睾丸支持细胞的功能、分离纯化及鉴定研究进展

支持细胞对维持精子形成过程中的微环境起决定作用,它可以通过分泌功能、细胞间连接形成的血睾屏障功能以及吞噬功能等来促进精子的形成过程,其发育异常会导致不同程度的雄性生殖缺陷。基于支持细胞在雄性动物生殖过程中的作用,体外培养高纯度支持细胞可成为研究睾丸两大核心功能-精子发生和性激素分泌功能相关调节机制重要的细胞模型。此外,体外培养睾丸支持细胞也可作为生殖毒理学等新兴热点领域的细胞模型,为评估和研究环境因素对雄性生殖的影响提供便利。因此,作者系统地归纳、总结了目前关于动物支持细胞生物功能的研究及常用的体外分离纯化、培养及鉴定方法,以期为利用动物支持细胞开展雄性生殖领域的研究提供参考。     

Resettling,disconnecting or displacing? Attending to local sociality,culture and history in disaster settings

In many disaster settings, top‐down responses emphasise ‘expert‐led’ solutions that often involve relocating disaster‐affected communities. While the intention might be to move people from harm's way and facilitate recovery, failure to attend to local pre‐disaster circumstances as well as the interplay between power, resilience and vulnerability within and around affected communities often sees resettlement reconfigure as displacement or disconnection. This oversight may even usher in a new phase of dispossession and disadvantage for marginalised groups (particularly in colonial settings). This paper explores experiences in Australia, Japan and Taiwan to reflect on what issues of local sociality, local culture and local resilience need to be attended to in framing ‘better’ disaster responses.     

从不同源杂交种选育玉米二环系的配合力研究

在玉米育种中以GCA,SCA为指标选择玉米二环系的源杂交种作为基础材料来选系,从而选择优良的玉米自交系和杂交组合,本研究结果表明,这种选育玉米自交系的方法是可行和有效的.     

除草剂对不同燕麦品种杂草防治效果及其产量的影响

为了筛选燕麦田高效安全的除草剂,在山西北部地区开展了田间试验,选取田普除草剂(主要成分为二甲戊灵)为试验药剂,8种不同生态型的燕麦品种为供试品种,检验该除草剂对不同品种产量和安全性的影响。研究结果表明:田普除草剂对藜科、蓼科、禾本科、苋科杂草效果较明显,尤其是对藜科杂草的防治效果最好,最高的达到了100%,而对马齿苋科、菊科、旋花科杂草效果不好;使用田普除草剂处理对燕麦品种的穗长、轮层数、小穗数等性状有一定的抑制作用,对籽实千粒重的有促进作用;本研究中喷施除草剂的处理的平均籽实产量比不喷施除草剂的处理和人工除草处理分别增产70.5%和4.3%。此外,该除草剂未对后茬作物(油菜)产生明显药害作用,可对燕麦-油菜倒茬安全使用。     

Evaluation of the perioperative stress response in dogs administered medetomidine or acepromazine as part of the preanesthetic medication

OBJECTIVE: To compare the perioperative stress response in dogs administered medetomidine or acepromazine as part of the preanesthetic medication. ANIMALS: 42 client-owned dogs that underwent elective ovariohysterectomy. PROCEDURE: Each dog was randomly allocated to receive medetomidine and butorphanol tartrate (20 microgram/kg and 0.2 mg/kg, respectively, IM) or acepromazine maleate and butorphanol (0.05 and 0.2 mg/kg, respectively, IM) for preanesthetic medication. Approximately 80 minutes later, anesthesia was induced by administration of propofol and maintained by use of isoflurane in oxygen. Each dog was also given carprofen before surgery and buprenorphine after surgery. Plasma concentrations of epinephrine, norepinephrine, cortisol, and beta-endorphin were measured at various stages during the perioperative period. In addition, cardiovascular and clinical variables were monitored. RESULTS: Concentrations of epinephrine, norepinephrine, and cortisol were significantly lower for dogs administered medetomidine. Concentrations of beta-endorphin did not differ between the 2 groups. Heart rate was significantly lower and mean arterial blood pressure significantly higher in dogs administered medetomidine, compared with values for dogs administered acepromazine. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicate that for preanesthetic medications, medetomidine may offer some advantages over acepromazine with respect to the ability to decrease perioperative concentrations of stress-related hormones. In particular, the ability to provide stable plasma catecholamine concentrations may help to attenuate perioperative activation of the sympathetic nervous system.     

Attitudes of Finnish dog-owners about programs to control canine genetic diseases

A mailed questionnaire survey was done to study dog-owners' knowledge of canine inherited diseases and the present screening and control programs in Finland, as well as to study the importance of health-related matters and well-being to dog-owners. The purpose was also to study the owners' role in programs and the influence of health and well-being in their decision-making process and choices when purchasing a puppy. This study showed that dog-owners highly valued health-related matters and well-being. The present programs were considered to have a positive effect on the prevention of canine inherited diseases. Dog-owners estimated that their own knowledge could be better but they believed in veterinarians' and dog-breeders' ability to inform them. Inherited skeletal and ocular diseases were thought to be important to canine well-being; the importance of other diseases or behaviour problems was assessed to be lower. In choosing a suitable breed, behaviour and temperament were the most-important factors but health-related matters were essential in actually choosing a puppy's breeder and the litter. Well-being and health were also considered very important to the image of the dog hobby.     

The combined effects of enhanced UV-B radiation and selenium on growth, chlorophyll fluorescence and ultrastructure in strawberry (Fragaria × ananassa) and barley (Hordeum vulgare) treated in the field

The possible ameliorative effects of selenium (Se) addition to soil on the detrimental effects of enhanced UV-B radiation were tested on strawberry and barley during 4 months of field experiment in Kuopio, Central Finland. Control plants were exposed to ambient levels of UV radiation, using arrays of unenergized lamps. A control for UV-A radiation was also included in the experiment. Added Se, applied as H₂SeO₄, at the level of 0.1 mg kg⁻¹ soil (low dosage) and 1 mg kg⁻¹ soil (high dosage) increased Se concentrations in plants more than 10 and 100 times, respectively. After 4 months of exposure, strawberry and barley plants were harvested for biomass analysis. Chlorophyll fluorescence was measured using the Hansatech FMS2 fluorescence monitoring system. Leaf anatomy and ultrastructure were observed by light and transmission electron microscope. Several effects of UV and Se as well as their interaction were found, mostly for strawberry, but not for barley, indicating species-specific responses. Our results provided evidence that the high Se concentration in soil had no ameliorative effect but increased the sensitivity of strawberry to enhanced UV-B radiation in the field. Under ambient radiation, Se did not alter leaf growth of strawberry, whereas under UV-B radiation, the high Se addition significantly decreased leaf growth. Strawberry runner biomass was affected by the interaction of Se and UV. Under ambient radiation Se did not change dry weight of runners, but in combination with UV-A or UV-B radiation the high Se dosage decreased dry weight of runners by about 30%. Although the high Se concentration positively influenced on quantum efficiency of photosystem II (PSII) in strawberry leaves, it reduced runner biomass, leaf number and ratio of starch to chloroplast area. This suggests that the harmful effects of the high Se dosage on photosynthetic processes occurred as a result of changes in activity or/and biosynthesis of enzymes, rather than alteration of PSII. At the low concentration, Se effects were slight and variable.Although barley leaves accumulated higher Se concentrations than strawberry, there were no apparent changes in their growth, biomass or chlorophyll fluorescence due to Se effect either alone or in combination with UV-B. However, at the ultrastructural level, an enlargement in the peroxisome area was found due to combination of UV radiation with Se, suggesting the activation of antioxidative enzymes, possibly catalase. Decrease in mitochondrial density in barley cells in response to Se might be attributed to alteration of mitochondrial division. Increase in the proportion of cells with cytoplasmic lipid bodies due to combined effect of UV-B and Se indicated the alteration of lipid metabolism and the acceleration of cell senescence in barley. Main UV-B effects were found, mostly at the tissue and ultrastructural level in strawberry, but not in barley, indicating species-specific susceptibility to enhanced UV-B radiation. UV-B-treated strawberry plants developed marginally thinner leaves with reduced ratio of starch to chloroplast area in their cells, suggesting negative influence of UV-B on photosynthetic processes.