排序方式: 共有48条查询结果,搜索用时 265 毫秒
1.
Chie FUJISAWA Noriko INADA Rika MOCHIZUKI Katsuhiko ARAI Kohkichi UEHARA 《Animal Science Journal》2003,74(3):223-229
Matrix metalloproteinases 2 and 9 (MMP‐2 and ‐9) are zinc‐dependent metalloenzymes and have gelatin‐degrading activity. Both MMP are known to be secreted by many types of cells and play important roles in several biological changes including tissue remodeling and wound healing. In the present study, a primary culture of murine epidermal keratinocytes was prepared and effects of transforming growth factor‐β (TGF‐β), tumor necrosis factor‐α (TNF‐α) and interferon‐γ (IFN‐γ) on expression of MMP‐2 and MMP‐9 by the keratinocytes was examined. Gelatin zymography revealed that murine epidermal keratinocytes secreted proenzyme forms of MMP‐2 and MMP‐9, but the active forms of both MMP were hardly detectable, indicating that in vitro autoactivation of these proenzymes did not occur. Both TGF‐β and TNF‐α stimulated MMP‐9 production in a dose‐dependent manner, but the MMP‐2 level was not changed. Interferon‐γ hardly affected production of MMP‐2 or MMP‐9. Ribonuclease protection assay demonstrated that TNF‐α increased the level of MMP‐9 mRNA 6‐fold compared to the control, whereas TGF‐β slightly up‐regulated it. These results suggest that expression of MMP‐9 could be regulated by several cytokines in murine epidermal keratinocytes. 相似文献
2.
Thyroid hormone deficiency in abnormal larvae of the Japanese flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis> 总被引:1,自引:0,他引:1
Nozomi?OkadaEmail author Tetsuo?Morita Masaru?Tanaka Masatomo?Tagawa 《Fisheries Science》2005,71(1):107-114
ABSTRACT: In large-scale rearing of juveniles of the Japanese flounder Paralichthys olivaceus, a certain morphological abnormality occurred spontaneously in 4% of the fish. These fish showed a slight but clearly different appearance from any developmental stage of this species, and did not settle when all the other juveniles in the same tank completed metamorphosis and had settled. From comparisons of external and internal structures between the normal and the abnormal fish, the abnormality was attributed to unbalanced progress of metamorphosis, mainly due to metamorphic stasis. The thyroid of the abnormal fish was apparently activated morphologically. In addition, serum thyroxine (T4) concentrations in the abnormal fish were reduced to less than 1/10 of that of normal fish. After 14 days of T4 treatment (0.1 p.p.m) of the abnormal fish, all the abnormal characteristics disappeared, and the fish recovered to normal, suggesting normal responsiveness to thyroid hormones in peripheral tissues, whereas thiourea treatment (30 p.p.m., 14 days) further delayed metamorphosis. These results suggest that these abnormal fish were suffering from thyroid hormone deficiency, and were unable to secrete a sufficient amount of thyroid hormone to complete metamorphosis. 相似文献
3.
Effects of a rice diet and phytase addition on growth performance,tissue weights,phosphorus and nitrogen retention,and on liver threonine dehydrogenase,malic enzyme and fatty acid synthase activities in broiler chicks 下载免费PDF全文
This experiment was conducted to clarify the nutritional functions of rice and phytase addition for broiler chicks. Thirty‐six 7‐day‐old male chicks (ROSS 308 strain) were assigned to one of the four treatment groups: corn‐ or rice‐based diet groups and each diet with added phytase (2000 phytase units/kg diet) groups (corn + P or rice + P groups). The non‐phytate phosphorus (npP) content in the diets with added phytase was approximately half of the requirement. Body weight gain and feed intake in the rice group was significantly higher than those in the corn group. Breast and thigh muscle weights and nitrogen retention in the rice group were significantly higher than that in the corn group. Although the efficiency of phosphorus retention (%) in the corn + P group was significantly higher than that in the corn group, no significant difference was observed between the rice and rice + P groups. Liver threonine dehydrogenase activity in the corn group was significantly higher than in the other three groups. These results indicate that rice is superior to corn as a starter diet in broiler chicks, and that phytase action in the rice‐based diet was less than that in the corn‐based diet. 相似文献
4.
Masanao Yokohira Yuko Nakano Nozomi Hashimoto Keiko Yamakawa Fumiko Ninomiya Sosuke Kishi Kousuke Saoo Katsumi Imaida 《Journal of toxicologic pathology》2012,25(4):257-263
In vivo, nicotine in cigarette smoke induces various effects not only on the
respiratory system but also the central and peripheral nerve systems, circulatory organs and
digestive organs, and there is a possibility of promotion of lung tumorigenesis. The present
experiment was conducted to examine histopathological changes caused by nicotine in the lung
with repeated intratracheal instillation (i.t.). Six-week-old male F344 rats were administered
nicotine by i.t. at doses of 0.05, 0.1 and 0.2 mg nicotine/rat every 3 weeks beginning at week
4, for up to a total of 9 times and were then sacrificed at week 30. The total number of
administrations, total dose of nicotine and effective number of rats were 9 times, 0.45 mg and
5 rats and 4 times, 0.20 mg and 5 rats for the 0.05 mg nicotine/rat group; 3 times, 0.30 mg and
5 rats and 4 times, 0.40 mg and 3 rats for the 0.1 mg group; and 3 times, 0.60 mg and 3 rats
for the 0.2 mg group, respectively. As a control group, 5 rats were administered 0.2 ml
saline/rat 9 times. Some rats administered 0.1 and 0.2 mg nicotine suffered convulsions just
after administration. Histopathologically, though proliferative changes were not observed,
neutrophil infiltration, edema and fibrosis in the lung were induced by nicotine. In
conclusion, repeated treatment of nicotine promoted neurologic symptoms in the acute phase, and
strong inflammation in the lungs in the chronic phase, even at a low dose. Toxicity of nicotine
is suggested to depend not on total dose of nicotine in the experiment but rather on repeated
injury with consecutive administration. 相似文献
5.
Yokohira M Kuno T Yamakawa K Hashimoto N Ninomiya F Suzuki S Saoo K Imaida K 《Journal of toxicologic pathology》2009,22(1):1-10
It is an urgent priority to establish in vivo bioassays for detection of hazards related to fine particles, which can be inhaled into deep lung tissue by humans. In order to establish an appropriate bioassay for detection of lung damage after particle inhalation, several experiments were performed in rats using quartz as a typical lung toxic particle. The results of pilot experiments suggest that Days 1 and 28 after intratracheal instillation of 2 mg of fine test particles in vehicle are most appropriate for detection of acute and subacute inflammatory changes, respectively. Furthermore, the BrdU incorporation on Day 1 and the iNOS level on Day 28 proved to be suitable end-point markers for this purpose. An examination of the toxicity of a series of particles was performed with the developed bioassay. Although some materials, including nanoparticles, demonstrated toxicity that was too strong for sensitive assessment, a ranking order could be clarified. The bioassay thus appears suitable for rapid hazard identification with a possible ranking of the toxicity of various particles at single concentrations. 相似文献
6.
Fisheries Science - We examined phytoplankton productivity in Shido Bay, an active oyster farming area in the eastern Seto Inland Sea, Japan, by an in situ 13C method from March 2016 to January... 相似文献
7.
Daisuke Hattori Tanaka Kenzo Nozomi Yamauchi Kazuo Okamura Irino Joseph Jawa Kendawang Ikuo Ninomiya 《Soil Science and Plant Nutrition》2013,59(2):218-228
Enrichment planting with climax trees such as dipterocarps is required to rehabilitate degraded tropical forests in Southeast Asia, because these forests have been damaged by various anthropogenic activities. Severely degraded tropical secondary forests are the most important targets for enrichment planting in the region, because secondary forest trees potentially provide moderate conditions for planted seedlings by preventing strong sunlight and high temperatures. In an enrichment planting site located in Sarawak, Malaysia, we explored how variation in secondary forest structure (biomass and stand density) and soil (nutrient and water) conditions influenced the mortality and growth rates of planted dipterocarp seedlings (Parashorea macrophylla, Wyatt-Sm. ex P.S. Ashton) over the period from 2000–2007. Experimental plots were set up on different topographical features (slopes and valleys). Using the data collected, we demonstrated the ways in which environmental factors, such as soil nitrogen content, relate to climax tree seedling growth and mortality in degraded tropical secondary forests dominated by the genera Macaranga, Ficus, and Glochidion. Stand density and total aboveground biomass of secondary forest trees were significantly lower in the valley plots than on the slopes, whereas soil total nitrogen and water contents were significantly higher in valley plots. Over seven years, the total biomass of trees increased, whereas the density of secondary forest trees decreased in all plots. Nutrient stocks (nitrogen, phosphorus, potassium, calcium, and magnesium) in the soil (0–5?cm) also decreased over seven years in all plots. Height, diameter, and mortality of planted seedlings were higher in the valley plots than on slopes. Multiple stepwise regression demonstrated significant positive effects of total soil nitrogen and water content on the relative growth rate of seedlings over seven years. There were no significant relationships between seedling mortality and any of the environmental factors measured, including soil conditions. We conclude that (1) seedling growth was enhanced by soil total nitrogen rather than by available phosphorus or potassium, and (2) planted seedlings grew faster and died more often in the valley plots than on the slopes. 相似文献
8.
Sakuma M Nishio T Nakanishi N Izawa M Asari Y Okamura M Shimokawa Miyama T Setoguchi A Endo Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(10):1381-1384
We herein present clinical findings of an Iriomote cat with Hepatozoon felis parasitemia. A male Iriomote cat was captured for ecological analyses three times from January 2010 to January 2011. Although this cat did not show any hematological abnormalities at the time of the first capture, H. felis parasitemia and increased serum creatine kinase levels were detected at the second and third captures. H. felis infection was confirmed by polymerase chain reaction, and amplified 18S ribosomal RNA gene fragments were 100% identical to those of H. felis in leopard cats in Korea. Although the virulence of H. felis in this cat was suggested to be low, this is the first report of an H. felis-infected Iriomote cat with parasitemia. 相似文献
9.
K. Panneerselvam Chigen Tsukamoto Nozomi Honda Akio Kikuchi Jeong‐Dong Lee Seung‐Hwan Yang Gyuhwa Chung 《Plant Breeding》2013,132(1):121-126
Seed saponin composition of 3025 wild soybean (Glycine soja Sieb. and Zucc.) accessions collected from nine regions of Korea was analysed by thin‐layer chromatography to determine its polymorphic variation and geographical distribution and find mutants in saponin components. The saponin composition of seed hypocotyls was primarily divided into seven phenotypes, designated as Aa, Ab, AaBc, AbBc, Aa+α, AaBc+α and AbBc+α. The predominant phenotypes were AaBc (55%), Aa (33%), AaBc+α (7.5%) and Aa+α (3.3%). The frequencies of Ab, AbBc and AbBc+α were very low (0.3‐0.5%). Codominant alleles Sg‐1a and Sg‐1b and dominant allele Sg‐4 occupied 98.6, 1.1 and 63.3%, respectively. Alleles Sg‐3 and Sg‐5 were found to be dominant in all the analysed accessions except the mutants. Three accessions were discovered as mutants via LC‐PDA/MS/MS. The accession CWS0115 did not produce saponin Aa and Ax, CWS2133 did not produce saponin Aa and Ab and CWS5095 did not produce any group A saponins. These newly determined mutants might be utilized in producing a new soybean variety with good taste as well as in biosynthetic studies. 相似文献