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Naruepon Kampa DVM Ursula Wennstrom DVM Peter Lord BVSc FRCVS Robert Twardock DVM PhD Enn Maripuu BSc Per Eksell DVM PhD Sten-Olof Fredriksson BSc 《Veterinary radiology & ultrasound》2002,43(4):383-391
Determinations of different methods of measurement of uptake of 99mTc-DTPA using scintigraphy of glomerular filtration rate (GFR) were made from 29 studies on 10 healthy beagle dogs. GFR was measured by calculating the percentage dose uptake (integral method) and rate of uptake (slope method) of 99mTc-DTPA using manual kidney regions of interest (ROI) and automatic kidney and background ROIs at different time periods of the uptake phase. These results were compared using linear regression analysis to the GFR obtained from 99mTc-DTPA plasma clearance using multiple blood samples. The best correlation coefficient between percentage DTPA uptake and GFR by DTPA clearance (r = 0.84, P < 0.001) was derived from time intervals between 30s-120s with a perirenal background ROI at 1 or 2 pixels out from the kidney ROI using automatic kidney ROI at 20% threshold. With the slope method, the best correlation coefficient (r = 0.85, P < 0.001) was obtained from time intervals between 30s-peak with the background ROI at 2 pixels out from the kidney ROI using automatic ROI at 35% threshold. The offset was higher, and the correlation varied more with different ROIs and the method was unreliable at time intervals extending beyond the peak radioactivity. Manual kidney ROIs with automatic background ROIs had slightly lower correlations. With DTPA renography both integral and slope uptake method with automatic kidney and background ROIs are accurate methods to estimate the GFR, but that the integral method is much more stable to variations in ROI size and the duration of the uptake phase of the renogram. 相似文献
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Thawatchai Ngamsiri Masamichi Nakajima Srijanya Sukmanomon Naruepon Sukumasavin Wongphatom Kamonrat Uthairat Na-Nakorn Nobuhiko Taniguchi 《Fisheries Science》2007,73(4):792-799
The Mekong giant catfish Pangasianodon gigas is endemic to the Mekong River and is a critically endangered species. The genotypes of the microsatellite DNA (msDNA) and
mitochondrial DNA (mtDNA) markers (right domain of the control region) were detected to evaluate the present status of genetic
divergence of this species from the Mekong River in Thailand and Cambodia. The observed and expected heterozygosity values
of Mekong giant catfish in Thailand and Cambodia were relatively low in comparison with those of other nonendangered freshwater
fish species. These two populations from Thailand and Cambodia showed similar levels of genetic diversity, as evaluated by
the 384 nucleotides of the mtDNA control region with 13 haplotypes. The pairwise F
ST value between the two populations based on the genotype frequencies of msDNA and mtDNA markers suggested a close genetic
relationship between the populations in Thailand and Cambodia. The results of this study support the conclusion that the Mekong
giant catfish is critically endangered. Care should be taken to sustain the genetic diversity of this species, as the level
of genetic variability has already decreased in the wild population. This species is a target species for an ongoing stock
enhancement program in the Mekong River in Thailand. It is proposed to apply these markers for proper broodstock management,
such as for minimal kinship selective breeding in the hatchery. 相似文献
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Frendin JH Boström IM Kampa N Eksell P Häggström JU Nyman GC 《American journal of veterinary research》2006,67(12):1967-1973
OBJECTIVE: To investigate effects of carprofen on indices of renal function and results of serum bio-chemical analyses and effects on cardiovascular variables during medetomidine-propofol-isoflurane anesthesia in dogs. ANIMALS: 8 healthy male Beagles. PROCEDURES: A randomized crossover study was conducted with treatments including saline (0.9% NaCl) solution (0.08 mL/kg) and carprofen (4 mg/kg) administered IV. Saline solution or carprofen was administered 30 minutes before induction of anesthesia and immediately before administration of medetomidine (20 microg/kg, IM). Anesthesia was induced with propofol and maintained with inspired isoflurane in oxygen. Blood gas concentrations and ventilation were measured. Cardiovascular variables were continuously monitored via pulse contour cardiac output (CO) measurement. Renal function was assessed via glomerular filtration rate (GFR), renal blood flow (RBF), scintigraphy, serum biochemical analyses, urinalysis, and continuous CO measurements. Hematologic analysis was performed. RESULTS: Values did not differ significantly between the carprofen and saline solution groups. For both treatments, sedation and anesthesia caused changes in results of serum biochemical and hematologic analyses; a transient, significant increase in urine alkaline phosphatase activity; and blood flow diversion to the kidneys. The GFR increased significantly in both groups despite decreased CO, mean arterial pressure, and absolute RBF variables during anesthesia. CONCLUSIONS AND CLINICAL RELEVANCE: Carprofen administered IV before anesthesia did not cause detectable, significant adverse effects on renal function during medetomidine-propofol-isoflurane anesthesia in healthy Beagles. 相似文献
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Frida Westgren Charles J. Ley Naruepon Kampa Peter Lord 《Veterinary radiology & ultrasound》2014,55(6):632-637
The current standard scintigraphic method for estimating glomerular filtration rate (GFR) in dogs is the integral method, which normalizes renal GFR to body weight. The plasma volume method, that is normalizing GFR to plasma volume, has been reported to be more physiologically correct. The aim of this prospective study was to test the effect of hydration status on GFR measured by these two methods in a group of dogs with suspected renal disease. Eleven dogs were recruited. All dogs underwent standardized scintigraphic examinations before and after 15 ml/kg of fluid was administered intravenously at 5–7 ml/kg/min. Individual kidney GFR estimates (n = 22) were calculated using both methods and a consensus of two observers who were unaware of clinical findings. Individual kidney GFR increased significantly (P = 0.0008) after fluid administration using the integral method and individual kidney GFR using the plasma volume method remained constant. Percentage differences for individual kidney GFR before and after fluid administration were 31.4 ± 58.1% (change ± 95% CI) for the integral method and 0.1 ± 70% (change ± 95% CI) for the plasma volume method. Intravenously administered fluid increased individual kidney GFR from low to normal in 10 of 22 kidneys using the integral method and in 1 of 22 kidneys using the plasma volume method. Findings supported the use of the plasma volume method for scintigraphic calculation of GFR in dogs with suspected renal disease and indicated that errors of kidney status classification may more likely occur when the integral method is used. 相似文献
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