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An intra-abdominal testicular tumour was diagnosed in a 10-year-old, male, cryptorchid golden retriever. Upon surgical removal, torsion of the testis was found. The histopathological diagnosis was seminoma. The ultrasound examination in this case revealed a parenchymal mass lesion. An ultrasound examination is a useful supplemental diagnostic tool for differentiation of abdominal mass lesions. 相似文献
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T Miyabayashi D S Biller P A Manley K J Matushek 《Journal of the American Veterinary Medical Association》1991,199(5):598-600
A flexed dorsoplantar radiographic view of the talocrural joint was a useful additional view to diagnose abnormalities of the lateral trochlear ridge of the talus of 2 dogs. This view outlined the subchondral bone of both trochlear ridges of the talus and the apposing cochlea tibiae of the distal portion of the tibia. The tarsus was flexed at the level of the talocrural joint, and an x-ray beam was centered on the joint. With this additional view, fractures of the lateral trochlear ridge were readily diagnosed. This view would help to demonstrate osteochondral lesions of the lateral trochlear ridge. 相似文献
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Joe P. Morgan DVM Vet med dr Takayoshi Miyabayashi BVS MS 《Veterinary radiology & ultrasound》1988,29(2):72-77
A group of 160 Beagles were studied radiographically to determine the pattern of degenerative changes within the vertebral column, especially involving the intervertebral discs. The normal radiographic appearance of both disc and surrounding vertebrae is described. Disc space narrowing and calcification of discal tissues provide radiographic patterns that assist in diagnosis and prognosis. Because of the older age of the dogs, severe degeneration of the endplates with marked instability between vertebral segments was seen. 相似文献
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Takayoshi Miyabayashi BVS MS PhD Mark Smith BS RT MS † Yoshihiro Tsuruno BVSc 《Veterinary radiology & ultrasound》2000,41(4):308-312
Various magnetic resonance (MR) imaging techniques have been used to assess lumbar spinal abnormalities in people. Four, young adult, clinically normal dogs were used to compare images of the spinal cord acquired using conventional spin-echo and rapid acquisition relaxation-enhanced (RARE), commonly called fast spin-echo (FSE), magnetic resonance imaging techniques. Lateral myelograms were made as an anatomic control. The T2-weighted FSE technique was characterized by better image quality than the T2-weighted conventional spin-echo technique. The short acquisition time with the FSE technique allowed increases in the matrix size and number of excitations, thus improving resolution and signal-to-noise ratio. In canine lumbar spinal MR imaging, use of a FSE technique is recommended to reduce the overall time for imaging and to improve image quality. 相似文献
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Kamemori N Takeuchi T Sugiyama A Miyabayashi M Kitagawa H Shimizu H Ando K Harada E 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(3):313-315
The transportation of intravenously administered bovine lactoferrin (bLF) into the cerebrospinal fluid (CSF) was immunohistochemically investigated in adult rats. Administered bLF was detected in the vesicular membranes of endothelial cells in cerebral blood vessels 10 min after the infusion. Numerous immunoreactive small vesicles were also detected at the ependymal cells in the choroid plexus. Moreover, the bLF concentration in the CSF was significantly increased at 1-2 hr after the intravenous infusion of bLF (10 or 30 mg/kg). These findings clearly demonstrate that LF is possibly transported into the brain matter even in adult animals. 相似文献
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Spondylosis deformans is a generalised disease of ageing resulting in formation of bony osteophytes around degenerating intervertebral discs throughout the vertebral column. It is noninflammatory and needs to be differentiated from spondylitis, which is either an infectious or noninfectious inflammatory process. Lateral radiographs of 310 female beagle dogs from a closed colony showed development of spondylosis deformans that was age-dependent and located principally at discs numbers 5, 15, and 20 to 23. It was noteworthy that disc 26 was minimally involved whereas this location has been previously reported to have frequent involvement in larger breeds of dogs. None of the dogs studied had clinical signs causing their removal from the colony, further supporting the concept that spondylosis deformans as a bony response to disc degeneration is not a cause of severe pain or lameness. 相似文献
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Proliferation of canine intervertebral disk chondrocytes in three-dimensional alginate microsphere culture 总被引:2,自引:0,他引:2
Masuda K Miyabayashi T Meachum SH Eurell TE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(1):79-82
Proliferation of chondrocytes from nucleus pulposus (NP) and anulus fibrosus (AF) was confirmed in three-dimensional culture using alginate microspheres. Cells isolated from NP and AF were incorporated in microspheres and cultured for 14 days. Round mononuclear cells of 20-25 microm in diameter proliferated and formed aggregates. At day 14, alcian blue positive matrix surrounded the proliferating cells. The cells had cytoplasmic vacuoles stained positively by toluidine blue. On electron microscopy, the cells contained proteoglycan vacuoles and lipid droplets in the cytoplasm and synthesized collagen fibrils and electron dense granules surrounding the cell. These features of the cells were characteristic for chondrocytes. This culture system should be useful to further investigate metabolic activities of intervertebral disk chondrocytes. 相似文献
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Maeda S Miyabayashi T Yamamoto JK Roberts GD Lepine AJ Clemmons RM 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(12):1373-1376
To investigate temporal dynamic changes in the synthesis of chondroitin 6-sulfate (CS6) and chondroitin 4-sulfate (CS4) in vitro, normal articular cartilage of femoral heads was harvested from three dogs. Chondrocytes were isolated and cultured in alginate microspheres for 21 days. On days 7, 14 and 21, DNA content was quantified by fluorometric assay using Hoechst 33258. On days 14 and 21, proteoglycans were extracted, and the amounts of CS6 and CS4 were quantified after chondroitinase ABC digestion using capillary electrophoresis. The DNA content and amounts of CS6 and CS4 increased during the culture period. The amounts of CS6 and CS4 divided by DNA content revealed that the synthesis of CS6 was more up-regulated than CS4. 相似文献