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排序方式: 共有81条查询结果,搜索用时 15 毫秒
1.
Aida Raio Raffaele Peluso Xavier Nesme Astolfo Zoina 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(2):163-174
Agrobacteria were previously isolated from tumors developing on branches and aerial and hypogeous roots of weeping fig plants in Italy and in The Netherlands. A representative group of 48 strains was analyzed by PCR–RFLP of 16S and 16S + IGS ribosomal regions, PCR–RFLP of six Ti plasmid (pTi) regions and characterized for plasmid content. Two groups of agrobacteria were separated by cluster analysis of PCR–RFLP profiles of rrs gene: seventeen strains were similar to the new species Agrobacterium larrymoorei, while the remaining strains were included within the agrobacterium biovar 1 group. Sixteen different plasmid profiles from one to five plasmids were observed. In addition, 21 ribotypes and 20 pTi structures were arranged in many different combinations, showing that fig agrobacteria were characterized by a wide heterogeneity. A general lack of correlation between strain ribotypes and plasmid content was observed. 相似文献
2.
Phytate degradation by lactic acid bacteria and yeasts during the wholemeal dough fermentation: a 31P NMR study 总被引:1,自引:0,他引:1
Reale A Mannina L Tremonte P Sobolev AP Succi M Sorrentino E Coppola R 《Journal of agricultural and food chemistry》2004,52(20):6300-6305
myo-Inositol hexaphosphate (IP6) is the main source of phosphorus in cereal grains, and therefore, in bakery products. Different microorganisms such as yeasts and lactic acid bacteria have phytase enzymes able to hydrolyze IP6 during the wholemeal breadmaking. In this paper, the phytase activity of Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus curvatus, and Saccharomyces cerevisiae strains, isolated from southern Italian sourdoughs, is assayed using the (31)P NMR technique. The sourdough technology based on the use of lactic acid bacteria in the breadmaking is finally suggested. 相似文献
3.
Napolitano A Morales F Sacchi R Fogliano V 《Journal of agricultural and food chemistry》2008,56(6):2034-2040
In this paper the relationship between virgin olive oil (VOO) phenol compounds and the formation of acrylamide in potato crisps was investigated. The phenol compositions of 20 VOO samples were screened by LC-MS, and 4 oils, characterized by different phenol compound patterns, were selected for frying experiments. Slices of potatoes were fried at 180 degrees C for 5, 10, and 15 min, and acrylamide content was determined by LC-MS. Results demonstrated that VOO phenolic compounds are not degraded during frying, and crisp color was not significantly different among the four VOOs. Acrylamide concentration in crisps increased during frying time, but the formation was faster in the oil having the lowest concentration of phenolic compounds. Moreover, the VOO having the highest concentration of ortho-diphenolic compounds is able to efficiently inhibit acrylamide formation in crisps from mild to moderate frying conditions. It was concluded that the use of ortho-diphenolic-rich VOOs can be proposed as a reliable mitigation strategy to reduce acrylamide formation in domestic deep-frying. 相似文献
4.
Pacemaker implantation in a ferret (Mustela putorius furo) with third-degree atrioventricular block.
David Sanchez-Migallon Guzman Joerg Mayer Raffaele Melidone Robert J McCarthy Emily McCobb Anoop Kavirayani John E Rush 《Veterinary Clinics of North America: Exotic Animal Practice》2006,9(3):677-687
A 7.5-year-old castrated male ferret (Mustela putorius furo) was diagnosed with third-degree atrioventricular (AV) block. A monopolar epicardial pacemaker system was implanted, resulting in a regular, paced cardiac rhythm with third-degree AV block at 140 beats per minute. Over the next 2 months, the ferret developed anorexia, interstitial pneumonia, intermittent diarrhea, and hind-limb weakness and had a slow and progressive recovery. The ferret developed clinical signs of congestive heart failure 4 months after the surgery, resulting in its death 3 weeks later. Necropsy results attributed the death to cardiac failure due to extensive myocardial mineralization. To the authors' knowledge this is the first published report of surgical report of surgical pacemaker implantation in a ferret. 相似文献
5.
Mologni Omar Lyons C. Kevin Marchi Luca Amishev Dzhamal Grigolato Stefano Cavalli Raffaele Röser Dominik 《European Journal of Forest Research》2021,140(4):745-759
European Journal of Forest Research - The growing interest in accessing steep terrain is pushing the expansion of the operating range of ground-based machines through winch-assist technology.... 相似文献
6.
Screening RAPD primers for molecular taxonomy and cultivar fingerprinting in the genus Actinidia 总被引:3,自引:0,他引:3
Summary Eighty ten-base long arbitrary primers were tested for PCR-based DNA amplification of three species of the genus Actinidia (A. deliciosa the kiwifruit, A. chinensis, and A. kolomikta), with the aim of screening species-specific and genotype-specific markers.Of the 80 primers tested, 30 gave an average of 3.5 bands which were monomorphic within one or two species and absent in the remaining one(s), thus resulting in useful markers for taxonomic and phylogenetic purposes. None of the primers tested produced bands linked to sex. Twenty primers out of the twenty-five selected from a preliminary screening showed high levels of polymorphism, producing two to eleven patterns each from the 13 kiwifruit cultivars examined.We found the Stoffel fragment and the Taq polymerase were both suitable for RAPD analysis, the most noticeable difference being the smaller size of fragments (0.4–1.2 kb) produced by the former in comparison to the latter (1.0–3.4 kb). We tested also three different annealing temperatures (35, 37, and 39° C) and found the intermediate one best for number of amplified bands and reproducibility of results.Abbreviations 2-BE
2-butoxyethanol
- CTAB
hexadecyltrimethylammonium bromide
- MAS
Marker-Assisted-Selection
- PCR
Polymerase Chain Reaction
- RAPD
Random Amplified Polymorphic DNA
- RFLPs
Restriction Fragment Length Polymorphisms 相似文献
7.
Rudolf Hofer Reinhard Lackner Joachim Kargl Bertha Thaler Danilo Tait Leopoldo Bonetti Raffaele Vistocco Giovanna Flaim 《Water, air, and soil pollution》2001,125(1):189-200
Southern populations of the European minnow fromremote oligotrophic mountain lakes along an Alpinenorth-south transect accumulated more p,p DDE andPCBs than northern populations. As these semi-volatileorganochlorines predominantly evaporate in warmcountries, higher rates of condensation (deposition)are assumed to occur in the southern slopes of theAlps. The higher accumulation of lead and cadmium insouthern population is rather attributed to geogenicand specific environmental impacts than to atmosphericdeposition. Increasing liver ratios of [glutathionedisulfide]/[glutathione], a potential indicator foroxidative stress, from north to south reflect thegenerally higher toxic load at remote sites at thesouthern edge of the Alps. However, histopathologicalchanges in the liver did not correlate withaccumulated toxicants indicating that deposition doesnot lead to severe lesions but induces specificmechanisms for detoxification. 相似文献
8.
Raffaele Spinelli Natascia Magagnotti Carla Nati 《European Journal of Forest Research》2011,130(5):737-744
In Italy, poplar plantations represent an important source of wood products, and especially of high-quality veneer logs. These
plantations offer favourable conditions to the introduction of forest mechanisation, and especially easy access and industrial
management. However, owners fear that mechanised log-making may cause value losses, due to poor length measurement, log surface
damage and improper grading. This study compared the performance of manual and mechanised log-making on ten commercial operations,
in order to determine the occurrence and the severity of possible value losses due to product degrade. The study found that
length measurement errors are smaller for mechanised processing, while the frequency and severity of log surface damage are
the same for both treatments. In three cases out of five, mechanised log-making extracted the same value from the stems as
manual log-making: in the remaining two, differences were very small and went opposite directions. Significant differences
were found between operators, stressing the importance of operator skills and motivation. In fact, the log-making specifications
and the pricing structure applied to poplar processing are relatively simple and are unlikely to challenge the ability of
personnel and equipment. At present, most Italian poplar growers are unaware of the potential offered by modern technology,
whose intelligent use would boost value recovery and decrease harvesting cost. Significant benefits may derive from the introduction
of optimised bucking, which may help shifting to a more articulated and rewarding product strategy. 相似文献
9.
Carmela Gallo Giusi Barra Marisa Saponaro Emiliano Manzo Laura Fioretto Marcello Ziaco Genoveffa Nuzzo Giuliana dIppolito Raffaele De Palma Angelo Fontana 《Marine drugs》2020,18(12)
Immunotherapy takes advantage of the immune system to prevent, control, and eliminate neoplastic cells. The research in the field has already led to major breakthroughs to treat cancer. In this work, we describe a platform that integrates in vitro bioassays to test the immune response and direct antitumor effects for the preclinical discovery of anticancer candidates. The platform relies on the use of dendritic cells that are professional antigen-presenting cells (APC) able to activate T cells and trigger a primary adaptive immune response. The experimental procedure is based on two phenotypic assays for the selection of chemical leads by both a panel of nine tumor cell lines and growth factor-dependent immature mouse dendritic cells (D1). The positive hits are then validated by a secondary test on human monocyte-derived dendritic cells (MoDCs). The aim of this approach is the selection of potential immunotherapeutic small molecules from natural extracts or chemical libraries. 相似文献
10.
Raffaele S Farrer RA Cano LM Studholme DJ MacLean D Thines M Jiang RH Zody MC Kunjeti SG Donofrio NM Meyers BC Nusbaum C Kamoun S 《Science (New York, N.Y.)》2010,330(6010):1540-1543
Many plant pathogens, including those in the lineage of the Irish potato famine organism Phytophthora infestans, evolve by host jumps followed by specialization. However, how host jumps affect genome evolution remains largely unknown. To determine the patterns of sequence variation in the P. infestans lineage, we resequenced six genomes of four sister species. This revealed uneven evolutionary rates across genomes with genes in repeat-rich regions showing higher rates of structural polymorphisms and positive selection. These loci are enriched in genes induced in planta, implicating host adaptation in genome evolution. Unexpectedly, genes involved in epigenetic processes formed another class of rapidly evolving residents of the gene-sparse regions. These results demonstrate that dynamic repeat-rich genome compartments underpin accelerated gene evolution following host jumps in this pathogen lineage. 相似文献