Gastric ulceration in horses: 91 cases (1987-1990).

Gastroendoscopy was performed on 111 horses (1 to 22 years old) that had signs of abdominal discomfort of variable duration and severity. At least 1 episode of colic had been observed within 48 hours of examination in 31 horses. Recurrent episodes of colic were observed in 28 horses within 2 to 10 days of examination, 31 horses within 11 to 30 days, 12 horses within 31 to 60 days, and in 9 horses at more than 60 days after the initial examination. Gastric ulceration was found in 91 of 111 horses examined. Other abnormalities involving the gastrointestinal tract or other abdominal viscera were not found on examination in 57 of 91 horses with gastric ulcers. The most frequent concurrent abnormalities found in the remaining 34 horses with gastric ulcers were impaction of the large colon (n = 6), colonic tympany (n = 6), peritonitis (n = 6), gastric impaction (n = 4), ileocecal intussusception (n = 3), small-colon impaction (n = 4), and proximal enteritis (n = 2). Thirteen horses with gastric ulceration underwent abdominal surgery, and in 5 horses, lesions were not found at surgery. Gastric ulceration was determined to be the primary cause of colic in 31 horses on the basis of the lack of other abnormalities, clinical response to treatment with histamine type-2 receptor (H2) antagonists, and confirmation of improvement or resolution of gastric ulceration via endoscopy. Gastric ulceration was the suspected cause of colic in 26 other horses on the basis of the lack of other abnormalities, severity of lesions, and clinical response to treatment with H2 antagonists.(ABSTRACT TRUNCATED AT 250 WORDS)     

Intracellular absorption difference spectrum of Limulus extra-ocular photolabile pigment

Microspectrophotometric measurements on single cell bodies located on the surface of the"lateral olfactory nerve" of Limulus polyphemus show they contain a photolabile pigment with a absorption-difference spectrum similar in shape to that of most other visual pigments and with a maximum absorption at 529 +/- 5 nanometers.     

The effect of intra-articular methylprednisolone acetate and exercise on equine carpal subchondral and cancellous bone microhardness

Dorsal carpal osteochondral injury is a major cause of lameness in horses undergoing high intensity training. Intra-articular corticosteroid treatment is used commonly to manage exercise-associated articular pain, but its use remains highly controversial in the equine athlete. This project, therefore, aimed to compare the mechanical properties of intra-articular MPA and diluent-treated middle carpal subchondral and cancellous bone in horses undergoing a short-term treadmill exercise programme. It was hypothesised that subchondral and cancellous bone mechanical properties are influenced by intra-articular administration of methylprednisolone acetate (MPA). Eight 2-year-old female horses had MPA or diluent administered into contralateral middle carpal joints at 14 day intervals, for a total of 4 treatments per horse. Horses underwent a standard treadmill exercise protocol until euthanasia (Day 70). Standard sites were located on the dorsal aspect of third, radial and intermediate carpal bones. Osteochondral samples from each test site were divided into subchondral bone and cancellous bone portions. These were dried, resin-embedded and gold-coated. Microhardness measurements were obtained at each test site. No significant effect of intra-articular treatment was detected. At each site, cancellous bone trabecular struts had an 18-19% higher microhardness value than the overlying subchondral bone. These findings indicate that intra-articular administration of MPA at this dose has no effect on subchondral or cancellous bone adaptation to short-term exercise and, therefore, on the propensity of carpal bones to injury. Further investigation into the calcified cartilage layer, effect of different corticosteroid preparations and diffusion of medication are required.     

Intellectual property. Enhanced: intellectual property landscape of the human genome

The impact of gene patents on downstream research and innovation are unknown, in part because of a lack of empirical data on the extent and nature of gene patenting. In this Policy Forum, the authors show that 20% of human gene DNA sequences are patented and that some genes are patented as many as 20 times. Unsurprisingly, genes associated with health and disease are more patented than the genome at large. The intellectual property rights for some genes can become highly fragmented between many owners, which suggests that downstream innovators may face considerable costs to gain access to gene-oriented technologies.     

Diagnostic detection methods for Mycobacterium avium subsp. paratuberculosis in white-tailed deer

This study compares the results and suitability of serological testing, microscopic examination, deoxyribonucleic acid (DNA) detection, and bacterial culture for detecting Mycobacterium avium subsp. paratuberculosis (Map) infection in asymptomatic farmed white-tailed deer (WTD) (Odocoileus virginianus). Deer were classified as infected if culture slants from their feces, lymph nodes, or ileum were positive, or if a polymerase chain reaction (PCR) assay detected Map DNA in any of its tissues. Deer identified as positive by agar gel immunodiffusion (AGID) testing or enzyme-linked immunosorbent assay (ELISA) but not by bacterial culture, Ziehl-Neelsen staining, or PCR assay were classified as suspect. Culture of tissues classified 10/16 (62.5%), histopathologic examination 1/16 (6.3%), tissue smears 4/16 (25%), culture slant (CS)-PCR on feces 12/15 (80%), CS-PCR on tissue 13/16 (81.3%), and direct PCR on uncultured tissues 5/16 (31.3%) deer as infected. The ELISA classified 2/15 (13.3%) deer as positive and therefore suspect. The AGID test was negative for all deer. Fifteen of 16 deer were positive by 1 or more tests; only 1 deer was negative on all 11 assays. The CS-PCR gave superior results on antemortem fecal testing as well as postmortem tissue testing and can be recommended for improving the detection of Map in WTD at every stage of infection.     

Polar Volatiles on Mars--Theory versus Observation: Excess solid carbon dioxide is probably present in the north residual cap

The residual frost caps of Mars are probably water-ice. They may be the source of the water vapor associated with seasonal polar hoods. A permanent reservoir of solid CO(2) is also probably present within the north residual cap and may comprise a mass of CO(2) some two to five times that of the present atmosphere of Mars. The martian atmospheric pressure is probably regulated by the temperature of the reservoir and not by the annual heat balance of exposed solid CO(2) (37). The present reservoir temperature presumably reflects a long-term average of the polar heat balance. The question of a large permanent north polar cap is reexamined in light of the Mariner 9 data. The lower general elevation of the north polar region compared to the south and the resulting occurrence in the north of a permanent CO(2) deposit are probably responsible for the differences in size and shape of the two residual caps. The details of the processes involved are less apparent, however. It might be argued that the stability of water-ice deposits depends on both insolation and altitude. The present north and south residual caps should be symmetrically located with respect to such a hypothetical stability field. However, the offset of the south cap from the geometrical pole, the non-symmetrical outline of the north cap, and the apparently uniform thickness of the thin, widespread water-ice all argue against control by simple solid-vapor equilibrium of water under present environmental conditions. We think that the present location of the water-ice may reflect, in part, the past location of the permanent CO(2) reservoir. The extreme stability of polar water-ice deposits increases the likelihood that past environmental conditions may be recorded there. Detailed information on elevations in the vicinity of the residual caps is needed before we can further elucidate the nature and history of the residual caps. This, along with measurements of polar infrared emission, should be given high priority in future missions to Mars. Two conclusions follow from the limitation of the mass of solid CO(2) on Mars at present to two to five times the mass of CO(2) in the atmosphere. If all of this CO(2) was entirely sublimated into the atmosphere as a result of hypothetical astronomical or geophysical effects, the average surface pressure would increase to 15 to 30 mbar. Although such a change would have considerable significance for eolian erosion and transportation, there seems to be little possibility that a sufficiently earthlike atmosphere could result for liquid water to become an active erosional agent, as postulated by Milton (38). The pressure broadening required for a green-house effect requires at least 10 to 20 times more pressure (39). If liquid water was ever active in modifying the martian surface, it must have been at an earlier epoch, before the present, very stable CO(2)/H(2)O system developed. There can be no intermittent earthlike episodes now. Furthermore, the present abundance of CO(2) on Mars may be an indicator of the cumulative evolution of volatiles to the surface of the planet (40). Thus, even the possibility of an earlier earth-like episode is dimmed. On Mars, the total CO(2) definitely outgassed has evidently been about 60 +/- 20 g/cm(2). On the earth, about 70 +/- 30 kg/cm(2) of CO(2) have been released to the surface (41). Hence, the total CO(2) devolved by Mars per unit area is about 0.1 percent of that evolved by the earth. Thus, the observational limits we place on solid CO(2) presently located under the north residual cap also may constitute considerable constraints on the total differentiation and devolatilization of the planet. If they are valid, it would seem unlikely that Mars has devolatilized at all like the earth, or ever experienced an earthlike environment on its surface.