Biological Control of Yellow Nutsedge with the Indigenous Rust Fungus Puccinia canaliculata

Yellow nutsedge (Cyperus esculentus L.) is a serious weed problem in the United States and other countries. An indigenous rust fungus [Puccinia canaliculata (Schw.) Lagerh.], pathogenic on yellow nutsedge, was released in early spring as a potential biological control agent. The fungus inhibited nutsedge flowering and new tuber formation. The fungus also dehydrated and killed nutsedge plants. The successful control of yellow nutsedge by a rust epiphytotic under experimental conditions demonstrates the potential use of the rust in an integrated weed management system.     

Plant density studies with direct seeded tomatoes in Ontario, Canada

In direct seeded tomato density experiments with constant rectangularity of 1.0 carried out at Preston and Simcoe Research Stations, Ontario, Canada in 1971, the effect of increasing plant density from 4.2 to 62.1 plants/m² was to increase total plant weight, total fruit weight and ripe fruit weight per unit area. The proportion of ripe fruit to total fruit increased with increased density, and ripe fruit yields from a single harvest of up to 14 kg/m² were obtained.Using the reciprocal yield-density equation (when W is the mean weight per plant (or plant part) at density , A and B are constants, and density is the only variable) to analyse the experiments, showed that both the A and B constants were influenced by cultivar while the A constant was influenced more by fertilizer application than was the B constant. In general the effect of increased fertilizer was to increase the yield potential ( ) for total plant weight and total fruit weight, but this effect was not so marked for ripe fruit weight.     

Canopy photosynthesis,stomatal conductance and yield ofSolanum tuberosum grown in a warm climate

Selected potato (Solanum tuberosum L.) genotypes were grown in the field from May to September during 1984 and 1985, to study their growth and gas exchange responses in a warm climate. The parameters measured were leaf area index (LAI), canopy photosynthesis on ground area basis (CPn-Ga), stomatal conductance, dry matter partitioning and yield. The LAI ranged from 1.40 for Dakchip to 6.60 for Pungo during August 1984. The LAI also differed significantly among the potato genotypes for the three samplings during 1985. Atlantic, Chipbelle and DTO-33 showed no decline in their LAI up to 73 days after planting (DAP), indicating a better heat-stress tolerance response than the other genotypes. During both years, CPn-Ga differed significantly among the genotypes and Pungo had higher CPn-Ga than all the other genotypes. Mean CPn-Ga rates were 1.72 and 4.34 g CO₂ m^?2hr^?1 during 1984 and 1985, respectively. Mean adaxial and abaxial stomatal conductances were 0.86 and 1.46 cm sec^?1, during 1984, and stomatal conductances were similar for both years. Stomatal conductance did not appear to limit gas exchange in potato leaves. Dry matter partitioning to tubers ranged from 8.9% for Pungo to 55.5% for Atlantic 67 DAP during 1984. At final harvest, July 19, 1985, dry matter partitioning to tubers varied from 47.5% for Pungo to 69.9% for Chipbelle. The tuber yield ranged from 9.6 to 27.8 MT/ha. This study indicated that Atlantic and La Chipper have potential for growing in a warm climate.     

Nitrapyrin,terrazole, atrazine and simazine influence on denitrification and corn growth

In modern agriculture, long‐term soil fertility and crop productivity are maintained by a combination of inorganic fertilizers and pesticide inputs which, in turn, create environmental and health concerns. Therefore, studies were initiated to evaluate two commonly used herbicides (atrazine and simazine) and two biological nitrification inhibitors (nitrapyrin and terrazole) applied with NO₃‐N source fertilizer for their effects on denitrification and on corn (Zea mays L.) growth and yields. Each chemical applied at the rate of 10, 50, or 100 mg a.i. L^‐1 suppressed denitrification of NO₃ ^‐ in a liquid medium inoculated with a Tifton loamy sand in a laboratory study. Nitrapyrin and terrazole selectively suppressed NO₃ ^‐ or NO₂ ^‐ or both reduction while atrazine and simazine suppressed NO₂ ^‐ or N₂O or both reduction. In greenhouse pot culture studies, chemical application resulted in higher percent N recovery relative to the control. When atrazine or simazine was part of the chemical treatment, concentrations of NO₃ ^‐ and NO₂ ^‐ in corn (Zea mays L.) plants increased, and plant growth was restricted due to NO₂ ^‐ toxicity. During two consecutive years of field studies using split‐banded applications of N fertilization with nitrapyrin and terrazole, corn ear yields increased 78% and 25% in the first and second year, respectively. With atrazine and simazine, however, yields increased significantly in the first season only. Mixing either herbicide with nitrapyrin or terrazole had no effect on yields during both seasons.

Chemical Names: atrazine = [2‐chloro‐4‐ethylamino‐6‐isopropylamino‐s‐triazine]; simazine = [2‐chloro‐4,6‐bis(ethylamino)‐s‐triazine]; nitrapyrin = [2‐chloro‐6‐(trichloromethyl)pyridine]; terrazole = [ethoxy‐3‐trichloromethyl‐1,2,4‐thiadiazole].     

Organogenesis and terpenoid synthesis in Mentha arvensis

Leaf discs obtained from field grown plants of Mentha arvensis were used to initiate multiple shoots on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (5 mg l(-1)) and naphthaleneacetic acid (0.5 mg l(-1)). Profuse rooting was achieved when the well-grown shoots were cultured on half strength MS medium supplemented with indole-3-acetic acid (2 mg l(-1)). The regenerated plantlets were hardened and successfully transferred to soil and grown to maturity. Tissues at different stages of differentiation were analyzed for their essential oil content and characteristic monoterpene pattern. Tissue culture raised plants show the same essential oil profile as that of the parent plant. However, tissues at early stages of growth show distinct changes in oil composition, such as high levels of pulegone in shoot cultures.     

Growth and elemental composition of tomato as affected by fungicides and nitrogen sources

Tomato (Lycopersicon esculentum Mill.) plant growth and elemental composition were evaluated using three NH₄‐N:NO₃‐N form ratios with or without the fungicide benomyl, captan, lime‐sulfur, nitrapyrin, or terrazole in a greenhouse soil culture study. Nitrogen was applied weekly for 5 weeks providing a total of 115 mg N/kg. Each fungicide was applied at 0.25 mg/kg 3 days before transplanting, followed by 3 weekly applications with each N treatment. The largest shoot and root dry weights were obtained with 1:1 N form ratio. With 1:1 N ratio treatment, all chemicals significantly increased plant growth resulting in lower element concentrations relative to the untreated control. However, growth of plants receiving either 1:0 or 0:1 N ratio treatment was not affected by nitrapyrin and terrazole, but was restricted by benomyl, captan, or lime‐sulfur. Overall, elemental concentrations in the tissues of plants receiving either N form was related to the fungicide treatment.     

Influence of biocides on tomato nitrogen uptake and soil nitrification and denitirification

As a result of repeated applications, some fungicides may accumulate in the soil to levels high enough to have adverse effects on the activity of soil microorganisms and plant growth. Comparison of the effects of 10 mg kg^‐1 soil of the benlate, captan, and lime‐sulfur fungicides with the nitrification inhibitors (NI) nitrapyrin and terrazole on oxidation of NH₄ ⁺ in Tifton loamy sand (siliceous, thermic plinthic Typic Kandiudults) incubated at 30° C showed that benlate had no significant effects whereas captan inhibited nitrification 21% more than lime‐sulfur, but about 20% less than NI. Application of benlate enhanced NO₃ ^‐ reduction to N₂O and N₂ in liquid medium inoculated with soil whereas 50 and 100 mg L"¹ medium of captan and lime‐sulfur compared favorably with the NI in suppressing NO₃ ^‐ and NO₂ ^‐ reductions, but were less effective than the inhibitors when applied at the low rate of 10 mg L^‐1 medium. In a greenhouse study with tomato (Lycopersicon esculentum Mill. cv. ‘Better Boy'), weekly drench applications of 0.25 mg kg^‐1 soil of the test biocides for four weeks with three NH₄ ⁺‐N: NO₃ ^‐‐N ratios showed that benlate applied with 1: 0 N ratio and lime‐sulfur applied with 0: 1 N ratio restricted significantly the plant growth and N uptake. The largest root: shoot ratios, total plant dry weight, and N uptake were obtained with plants fertilized with 1: 1 N ratio in combination with the biocides.     

Relative resistance of potato cultivars to bacterial wilt

Fifty-one potato cultivars were evaluated for reaction to infection byPseudomonas solanacearum Sm. under high disease pressure in a field plot near Tifton, Georgia during the spring of 1978 and 1979. Plots were infested by clipping beds of thickly seeded tomato plants with a rotary mower contaminated with the wilt baterium in 1975, 1976, 1977 and 1978. The plants were incorporated into the soil following disease development. In addition to the use of infested soil, one stem on each potato plant was inoculated in late May by cutting with a knife blade dipped in a bacterial suspension. Most of the cultivars evaluated were highly susceptible to bacterial wilt. Ontario was the only cultivar highly resistant to the disease, with only 1% and 8% dead or dying plants in 1978 and 1979, respectively. Based on tuber infection, Ontario and Snowchip were the most resistant, with 9% and 14% infection, respectively, in 1978 and both 4% in 1979. Saco, Green Mountain and Sebago also showed some resistance.     

Engineering needs for potato production from true seed

Potatoes grown from true potato seed (TPS) are generally small and nonuniform in size and shape, compared to the ones produced conventionally from seed tubers. Potato production from TPS would be considerably less expensive than from seed tubers because of the negligible storage and handling costs of the TPS. Potato production from TPS would therefore have the greatest potential in the developing countries. In developed countries, this production method would have some potential in commercial production as well as in home gardening. Research related to the TPS potato production has been mostly directed toward investigating suitable cultural practices, screening breeding lines for higher yield and warm climate adaptability, weed control in the field, and other similar production areas. Research concerning mechanization of TPS potato production has been limited. Engineering input to the TPS potato production, handling and storage needs considerable attention to develop practical and economical practices. This paper highlights some research areas which the authors believe are worth investigating. Engineering research that is conducted at the Coastal Plain Experiment Station related to the TPS potato production has been outlined along with future plans.