Seasonal Changes in the Milt Quality of Waigieu Seaperch,Psammoperca waigiensis: Implications for Artificial Propagation

Milt quality of Waigieu seaperch was sampled at the beginning, the middle, and the end of the spawning season to assess if the sampling season has an effect on milt quality parameters. The milt volume and total sperm production were higher at the middle of the spawning season while the sperm concentration in milt was significantly higher at the beginning and the end of the spawning season. Sperm morphology and the major parameters of seminal plasma (pH, total protein, Mg²⁺, or Ca²⁺ concentrations) did not differ throughout the spawning season. The concentrations of Na⁺ and the osmolality increased throughout the spawning season. The percentage of motile cells did not differ during the spawning season while the duration of sperm motility (swimming duration) and velocity (swimming speed) were significantly different during the spawning season. The fertilization rate (75.7 ± 6.8%) and hatching rate (56.5 ± 3.1%) at the middle of the spawning season were higher than at the beginning and end periods. Results indicated that milt quality parameters and fertility success in the middle of the spawning season were higher compared to earlier and later sampling dates. Thus, we recommend collecting milt during this time for maximal fertilization and hatching success.     

中国平背树螽属两新种(直翅目:螽斯科:树螽亚科)

描述了平背树螽属2新种,定名为双刺平背树螽(Isopsera bicuspidota Yang etKang)和广西平背树螽(I.guangziensis Yang et Kang)。     

17β-雌二醇-ARIS构建及其标准曲线的质量评估

以 1 7β-雌二醇 -黄素腺嘌呤二核苷酸结合物为标记物 ,以葡萄糖氧化酶为全酶 ,建立了 1 7β-雌二醇脱辅基酶再激活免疫测定系统 (1 7β- E2 - ARIS) ,完成了两条标准曲线的制作 ,通过 logit代换法对两条标准曲线进行了直线回归。两条标准曲线的拟合度 (r)分别为0 .996 7* * 和 0 .9991 * * ,灵敏度分别为 1 1 .5 4pg· m L- 1 (2 . 31 pg·孔 - 1 )和 1 4.88pg·m L- 1 (2 . 98pg·孔 - 1 ) ,平均变异系数(CV% )分别为 4.6 4%和 9.34% ,测定范围为 31 .2 5～ 1 0 0 0 pg·m L- 1 。     

江西省古树资源系统研究报告

研究对象只限于散生于江西省山下及村落的30851株古树，不涉及自然保护区、森林公园中的古树。根据古树生长特征，分析其时间分布(古树级及树龄)、空间分布(设区市分布、县域分布)，借助于动态聚类统计分析方法从不同方向进行数量分类，并对数量分类成果用判别分析进行检验。最后对各县域每km^2和每万人负荷古树数进行核算，用数据资抖说明县域保护古树的重要性，增强县域保护古树的责任感。     

17β─雌二醇主动免疫对公兔生殖的影响

以１７β－雌二醇－６－人血清白蛋白对新西兰公兔进行主动免疫，用ＲＩＡ检测抗体滴度和１７β－雌二醇及睾酮浓度，并检测睾丸重量和间质细胞面积。结果，试验组各兔均不同程度地产生了１７β－雌二醇抗体，其血浆１７β－雌二醇浓度极显著地低于对照组〔（２０．３±２１．６）ｎｇ／Ｌ，（１６７．７±４９．６）ｎｇ／Ｌ，Ｐ＜０．００１〕，睾酮浓度极显著地高于对照组〔（７．３±７．１）μｇ／Ｌ，（０．５２±０．２９）μｇ／Ｌ，Ｐ＜０．０１〕。每侧睾丸重量和间质细胞面积均极显著地大于对照组〔（３．９４±０．９３）ｇ和（２５２．２５±８５．７８）μｍ２，（２．９８±０．７２）ｇ和（１６１．７９±３６．４５）μｍ２，Ｐ＜０．０１和Ｐ＜０．００１〕。提示１７β－雌二醇主动免疫可望成为提高雄性动物生育力的有效途径之一     

FACTORS AFFECTING THE LOSS OF TRI-ALLATE FROM SOILS

Summary. Electron-capture gas chromatography was used to detect tri-allate residues in persistence studies with two soils. At rates equivalent to 0–75, 15 and 3 Ib/ac, 50% of the amount applied was degraded in 8–11 weeks at 25°C in moist Regina heavy clay and Weyburn loam. No loss occurred in sterile soils, indicating that microbial degradation may be a, major factor contributing to tri-allate breakdown.
When aqueous solutions buffered at pH 4–8 were held at 25° G, only 10–15% of the tri-allate was chemically degraded during 24 weeks.
At the normal field rate of 1·25 Ib/ac, tri-allate was not readily leached. From soil columns of Weyburn loam, 5–7% was eluted by 9 in. of water; with clay the corresponding value was 12–13% of the amount applied. When field plots were sprayed with 125 lb/ac in April, tri-allate could still be detected until the soil froze in November.
Facteurs agissant sur la perte de tri-allate dans les sots     

Tests of soil fertility following repeated applications of MCPA, tri-allate, simazine and linuron

The following herbicides were applied annually from 1963 to experimental plots of appropriate crops grown in monoculture: MCPA 1.7 kg ha^?1, triallate 1.7 kg ha^?1, simazine 1.7 kg ha^?1 and linuron 0.84 kg ha^?1 (applied twice per year). Before the eighth treatment in 1970, nutrient status, pH and growth in greenhouse tests of a range of plants were similar in soils from treated and control plots. There were no significant differences in yield when several test crops were grown in the field plots in 1977. In a similar experiment which ran for 6 years, the same herbicides were applied twice per year at twice the above rates on each occasion (three times a year at 1.7 kg ha^?1 in the case of linuron) to uncropped plots. Three years after the last treatment, there were no differences in extractable nutrients, pH, soil structure and crop yield on treated or control plots. These results support the conclusion from the main monitoring of the experiments reported elsewhere that annual treatments with these herbicides have had no adverse affect on the soil.     

Determination of extractable and non-extractable radioactivity from prairie soils treated with carboxyl- and ring-labelled [14C]2,4-D

Ring- and carboxyl-labelled [¹⁴C]2,4-D were incubated under laboratory conditions, at the 2 g/g level, in a heavy clay, sandy loam, and clay loam at 85% of field capacity and 20 1C. The soils were extracted at regular intervals for 35 days with aqaeous acidic acetonitrile, and analysed for [¹⁴C]2,4-D and possible radioactive degradation products. Following solvent extraction, a portion of the soil residues were combusted in oxygen to determine unextracted radioactivity as [¹⁴C]carbon dioxide. The remaining soil residues were then treated with aqueous sodium hydroxide, and the radioactivity associated with the fulvic and humic soil components determined. In all soils there was a rapid decrease in the amounts of extractable radioacitivity, with only 5% of that applied being recoverable after 35 days. All recoverable radioactivity was attributable to [¹⁴C]2,4-D, and no [¹⁴C]-containing degradation products were observed. This loss of extractable radioactivity was accompanied by an increase in non-extractable radioactivity. Approximately 15% of the applied radioactivity, derived from carboxyl-labelled [¹⁴C]2,4-D, and 30% from the ring-labelled [¹⁴C]2,4-D was associated with the soil in a non-extractable form, after 35 days of incubation. After 35 days, less than 5% of the radioactivity from the carboxyl-labelled herbicide, and less than 10% of the ringlabelled material, was associated with the fulvic components derived from the three soils. Less than 5% of the applied radioactivities were identifiable with any of the humic acid components. It was considered that during the incubation [¹⁴C]2,4-D did not become bound or conjugated to soil components, and that non-extractable radioactivity associated with the three soil types resulted from incorporation of radioactive degradation products, such as [¹⁴C]carbon dioxide, into soil organic matter.     

Field experiments to investigate long-term effects of repeated applications of MCPA, tri-allate, simazine and linuron: II. Crop performance and residues 1969–78

In four field experiments begun in 1963, each of four herbicides was applied to plots planted wilh the same crop each year. The annual treatments were: MCPA at 17 kg/ha to barley (Hordeum sativa Jess) and wheat (Triticum aestivum L.) at growth stage 15. tri-allate at 17 kg/ha pre-emergcnce to barley and wheat, simazine at l7kg/ha pre-emergence to maize (Zea mays L.) and linuron in two applications of 084 kg/ha pre- and post-emergence to carrots (Daucus carota L.). MCPA did not affect growth or yield of either barley or wheat. In general tri-allate also did not aftect the crops although wheat yield was depressed in 1978, wheat 1000 grain weight was reduced in 1972 and barley germination percentage was increased in 1973. Simazine did not influence the height, yield or appearance of maize. Linuron normally produced no effect on carrot yield, density and size. However, in 2 years when the post-emergence application was late, density but not yield was lower than in control plots. There was no accumulation of residues of any of these compounds in the soil. Rates of loss were similar to those predicted on ihe basis of laboratory experiments. In a fifth experiment these herbicides were applied twice per year (3 times in the case of linuron) at double the rales above on each occasion to bare plots. These applications ceased in 1968 (1969 for MCPA) but residues were monitored until 1972 except in the case of MCPA. Disappearance rates were similar to those in the cropped plots and residues were largely confined to the top 10 cm. The plots treated with MCPA had developed an enhanced ability lo degrade il prior to 1968. This persisted for 5 years after the final application.