红脂大小蠹高效引诱剂林间引诱试验结果初报

红脂大小蠹是原产北美近年传入我国的一种危险性森林害虫 ,生活隐蔽 ,防治难度很大。为筛选出对我国油松林中红脂大小蠹种群有高效引诱作用的药剂 ,为今后控制红脂大小蠹的危害提供经济、实用、高效的监测方法和防治技术提供技术和物质依据 ,作者于 2 0 0 2年 5～ 7月在石家庄井陉和邢台内丘两地 ,先后进行了连续 2次的引诱试验。试验以加拿大生产的商品和松节油分别作为对照 ,共对 2 7种不同药剂的林间引诱效果进行了测定。试验结果表明 ,2B -7号引诱剂引诱效果显著好于加拿大对照 (a=0 .0 1 4) ,每个诱捕器平均诱虫数量比加拿大引诱剂多 96.3 9%。初步测算其成本比购买加拿大产品低 3 0 %～ 5 0 %以上。     

Neurobiological substrates of dread

Given the choice of waiting for an adverse outcome or getting it over with quickly, many people choose the latter. Theoretical models of decision-making have assumed that this occurs because there is a cost to waiting-i.e., dread. Using functional magnetic resonance imaging, we measured the neural responses to waiting for a cutaneous electric shock. Some individuals dreaded the outcome so much that, when given a choice, they preferred to receive more voltage rather than wait. Even when no decision was required, these extreme dreaders were distinguishable from those who dreaded mildly by the rate of increase of neural activity in the posterior elements of the cortical pain matrix. This suggests that dread derives, in part, from the attention devoted to the expected physical response and not simply from fear or anxiety. Although these differences were observed during a passive waiting procedure, they correlated with individual behavior in a subsequent choice paradigm, providing evidence for a neurobiological link between the experienced disutility of dread and subsequent decisions about unpleasant outcomes.     

Morphology and distribution of granulomatous inflammation in freshwater ornamental fish infected with mycobacteria

Mycobacteriosis in fish is a chronic progressive ubiquitous disease caused by Mycobacterium marinum, M. gordonae and M. fortuitum in most cases. The aim of this study was to describe the morphology and distribution of lesions in 322 freshwater ornamental fish across 36 species. Granulomatous inflammation was diagnosed by gross examination and histopathology testing in 188 fish (58.4%); acid‐fast rods (AFR) were determined in only 96 (51.1%) fish from 19 species after Ziehl–Neelsen staining. The most often affected organs with AFR were the kidney (81.2%), digestive tract (54.1%), liver (48.2%), spleen (45.9%) and skin (21.2%); sporadically, AFR were found in the branchiae (9.4%) and gonads (4.7%). In 14 randomly selected fish originating from four different fish tanks, the distribution of mycobacterial infection was studied by culture examination of the skin, gills, muscle tissue, digestive tract, liver, spleen and kidney. In 12 fish, the species M. marinum, M. gordonae, M. fortuitum, M. triviale, and M. avium subsp. hominissuis (serotypes 6 and 8 and genotype IS901? and IS1245+) were detected; mixed infection caused by different mycobacterial species was documented in five of them.     

A rapid real-time PCR assay for detecting columnar growth in apples (Malus × domestica)

Columnar growth in apples can have considerable positive consequences for orchard maintenance, but the lack of columnar cultivars producing fruits with desired market quality prevents their broader utilization in orchards. New crossings focused on their breeding are therefore conducted throughout the world every year. Unfortunately, the reliable discrimination between columnar growth and normal phenotype is difficult until seedlings are 2–3 years of age. However, molecular marker–assisted selection (MAS) can be used in seedlings several weeks old providing immense help in breeding programmes focused on columnar apple production. Here, we describe a rapid and reliable method for the detection of both wild-type (WT) and columnar (Co) alleles originating from ‘McIntosh Wijcik’ in a single reaction using a real-time PCR allelic discrimination approach. Our assay was tested in 130 genotypes (80 normal and 50 columnar habit) from various crossings and their parents, and in all cases, genotype corresponded to phenotype. The assay is thus suitable for laboratories interested in high-throughput MAS for columnar tree habit breeding.     

Agmatine protection against chlorpromazine-induced forebrain cortex injury in rats

This study was conducted to investigate whether agmatine (AGM) provides protection against oxidative stress induced by treatment with chlorpromazine (CPZ) in Wistar rats. In addition, the role of reactive oxygen species and efficiency of antioxidant protection in the brain homogenates of forebrain cortexes prepared 48 h after treatment were investigated. Chlorpromazine was applied intraperitoneally (i.p.) in single dose of 38.7 mg/kg body weight (BW) The second group was treated with both CPZ and AGM (75 mg/kg BW). The control group was treated with 0.9% saline solution in the same manner. All tested compounds were administered i.p. in a single dose. Rats were sacrificed by decapitation 48 h after treatment Treatment with AGM significantly attenuated the oxidative stress parameters and restored antioxidant capacity in the forebrain cortex. The data indicated that i.p. administered AGM exerted antioxidant action in CPZ-treated animals. Moreover, reactive astrocytes and microglia may contribute to secondary nerve-cell damage and participate in the balance of destructive vs. protective actions involved in the pathogenesis after poisoning.     

Influence of Cow Age on Grazing Distribution in a Mixed-Conifer Forest

Optimal distribution of cattle on forested rangelands has long been a subject of concern specifically related to uniform and sustainable use of forage resources. Our objective was to determine if cow age influenced distribution and resource use on forested rangelands. This study was conducted from 1991 to 2001 at the US Department of Agriculture Starkey Experimental Forest and Range, northeastern Oregon, a mixed-conifer forested rangeland. We used 43 039 locations of cattle taken from 1 h prior to sunrise until 4 h after sunrise and 4 h prior to sundown until 1 h after sundown from 15 July to 30 August to evaluate cattle distribution patterns during peak foraging time. Cattle were grouped into four age classes: 2- and 3-yr-old cattle, 4- and 5-yr-old cattle, 6- and 7-yr-old cattle, and cattle ≥ 8 yr old. All age classes preferred areas with gentler slopes (P &spilt; 0.05), westerly aspects (P &spilt; 0.05), farther from water (P &spilt; 0.05), and with greater forage production (P &spilt; 0.05) than pasture averages. Cattle older than 3 yr of age selected areas with less canopy closure (P &spilt; 0.05) than the mean value for the pasture. Young cows (&spilt; 4 yr old) selected lower elevations and steeper slopes than the oldest cows (P &spilt; 0.05). In summary, cow age and correspondingly its experience directly influences distribution patterns and forage resource use of cattle at the Starkey Experimental Forest and Range.     

Biodiversity,exotic plant species,and herbivory: The good,the bad,and the ungulate

Invasion of natural ecosystems by exotic plant species is a major threat to biodiversity. Disturbance to native plant communities, whether natural or management induced, is a primary factor contributing to successful invasion by exotic plant species. Herbivory by both wild and domestic ungulates exerts considerable impact on structure and composition of native plant communities. Intensive herbivory by ungulates can enhance exotic plant invasion, establishment, and spread for three reasons: (1) many exotic plants are adapted to ground disturbances such as those caused by ungulate feeding, trampling, and movements; (2) many exotic plants are adapted for easy transport from one area to another by ungulates via endozoochory and epizoochory; (3) many exotic plants are not palatable or are of low palatability to ungulates, and consequently, their survival is favored as ungulates reduce or eliminate palatable, native plants. Ungulate herbivory is a chronic, landscape-scale disturbance capable of influencing plant communities as much as episodic events such as fire. Consequently, ungulate herbivory has the potential to facilitate the invasion and establishment of exotic plants in the interior Pacific Northwest where ungulates occupy nearly every ecosystem. Moreover, ungulate herbivory has intensified in many ecosystems, owing to the addition of domestic ungulates with that of existing, wild ungulates, coupled with the reduction or elimination of migratory movements and predators that previously regulated wild ungulate populations and influenced their distributions. Despite the observational evidence for ungulate herbivory as a strong facilitator of exotic plant invasion and establishment, current knowledge of cause–effect relations is severely limited by a lack of manipulative experiments. Most studies have been observational, unreplicated, and lack the experimental controls needed to eliminate or account for confounding sources of variation. Heightened attention to conservation of biodiversity will increase the importance of managing ungulates in balance with the plant communities that support them.     

Zur Weiterentwicklung eines halbsynthetischen Nährmediums fürIps typographus L.

The semiartificial diet for the bark beetleIps typographus L. ofSimsek andFührer (1993) andSimsek (1995) was further developed in its handling and composition of the vitamin solution. The biological availability of the vitamins of the vitamin b-group was investigated. The diet is useful as rearing medium for larvae, pupae and adult beetles. But it is not possible by now to grow reproducibly a whole generation cycle on this diet.