regime up to 90–95 days after rice transplanting but was significantly affected thereafter, except for Lv beneath 0.2 m–0.25 m. Grain yields with irrigation treatments Ic1 and Ic3 under shallow and Ic1 under medium-depth water table conditions were not significantly different from those under continuous submergence, but there was a (nonsignificant) trend to lower yield with less water. However, differences among the wet regimes (Ic, Ic1, and Ic3) were small (141–490 kg ha^–1) under shallow and 413–727 kg ha^–1 under medium-depth water table conditions. The results demonstrate that optimum yield (5500–6000 kg ha^–1) could be obtained under Tarai conditions by adopting an intermittent irrigation schedule of 3–5 days after the disappearance of ponded water under shallow, and of 1–3 days under medium-depth water table conditions, in place of continuous submergence. Received: 26 February 1996     

Potential,constraints and applications of in vitro methods in improving grain legumes

Grain legumes, the important constituents of sustainability‐based cropping systems and energy‐limited vegetarian diets have long been the subject of scientific research. Tremendous technological strides were made in the so‐called orphan crops, in terms of both varietal improvement and generation of basic information. Despite recalcitrancy and high genotype dependency, in vitro culture techniques such as organogenesis, in vitro mutagenesis, embryo rescue and in vitro gene transfer have been deployed for improvement of several grain legumes and these played an important role in introgression of desirable genes from related and distant species and creation of additional genetic variability. Stable and reproducible regeneration protocols resulted in the development of genetically modified chickpea, pigeon pea, cowpea, mungbean, etc., while embryo rescue was deployed successfully for recovery of interspecific recombinants, a few of them exploited for the development of commercial cultivars. Nevertheless, doubled haploidy witnessed limited success and protoplast regeneration and in vitro mutagenesis remained of academic interest. The present review focuses on the progress, achievements, constraints and perspectives of using in vitro technology in grain legume improvement.     

Capturing genetic variability and selection of traits for heat tolerance in a chickpea recombinant inbred line (RIL) population under field conditions

Chickpea is the most important pulse crop globally after dry beans. Climate change and increased cropping intensity are forcing chickpea cultivation to relatively higher temperature environments. To assess the genetic variability and identify heat responsive traits, a set of 296 F_8–9 recombinant inbred lines (RILs) of the cross ICC 4567 (heat sensitive) × ICC 15614 (heat tolerant) was evaluated under field conditions at ICRISAT, Patancheru, India. The experiment was conducted in an alpha lattice design with three replications during the summer seasons of 2013 and 2014 (heat stress environments, average temperature 35 °C and above), and post-rainy season of 2013 (non-stress environment, max. temperature below 30 °C). A two-fold variation for number of filled pods (FPod), total number of seeds (TS), harvest index (HI), percent pod setting (%PodSet) and grain yield (GY) was observed in the RILs under stress environments compared to non-stress environment. A yield penalty ranging from 22.26% (summer 2013) to 33.30% (summer 2014) was recorded in stress environments. Seed mass measured as 100-seed weight (HSW) was the least affected (6 and 7% reduction) trait, while %PodSet was the most affected (45.86 and 44.31% reduction) trait by high temperatures. Mixed model analysis of variance revealed a high genotypic coefficient of variation (GCV) (23.29–30.22%), phenotypic coefficient of variation (PCV) (25.69–32.44%) along with high heritability (80.89–86.89%) for FPod, TS, %PodSet and GY across the heat stress environments. Correlation studies (r = 0.61–0.97) and principal component analysis (PCA) revealed a strong positive association among the traits GY, FPod, VS and %PodSet under stress environments. Path analysis results showed that TS was the major direct and FPod was the major indirect contributors to GY under heat stress environments. Therefore, the traits that are good indicators of high grain yield under heat stress can be used in indirect selection for developing heat tolerant chickpea cultivars. Moreover, the presence of large genetic variation for heat tolerance in the population may provide an opportunity to use the RILs in future-heat tolerance breeding programme in chickpea.     

Heavy infection of Ichthyophthirius multifiliis in striped catfish (Pangasianodon hypophthalmus,Sauvage 1878) and its treatment trial by different therapeutic agents in a control environment

ABSTRACT

Striped catfish (Pangasianodon hypophthalmus) fingerlings were stocked (60 fishes/m³) in cemented square tanks for ornamental fishery purposes at the College Fish Farm in Mangalore. A total of 400 fishes with a mean weight (g) ranging from 3.24 ± 1.21 to 6.70 ± 1.13 and a mean length (cm) of 7.50 ± 0.94 to 9.50 ± 1.10 were examined. A severe outbreak of salt-like granule white spots was found on the body surface. Ichthyophthirius multifiliis, a ciliate pathogen, was identified as the causative agent by clinical signs, wet mount, and histopathological observations. Infected fishes were transferred and equally distributed to the 0.45 m³ glass aquaria and treated with three treatments: (T1) methylene blue + salt; (T2) raising temperature with salt; and (T3) formalin + malachite green. The best fingerling survival (55 ± 9.36%) was obtained by elevated water temperature with salt in T2.     

Clinical exercise testing in the normal Thoroughbred racehorse

To evaluate normal cardiorespiratory and metabolic responses of Thoroughbred horses to a standardised treadmill exercise test, we examined 28 horses ranging in age from 1 to 4 years. The group consisted of eight yearlings, eight 2-year-olds and twelve 3 and 4-year-olds. All horses except the yearlings were in training, and either racing or ready to race, at the time of examination. None of the horses had histories of performance problems. On the first day the horses received a full physical examination, resting electrocardiogram, upper respiratory tract endoscopy and either one or two acclimatisation runs on the treadmill. The following day they were given an exercise test on a treadmill inclined at 6 degrees (+10% slope). The test consisted of 3 min at 4 m/sec, 90 sec at 6 m/sec and 60 sec intervals at 8, 10, 11, 12 and 13 m/sec. During the last 15 sec of each step, blood samples were collected for plasma lactate determination, expired respiratory gases were obtained using an open flow mask system for measurement of oxygen uptake, and heart rate was measured using telemetry electrocardiogram. From these measurements, various derived values were calculated, which have been used by others as indices of exercise capacity. These values included: V200 (speed at HR of 200 bpm), VHRmax (speed at which horses reached maximum HR), VO2-200 (oxygen uptake at a HR of 200 bpm), VO2max (maximum oxygen uptake), VLA4 (speed at which horses reached a plasma lactate of 4 mmol/l) and HRLA4 (HR at which horses reached a plasma lactate of 4 mmol/l). The yearlings had significantly lower values than the older age groups for most of the derived values.(ABSTRACT TRUNCATED AT 250 WORDS)     

Genetic variability for resistance to sorghum downy mildew (Peronosclerospora sorghi) and Rajasthan downy mildew (P. heteropogoni) in the tropical/sub-tropical Asian maize germplasm

Downy mildews cause considerable damage to maize (Zea mays L.) worldwide, particularly in the tropical Asia. We have evaluated a set of 42 tropical/sub-tropical maize inbred lines developed in different countries in Asia (India, Thailand and Philippines), and Mexico, for analysing the genetic variability for resistance to sorghum downy mildew [Peronosclerospora sorghi; SDM] and Rajasthan downy mildew [P. heteropogoni; RDM]. Experiments were carried out in replicated trials under artificial infection in field conditions against SDM and RDM at Mandya in Karnataka, India, and Udaipur in Rajasthan, India, respectively, during 1999 and 2000. The study resulted in identification of five inbred lines offering consistent and strong resistance to both SDM and RDM, while several inbred lines revealed resistance only to RDM. It was also revealed that the SDM-resistant inbreds are invariably resistant to RDM, while the RDM-resistant inbreds might show differential responses to the SDM. The maize inbred lines identified in this study with broad-spectrum resistance to downy mildews could be potentially useful for basic and applied research work on downy mildews in tropical Asia. This revised version was published online in August 2006 with corrections to the Cover Date.     

Effect of surface crusting on emergence of soybean (Glycine max L. Merr) seedlings II. Influence of tillage treatment,planting method and time of crust formation

The influence of tillage treatment and the time of crust formation after planting on the degree of surface crusting and seedling emergence of soybean (Glycine max L. Merr.), planted on the flat and on ridges, are described. Crusts were developed artificially at specific times after planting. Under non-crusted conditions (control), more rapid drying of the soil in the seed zone occurred in conventionally-tilled, ridge-planted (Tcr) and minimum-tilled, flat-planted (Tmf) seedbeds compared with excessively and conventionally-tilled, flat-planted (Tef and Tcf) seedbeds. The formation of surface crusts induced a reduction in moisture loss from the seed zone. On drying, large crust blocks with a smooth surface developed in Tef plots whereas, in Tmf plots, small crust blocks with a rough surface appeared. Both the rate of emergence and the ultimate emergence of soybeans were in the order Tef > Tcf > Tcr > Tmf under non-crusted conditions and, on average, in the order Tcr > Tcf > Tef > Tmf under crusted conditions. Crusts which formed within two days of sowing were more injurious because these were hard and impenetrable, and were present at the normal time of seedling emergence. Crusts induced 72 h after planting had less effect on seedling emergence because, by the time they could form, emergence was already almost completed.     

Response of Paddy (Oryza sativa) as Influenced by Zinc Application and Available Zinc Status of Soils

The influence of zinc application on yield, Zn concentration and its uptake by paddy crop (Oryza sativa) was studied in a pot experiment in alluvial soils from Gwalior district. All the soils having less than 3.0 ppm 0.1 N HCl-extractable soil Zn showed significant yield response to application of Zn. The latter also resulted in significant increase in Zn concentration and its uptake by the crop. The plant tissue Zn was found to be significantly correlated at 1 % level with the soil Zn (r=0.82) and Bray's per cent grain yield (r=0.82). Critical level Zn in rice plant taken from top 15 cm of the plant was worked out by statistical procedure of Cate and Nelson and found to be 13.5 ppm below which response to applied Zn is expected.