Recovery of brodifacoum in vomitus following induction of emesis in dogs that had ingested rodenticide bait

AIM: To assess the benefit of inducing emesis in dogs that have ingested rodenticide bait containing brodifacoum (BDF), by determining the amount of BDF in bait recovered from the vomitus relative to the estimated amount consumed.

METHODS: Between 2014 and 2015 samples of vomitus from seven dogs that ingested rodenticide baits containing BDF were submitted by veterinarians in New Zealand. All seven dogs had been given apomorphine by the veterinarian and vomited within 1 hour of ingesting the bait. Some or all of the bait particles were retrieved from each sample and were analysed for concentrations of BDF using HPLC. Based on estimations of the mass of bait consumed, the concentration of BDF stated on the product label, and the estimated mass of bait in the vomitus of each dog, the amount of BDF in the vomited bait was calculated as a percentage of the amount ingested.

RESULTS: For five dogs an estimation of the mass of bait ingested was provided by the submitting veterinarian. For these dogs the estimated percentage of BDF in the bait retrieved from the vomitus was between 10–77%. All dogs were well after discharge but only one dog returned for further testing. This dog had a normal prothrombin time 3 days after ingestion.

CONCLUSIONS AND CLINICAL RELEVANCE: The induction of emesis within 1 hour of ingestion can be a useful tool in reducing the exposure of dogs to a toxic dose of BDF. The BDF was not fully absorbed within 1 hour of ingestion suggesting that the early induction of emesis can remove bait containing BDF before it can be fully absorbed.     

Veränderungen der Vegetation durch experimentelle Erdgasbehandlung

Changes in vegetation caused by experimental leakage of natural gas The response of vegetation to natural gas leakage was studied in Central Bohemia, Czechoslovakia, in 1978–84. The gas used was of the following chemical composition: 93·8% methane, 3·6% ethane, 0·8% propane, 0·25% butane, 0·3% carbon dioxide. The test crops were planted in 60·5 m plots containing underground bores from which gas was released into the soil through distribution pipes. In addition, the response of some weed species was recorded. The important effects of contamination observed were restricted growth and decrease in the number of individuals present. A change in the green colour of leaves was recorded in Medicago sativa, Brassica oleracea var. acephala, Secale cereale, Lolium multiflorum. Beta vulgaris and Zea mays. An obvious shift of developmental stages was found in Solanum tuberosum. Helianthus annuus and Zea mays (delay of recruitment and blooming). Restricted or even failure of reproduction can be considered the typical effect of exposure to natural gas: this holds true especially for Allium cepa, Triticum aestivum, Hordeum distichon, Trifolium sp.div., Medicago sativa, and most of the weedy species. Various growth deformations, mostly found in the underground parts of the plants (tubers and roots in Solanum tuberosum, Beta vulgaris), can be ascribed to the unfavourable conditions of soil aeration. Plants of Chenopodium album and Medicago sativa growing in the vicinity of the gas release point had an increased number of stomata. There were changes in the course of the reflectance curves estimated in the population of Trifolium pratense subsp. sativum. There was a conspicuous decrease of the near infrared reflectance in the stand exposed to gas in comparison with the control. Decrease of coverage and density of the stands reduced species diversity and changes in the species' composition (reduction of sensitive species) are the general consequences of natural gas leakage. The first symptoms on the vegetation were observed after 15–30 days which corresponds to about 80–150 m³ of discharged gas.     

Sesbania seed collections in Southern Africa

The International Centre for Research in Agroforestry (ICRAF) is a member of the Consultative Group on International Agricultural Research (CGIAR) system and has recently established a Multipurpose Tree Germplasm Resource Unit (MPT-GRU) in Nairobi, Kenya. The first major activity of the MPT-GRU was to undertake a regional germplasm collection ofSesbania in 5 countries in southern Africa in a collaborative mode with National Agricultural Research Services (NARS). The collections took place in Botswana, Malawi, Namibia, Zambia and Zimbabwe.Key elements of a generalised collaborative mode (the model) are indicated follwed by a summary of actual events. The initial phase involved joint planning and training sessions between NARS, three sister CGIAR centres and one regional institution, the Southern Africa Development Conference (SADC) Regional Gene Bank (SRGB). This was followed by actual collections during 1993 led by National scientists with different levels of CGIAR participation in each of the five countries. The International Board of Plant Genetic Resources (IBPGR) took the lead in pre-collection training activities, ICRAF for developing the collection programme and the International Livestock Centre for Africa (ILCA) for seed drying, testing and despatch for field trials. NARS gene banks guided the necessary administrative processes. The ICRAF lead scientist for the species and provenance evaluation phase was a crucial member of the planning process thus allowing integration of the collection with the eventual field evaluation. The article focuses on the collection strategy, administrative protocols for actual collections and summary results. Some advantages and difficulties associated with the collection programme and model are discussed. The collections were important in bringing together agriculturalists and foresters in a common germplasm collection exercise.     

Effects of Addition of Tissue‐Type Plasminogen Activator in In Vitro Fertilization Medium on Bovine Embryo Development and Quality

Plasminogen activators/Plasmin system plays pivotal role in regulating reproductive functions of mammals. Here, we examined the effects of modification of in vitro fertilization medium (IVF medium) with the addition of tissue‐type plasminogen activator (t‐PA), on bovine embryo development and quality, assessed by quantification of expression of various genes related to metabolism, oxidation, implantation and apoptosis. In addition, plasminogen activator activity (PAA) and plasminogen activator inhibition (PAI) were measured in the spent media. After conventional IVM, 2016 cumulus‐oocyte complexes (COCs) were divided into four groups with modified composition of the IVF medium containing t‐PA and/or its inhibitor epsilon‐aminocaproic acid (control, t‐PA, t‐PA+ε‐ACA, ε‐ACA). Presumptive zygotes were cultured for 8 days in synthetic oviductal fluid (SOF) medium; gene expression studies were carried out on morulae and blastocysts. t‐PA alone significantly suppressed cleavage and blastocyst formation rates, but this effect was neutralized by the addition of ε‐ACA. PAA in the treated group was significantly reduced by ε‐ACA, but without total elimination. Significant differences were detected in the expression of genes related to apoptosis and/or cell cycle arrest (BAX, BCL2L1, KAT2B) between embryos produced in t‐PA‐modified media and controls, giving an overall notion that the inferior developmental competence of treated embryos may be attributed to apoptotic phenomena induced by t‐PA. In conclusion, it appears that excessive t‐PA content in the IVF media, suppresses blastocyst formation rate, possibly due to induction of apoptotic phenomena.     

Enzyme-linked immunosorbent assay (ELISA) for the detection of spring viraemia of carp virus (SVCV) in tissue homogenates of the carp, Cyprinus carpio L.

Abstract. An enzyme-linked immunosorbent assay (ELISA) for the demonstration of the virus of spring viraemia of carp (SVCV) in liver, kidney and spleen homogenates, and in infected cell cultures is described. The sensitivity of the method is 10^2·8–10^3·5 TCID₅₀ 0·lml⁻¹ of the examined fluid. The specificity has been confirmed by the ELISA inhibition test and by results of virological examinations. Contamination with bacteria or fungi of samples taken from dead fish had no effect on the results of ELISA. Specific anti-SVCV sera were used successfully for the production of conjugates for the direct immunoperoxidase and immunofluorescence detection of SVCV in infected cell cultures.     

Further evaluation of the use of buparvaquone in the infection and treatment method of immunizing cattle against Theileria parva derived from African buffalo (Syncerus caffer).

Three experiments were undertaken to determine the efficacy of different doses of buparvaquone in the infection and treatment immunization of cattle against Theileria parva derived from African buffalo (Syncerus caffer). Two of these experiments also compared buparvaquone with standard doses of long- and short-acting formulations of oxytetracycline. In addition, different dilutions of stabilates were used in the experiments. In the first experiment, a 10(-1.0) dilution of stabilate was used to infect groups of cattle treated with buparvaquone at doses of between 5 and 0.625 mg kg-1 body weight (bwt) on Day 0 after infection. All control cattle developed severe theileriosis and none of the treatment regimes (including those utilizing long-acting oxytetracycline) prevented the development of theileriosis. Treatment with buparvaquone at 2.5 mg kg-1 bwt or oxytetracycline gave the most satisfactory results. In the second experiment when the sporozoite dose was reduced to 10(-2.0) dilution, buparvaquone treatment at 5 and 2.5 mg kg-1 bwt and short- and long-acting formulations of oxytetracycline reduced reactions greatly. While all the oxytetracycline treated animals produced a serological response and were immune to a 50-fold higher challenge with the immunizing stabilate, several animals in the buparvaquone groups did not show a serological response and were not immune to challenge. In the third experiment, groups of cattle were infected with 10(-1.2), 10(-1.4) and 10(-1.6) dilutions of stabilate and were treated with 2.5 mg kg-1 bwt of buparvaquone. No animals developed severe theileriosis and all seroconverted. On homologous challenge, however, two out of 14 cattle showed severe reactions. It was concluded that further work on immunization using buparvaquone treatment at 2.5 mg kg-1 bwt and 10(-1.6) dilution of the stabilate would have to be carried out before such a system could be used in the field.     

Feeding value of pastures for horses

The feeding value of fresh pasture grazed in situ is determined by animal performance or productivity and could be relatively easily established for growing and lactating horses. Despite this, there is a lack of published information on the relative feeding value of different pastures and forages grazed by horses in New Zealand and the world. In addition, for adult breeding or non-breeding and young or adult sport or performance horses, the definition of feeding value and its determination remain problematic.

Limited information suggests that the feeding value of perennial ryegrass-based pasture in New Zealand for young growing horses is high, and growth rates for Thoroughbred horses fed solely on pasture in New Zealand are similar to those reported from the Northern Hemisphere where grain-based supplements are fed in addition to pasture or other forages. Attempts to assess the ability of fresh pastures to meet the nutrient requirements of horses are hampered by problems associated with determination of feed intake by grazing horses and lack of knowledge of the digestibility and utilisation of digested nutrients, including the relative bio availability of macro- and micro-minerals in pasture. A further challenge for future research is to determine the effect of herbage allowance and grazing behaviour, including pasture species preferences, on voluntary feed intake by grazing horses.

Grazing pasture has benefits for equine health and well-being including reduced risk of some nutrition-related disorders and reduced prevalence of stereotypic behaviour. Pastured horses have greater freedom for expression of natural behaviours including social interaction and exercise. However, grazing pasture is also associated with animal health problems, particularly parasitism and diseases related to pasture-associated toxins.     

The influence of diel period on fish assemblage in the unstructured littoral of reservoirs

Abstract Diel changes in littoral fish assemblage were studied in four reservoirs in the Czech Republic (Central Europe). The sampling was performed by beach seining in an unstructured littoral zone. Perch, Perca fluviatilis L., roach, Rutilus rutilus (L.), bream, Abramis brama (L.), carp, Cyprinus carpio L., ruffe, Gymnocephalus cernua (L.), pikeperch, Sander lucioperca (L.), eel, Anguilla anguilla (L.) and A. brama × R. rutilus hybrids exhibited higher densities at night. Only bleak, Alburnus alburnus (L.) exhibited higher densities during the day. The number of species was higher in night hauls, and mean body size of roach and bleak was also higher at night. Diel changes in fish densities resulted in the change in species composition between day and night. The study has implication for the sampling design to assess littoral fish assemblages using beach seine netting and recommends night sampling for a representative assessment or sampling during both diel periods for a robust assessment.     

Rinderpest seroprevalence in wildlife in Kenya and Tanzania, 1982-1993

Eight hundred and thirty five serum samples collected from eight wild artiodactyl species in Kenya and Tanzania between 1982 and 1993 were tested for virus-neutralising (VN) antibodies to rinderpest (RP) virus. Antibodies were found in 116 of 344 buffaloes (Syncerus caffer) but not in the other species including 349 wildebeest (Connochaetes taurinus). Most of the antibody positive buffaloes were from the Maasai Mara-Serengeti ecosystem (MM-SE) and would have had opportunity for exposure to the virus during the epidemic of rinderpest in buffalo confirmed there in 1982. Buffalo born after 1985 did not have antibody indicating that virus stopped circulating in this population at or around that time. This second demonstration that RP virus disappears from the MM-SE is further evidence that these species are not permanent reservoirs of this virus. Re-infection of wildlife is transient and they remain valuable sentinels for infection in nearby domestic livestock.     

Some preliminary observations on the susceptibility and resistance of different cattle breeds to Theileria parva infection

Theileria parva-na?ve Friesian (Bos taurus), Boran (Bos indicus) and Maasai Zebu steers (B. indicus) were infected with a T. parva sporozoite stabilate dose which had previously been shown to induce an estimated 50% mortality rate in Boran cattle. All the cattle developed patent infections with no significant differences in the length of the prepatent period to development of macroschizonts (P > 0.05) between the three groups. Clinical theileriosis occurred in all eight the Friesians (100%), five out of nine Borans (55.6%) and two out of five Zebus (40%). Three of the Friesians (37.5%), and two of the Borans (22.2%) died of theileriosis. The different cattle types were equally susceptible to the infective dose used as indicated by the length of the prepatent periods, but there was a marked difference in their development of clinical theileriosis. The gradation in resistance to disease confirms the findings of earlier less critical studies and identifies these cattle breeds as suitable for investigations into the mechanisms of resistance to theileriosis.