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Intramammary infusion of an Enterococcus faecium SF68 preparation promoted the involution of drying off Holstein cows partly related to neutrophil‐associated matrix metalloproteinase 9 下载免费PDF全文
Attapol Tiantong Hsing‐Yi Peng Shuen‐Ei Chen Piya Piamya Wen‐Bor Liu Ming‐Tsao Chen Chi Yu Hajime Nagahata Chai‐Ju Chang 《Animal Science Journal》2015,86(1):111-119
A problem for dairy cows following milk stasis is to cope with a high risk of intramammary infection and there is a need to initiate an extensive renewal of secretory modules in mammary glands so that milk production in next lactation may be optimized. We recently reported that ultrasonicated Enterococcus faecium SF68 (SF68) is compatible with cow mammary glands and an enhancer of innate immunity during the immediate post‐milk stasis period. The current study further examines the concomitant effect of ultrasonicated SF68 on mammary tissue remodeling. Four Holstein cows each received intramammary infusions of regular antibiotic dry‐cow formula (positive control) and two different doses of SF68 in different quarters. Analyses of individual quarter secretion samples showed faster neutrophil infiltration, earlier modifications in protein composition, including caseins and lactoferrins, as well as more prompt elevation of the specific unit of 92‐kDa matrix metalloproteinase 9 (MMP9) in SF68‐infused quarters compared to the positive controls. Intramammary infusion of ultrasonicated SF68 seems able to accelerate the regression of mammary synthetic capacity and potentiate the breakdown of glandular extracellular matrix, indicating a more efficient mammary gland involution. Correlation analyses imply that the ability of ultrasonicated SF68 to induce faster neutrophil chemotaxis and the associated MMP9 release is partly responsible. 相似文献
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Em-on OLANRATMANEE Piya WONGYANIN Roongroje THANAWONGNUWECH Padet TUMMARUK 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1071-1077
The objective of the present study was to investigate the prevalence of porcine
reproductive and respiratory syndrome (PRRS) virus detection in aborted fetuses (n=32),
mummified fetuses (n=30) and stillborn piglets (n=27) from 10 swine herds in Thailand
using quantitative polymerase chain reaction (qPCR). Pooled organs and umbilical cord from
each fetus/piglet were homogenized and subjected to RNA extraction and cDNA synthesis. The
qPCR was carried out on the ORF7 of the PRRS viral genome using fluorogenic probes for
amplified product detection. The results revealed that 67.4% (60/89) of the specimens
contained PRRS virus. The virus was found in 65.6% (21/32) of aborted fetuses, 63.3%
(19/30) of mummified fetuses and 74.1% (20/27) of stillborn piglets
(P=0.664). Genotype 1, genotype 2 and mixed genotypes of PRRS virus were
detected in 19.1% (17/89), 25.8% (23/89) and 22.5% (20/89) of the specimens, respectively
(P=0.316). PRRS virus antigen was retrieved from both
non-PRRS-vaccinated herds (68.2%, 45/66) and PRRS-vaccinated herds (65.2%, 15/23)
(P=0.794). These findings indicated that these specimens are important
sources of the PRRS viral load and the viral shedding within the herd. Thus, intensive
care on the routine management of dead fetuses and stillborn piglets in PRRS
virus-positive herds should be emphasized. 相似文献
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Yu TC Chang CJ Ho CH Peh HC Chen SE Liu WB Peng HY Piamya P Chen MT Nagahata H 《Veterinary immunology and immunopathology》2011,144(3-4):210-219
An appropriate length of milk stasis between two consecutive lactations of dairy cows is crucial for sustainable milk production. This dry period of cows allows extensive remodeling and sufficient cell renewal in mammary gland. Nevertheless, early dry period is one of the most risky stages in cow lactation cycle to intramammary infection. Dry-cow treatment through teats is, therefore, widely practiced at the commencement of milk stasis. Neutrophils are the most abundant cellular components in cow mammary secretion during early dry period, which in turn attribute to the meanwhile elevation of somatic cell counts and matrix metalloproteinase-9 (MMP-9) level. This study used bovine peripheral neutrophils as a cell model to examine the mode of modifications in their defense and remodeling functionalities after infiltration into mammary gland during early dry period. Results indicate a dose-dependent suppression of phorbol 12-myristate 13-acetate-stimulated free radical production and induction of MMP 9 degranulation in bovine peripheral neutrophils exposure to the d 7-dry secretion of cows received dry cow treatment at d 0 in milk stasis. Meanwhile, an enhancement of plasminogen activation and TNF-α shedding on bovine peripheral neutrophils were also observed. These two cellular events might be involved in the functional modifications on infiltrated neutrophils during early dry period. In conclusion, the opposite trend of modifications in the defense and matrix remodeling functionalities of neutrophils inside the mammary gland of cows at early dry period reflect the collaboration of infiltrated neutrophils for promoting extensive glandular remodeling at minimum compromise of local defense during the acute involution period without apparent disturbance by dry cow treatment. 相似文献
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