Genetic relationship, inbreeding and generation interval in registered Gir cattle in Brazil

SUMMARY: Two-hundred random pedigrees of cows and fifty of sires born in 1987 from the Gir herdbook of the Brazilian Association of Zebu Breeders were analized. The bull Chave de Ouro DGR no. 2851 was the most influential animal with a 7% direct relationship to the breed. Total average inbreeding was 3.62 and 3.25 for the female and male sample, respectively. The subdivision of total inbreeding into current and non-current components resulted in values of 1.62, 1.25, 2.00 and 2.00 for the female and male sample, respectively. Long-term inbreeding was the principal component of non-current inbreeding, with values of 1.52% and 1.26%, respectively. Inbreeding due to strain formation strain due formation was less important (0.48% and 0.74%, respectively). The average generation interval was 8.02 years. The breed appears not yet to be subdivided into strains but this process may be starting. The average generation interval for the pedigree population was very high. Culling of old bulls could decrease this interval. ZUSAMMENFASSUNG: Verwandtschaft, Inzucht und Generationsintervall bei Gir Herdbuchrindern in Brasilien 200 zuf?llige Stammb?ume von Kühen und 50 von 1987 geborenen Stieren wurden vom Gir Herdbuch des brasilianischen Zebu Zuchtverbandes genommen. Der Stier Chave de Ouro DGR Nr. 2851 war das einflu?reichste Tier mit einer 7%igen direkten Verwandtschaft zur Rasse. Der gesamte Inzuchtkoeffizient war 3.6 und 3.25% für die weibliche und m?nnliche Stichprobe. Die Unterteilung der gesamten Inzucht in laufende und nicht laufende Komponenten resultierte in Werten von 1.62, 1.25, 2.0, 2.0 für die weibliche und die m?nnliche Stichprobe. Langfristige Inzucht war die Hauptursache für Nicht-Rezente Inzucht mit Werten von 1.5 und 1.26. Inzucht aufgrund von Linienbildung war weniger wichtig (0.48% und 0.74%). Das durchschnittliche Generationsintervall war 8.02 Jahre. Die Rasse scheint noch nicht in Linien unterteilt zu sein, aber dieser Proze? k?nnte begonnen haben. Das durchschnittliche Generationsintervall ist mit 8.02 Jahren sehr hoch. Nichtverwendung alter Stiere k?nnte dieses Intervall verkürzen.     

In situ provision of drinking water to grazing dairy cows improves milk production

AIMS: To determine the effect of providing water within the area grazed by dairy cows on milk yield and quality, compared to requiring cows to walk to a distant water trough, on a dairy farm in the Pampa region of Argentina during summer.

METHODS: Holstein dairy cows were allocated to two herds with similar parity, days in milk and milk production. They were grazed in one paddock that was divided in two, with a fixed water trough at one end. Cows were moved twice daily to grazing plots within the paddock. Control cows (n=66) could only access water from the fixed trough, whereas supplemented cows (n=67) also received water from a mobile trough within the grazing plot. Milk production of each cow, and water consumption of the two herds were measured daily over 62 days. Milk composition for each herd was determined weekly from Days 18 to 60 of the study, and grazing behaviour was observed between 08:00 and 16:00 hours on Days 11–15, 19–22 and 39–43.

RESULTS: Over the 62 days of the study, supplemented cows produced 1.39 (SE 0.11) L/cow/day more milk than Control cows (p=0.027). Estimated mean daily water intake was 50.4 (SE 2.1) L/cow/day for supplemented cows and 58.2 (SE 2.7) L/cow/day for Control cows (p=0.004). Percentage total solids in milk was higher for supplemented (12.5 (SE 0.06)%) than Control (12.4 (SE 0.04)%) cows (p=0.047). During the periods of behavioural observation, a higher percentage of cows in the water supplemented than the Control herd were observed in the grazing area (p=0.012).

CONCLUSIONS AND CLINICAL RELEVANCE: This preliminary study demonstrated that provision of water to dairy cows within the grazing plot was beneficial for milk production and composition, and may be associated with longer periods spent within the grazing area, during hot weather in the Pampa region of Argentina.     

Association between bovine digital dermatitis and annual periods of lower pluviometric precipitation in Central Brazil

Tropical Animal Health and Production - We aimed to follow the epidemiologic evolution of bovine digital dermatitis (BDD) and other podal diseases grouped in a dairy farm in Central Brazil between...     

Spermatic and oxidative profile of domestic cat (Felis catus) epididymal sperm subjected to different cooling times (24, 48 and 72 hours)

Cooling stored epididymal samples for several days allows facilities to transport and process genetic material post‐mortem. Improvements to this practice allow the preservation of sperm from domestic cats, which are the ideal study model for wild felids. However, the modifications in spermatic features and the oxidative profile are not fully understood in cats. This information is necessary for the development of biotechniques, such as new extenders for cryopreservation. Therefore, the purpose of this study was to evaluate the spermatic and oxidative profile in samples from the epididymal cauda of domestic cats cooled at 5°C for 24, 48 and 72 hr. Spermatozoa were collected from the epididymis cauda. Evaluations consisted of computer‐assisted sperm analysis (CASA), plasma membrane integrity (eosin/nigrosin), acrosome integrity (fast green/rose bengal), sperm morphology, sperm DNA integrity (toluidine blue), mitochondrial activity (3′3 diaminobenzidine), activity of the antioxidant enzymes glutathione peroxidase (GPx) and superoxide dismutase (SOD), measurement of lipid peroxidation (TBARS) and protein oxidation. A decrease in sperm motility parameters was observed after 72 hr of cooling (i.e. total and progressive) with a higher percentage of minor (37.7 ± 6.3%) and total defects (53.4 ± 6.3%). Additionally, a decrease in high mitochondrial activity (Class I: 16.6 ± 2.2%) occurred after 72 hr. The decrease in motility rates after a long cooling time probably was caused by the increase in sperm abnormalities. A long cooling time causes cold shock and mitochondrial exhaustion, but there was no observed change with the oxidative stress condition. Therefore, cat epididymal sperm stored at 5°C appear to maintain a high quality for up to 48 hr of cooling time.     

Pregnancy‐Induced ISG‐15 and MX‐1 Gene Expression is Detected in the Liver of Holstein–Friesian Heifers During Late Peri‐Implantation Period

The bovine embryonic signal interferon‐τ (IFN‐τ) produced by the trophoblast is known to pass through the uterine fluid towards the endometrium and further into the maternal blood, where IFN‐τ induces specific expression of interferon‐stimulated gene expression (ISG), for example in peripheral leucocytes. In sheep, it was shown experimentally by administration of IFN‐τ that ISG is also detectable in the liver. The objective was to test whether ISG can be detected in liver biopsy specimens from Holstein–Friesian heifers during early pregnancy. Liver biopsies were taken on day 18 from pregnant and non‐pregnant heifers (n = 19), and the interferon‐stimulated protein 15 kDa (ISG‐15) and myxovirus‐resistance protein‐1 (MX‐1) gene expression was detected. The expression of both MX‐1 (p: 24.33 ± 7.40 vs np: 9.00 ± 4.02) and ISG‐15 (p: 43.73 ± 23.22 vs 7.83 ± 3.63) was higher in pregnant compared to non‐pregnant heifers (p < 0.05). In conclusion, pregnancy induced ISG‐15 and MX‐1 gene expression in the liver already at day 18 in cattle.     

Isolation of Leptospira noguchii from sheep

The main goal of this study was to obtain new isolates of Leptospira spp. from sheep. A total of 10 kidney samples and 44 blood samples were collected from sheep slaughtered in Pelotas, Southern Brazil. One isolate was obtained which was identified by 16S rRNA gene sequencing and serogrouping to be Leptospira noguchii serogroup Autumnalis. Microscopic agglutination test (MAT) evaluation revealed that 4.5% of the sheep sera reacted against the Autumnalis serogroup. This is the first report of isolation of L. noguchii from sheep. Together these findings indicate that L. noguchii infections may be a potentially important veterinary problem in this domestic animal species.     

Contrasting edge and pasture matrix effects on ant diversity from fragmented landscapes across multiple spatial scales

Landscape Ecology - Harmful effects of habitat loss and fragmentation can be detected across multiple spatial scales, yet most studies that aim to characterize these effects take place at a single...     

Establishment techniques affect productivity,nutritive value and atmospheric N2 fixation of two sunn hemp cultivars

Nitrogen fertilization is a common practice for sustaining forage production in forage systems in southeastern United States. Warm-season annual legumes may be an alternative forage to warm-season perennial grasses that do not require N fertilization. Sunn hemp (Crotalaria juncea L.) is a fast-growing, warm-season annual legume native to India and Pakistan. The objective of this 2-year study was to assess the herbage accumulation (HA), atmospheric N₂ fixation (ANF) and nutritive value of sunn hemp. Treatments were the factorial arrangement of two sunn hemp cultivars (“Crescent Sun” and “Blue Leaf”), three seeding rates (17, 28 and 39 kg seed/ha) and seed inoculation (inoculated or non-inoculated seeds), distributed in a randomized complete block design with four replicates. Crescent sun had greater HA (3,218 vs. 1764 kg DM/ha) and ANF (41 vs. 25 kg N/ha). Blue leaf had greater crude protein (CP) (188 vs. 176 g/kg) and in vitro digestible organic matter (IVDOM) concentrations (564 vs. 531 g/kg) than crescent sun. Non-inoculated seed had greater CP than inoculated seed, 188 and 177 g/kg, respectively, and inoculation did not affect HA. Intermediate seeding rate (28 kg/ha) decreased HA (2002 kg DM/ha), while HA from high and low seeding rates (17 and 39 kg/ha, respectively) did not differ (2,863 and 2,615 kg DM/ha respectively). Planting non-inoculated crescent sun at 17 kg/ha seeding rate is a feasible management practice to produce sunn hemp in subtropical regions; however, inoculation should always be recommended for proper establishment.     

Fall Armyworm Damaged Maize Plant Identification using Digital Images

The objectives of precision agriculture are profit maximisation, agricultural input rationalisation and environmental damage reduction, by adjusting the agricultural practices to the site demands. The fall armyworm (Spodoptera frugiperda) is one of the most important maize pests in Brazil and the use of insecticide is the main control method. It is believed that site-specific control can be implemented by using a machine vision system. The objective of this work was to develop and evaluate an algorithm at simplified lighting conditions for identifying damaged maize plants by the fall armyworm using digital colour images. Images of damaged and non-damaged maize plants were taken in eight different stages and in three different light intensities. The proposed algorithm had two stages: the processing and the image analysis. During the first stage, the images were processed to create binary images where the leaves were segmented from the other pixels. At the second stage, the images were subdivided into blocks and classified as ‘damaged’ or ‘non-damaged’ depending on the number of objects found in each block. The algorithm correctly classified 94·72% of 720 images.     

Disseminated pythiosis in three horses

Three cases of equine subcutaneous pythiosis with dissemination to the internal organs were investigated. The subcutaneous lesions were observed on the mammary gland, nostrils and limbs of the infected horses. Histopathological analysis of the infected tissues revealed a strong eosinophilic reaction, with macrophages, mast cells and giant cells. Sparsely septated hyphal filaments of 4-6 microm diameter were identified in the center of the eosinophilic areas. Specific fluorescent antibody against Pythium insidiosum confirmed the hyphae in the infected tissues in three examined horses. In one of the three cases, the DNA sequences amplified from the infected subcutaneous tissues and internal organs, revealed that P. insidiosum's 18S SSU rDNA amplicons shared 100% identity with those sequences deposit in GenBank. This is the first report confirming by immunochemical and genetic techniques that P. insidiosum can disseminated from superficial to deep structures.