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1.
Seiji TSUGE Ayako FURUTANI Rie FUKUNAKA Yasuyuki KUBO Osamu HORINO 《Journal of General Plant Pathology》2001,67(1):51-57
Xanthomonas oryzae pv. oryzae (X. o. pv. oryzae) T7174 is virulent on rice cultivar IR24 and avirulent on IR-BB2. From recent reports, some virulence and avirulence factors
of plant pathogenic bacteria are transferred to plant cells through the hrp-dependent type III secretion system. In this study, we investigated the involvement of hrp genes in the compatible and the incompatible interactions between rice and X. o. pv. oryzae after co-inoculation with hrpXo mutants derived from T7174 and virulent strains. Growth of the mutants, named 74ΔHrpXo and 76ΔHrpXo, was repressed in IR24 when the mutants were applied alone. However, growth of the mutants was complemented by co-inoculation
with virulent strains. Growth of bioluminescent hrpXo mutant 76ΔHrpXo in IR24 and its growth in IR-BB2 after co-inoculation with T7133, which is virulent on both cultivars, was equally complemented,
as detected by bioluminescence from the mutant. On the other hand, only partial complementation of growth of T7174L76, which
is a bioluminescent and pathogenic derivative of T7174, by T7133 was observed in IR-BB2. Thus, growth of the hrpXo mutant of X. o. pv. oryzae was complemented by virulent strains in both susceptible and resistant rice leaves with the parental strain.
Received 21 July 2000/ Accepted in revised form 26 October 2000 相似文献
2.
Shotaro KUBO Morihiro TATENO Yasuaki ICHIKAWA Yasuyuki ENDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1275-1279
Tick-borne diseases are often encountered in canine clinical practice. In the present
study, a molecular epidemiological survey of dogs in Japan was conducted to understand the
prevalence and geographical distribution of Babesia spp.,
Hepatozoon spp., Ehrlichia spp. and
Anaplasma spp. Pathogen-derived DNA in blood samples obtained from 722
dogs with a history of exposure to ticks and/or fleas was examined by PCR. The prevalence
of Babesia gibsoni, Babesia odocoilei-like species,
Hepatozoon canis and Ehrlichia
spp./Anaplasma spp. was 2.4% (16/722), 0.1% (1/722), 2.5% (18/722) and
1.5% (11/722), respectively. While B. gibsoni and
Ehrlichia spp./Anaplasma spp. were detected in the
western part of Japan, H. canis was detected in Tohoku area in addition
to western and central parts of Japan. 相似文献
3.
Noritaka ADACHI Daisuke YAMAGUCHI Akiyuki WATANABE Narumi MIURA Seiji SUNAGA Hitoshi OISHI Michiko HASHIMOTO Takatsugu OISHI Masaki IWAMOTO Hirofumi HANADA Masanori KUBO Akira ONISHI 《The Journal of reproduction and development》2014,60(2):100-105
The objective of this study was to examine the health and meat production of cloned sows
and their progenies in order to demonstrate the application of somatic cell cloning to the
pig industry. This study compared the growth, reproductive performance, carcass
characteristics and meat quality of Landrace cloned sows, F1 progenies and F2 progenies.
We measured their body weight, growth rate and feed conversion and performed a
pathological analysis of their anatomy to detect abnormalities. Three of the five cloned
pigs were used for a growth test. Cloned pigs grew normally and had characteristics
similar to those of the control purebred Landrace pigs. Two cloned gilts were bred with a
Landrace boar and used for a progeny test. F1 progenies had characteristics similar to
those of the controls. Two of the F1 progeny gilts were bred with a Duroc or Large White
boar and used for the progeny test. F2 progenies grew normally. There were no biological
differences in growth, carcass characteristics and amino acid composition among cloned
sows, F1 progenies, F2 progenies and conventional pigs. The cloned sows and F1 progenies
showed normal reproductive performance. No specific abnormalities were observed by
pathological analysis, with the exception of periarteritis in the F1 progenies. All pigs
had a normal karyotype. These results demonstrate that cloned female pigs and their
progenies have similar growth, reproductive performance and carcass quality
characteristics and that somatic cell cloning could be a useful technique for conserving
superior pig breeds in conventional meat production. 相似文献
4.
Masamitsu TOMIYAMA Shouhei KUBO Tsutomu TAKAGI Keiichi SUZUKI 《Animal Science Journal》2011,82(5):621-626
Genetic improvement of the reproductive performance of pigs is important for pig breeding despite their low heritabilities. The objectives of this study were to investigate the effectiveness of selection concerning reproductive traits and to determine the optimal number of parity records required for accurate estimation of breeding values (BVs) in the open population of a commercial pig breeding company. The study used records of 2220 purebred Large White pigs (9845 litters) farrowed between 1998 and 2009 in the two herds of the Pacific Ocean Breeding Co. Ltd. The traits studied included farrowing interval (FI), total number of piglets at birth (TNB), average weaning weight per litter (AWW), and raising rate (RR). A statistical model was applied to the 4‐trait repeatability animal model. The heritabilities of FI, TNB, AWW and RR were low. The genetic trends in TNB (h2 = 0.09) showed approximately 1.0 increase in 6 years from 2003 to 2008. The predicted error variances indicated that up to fourth parity records are necessary for accurate genetic evaluation. The present study results indicated that even reproductive traits with low heritability can be improved. 相似文献
5.
Elisabet TANGKONDA Meiko KUBO Satoshi SEKIGUCHI Taisuke SHINKI Satomi SASAKI Kentaro YAMADA Takako TANIGUCHI Torrung VETCHAPITAK Naoaki MISAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1306
Workers in poultry abattoirs may be frequently exposed to Campylobacter jejuni, which is a leading cause of bacterial food poisoning in Japan. The present study was conducted to measure the titers of IgG and IgA antibodies against C. jejuni among 104 female workers in a chicken processing plant in Miyazaki prefecture, Japan. Information regarding habitual ingestion of raw chicken meat and potential occupational risk factors was collected using a questionnaire. Acid extracts of four C. jejuni strains representing the genotypes most dominant in Miyazaki were used as antigens. The levels of both immunoglobulins measured by ELISA were not correlated with ingestion of edible raw chicken meat, the amount consumed in one sitting, or its frequency. Although age was correlated with antibody levels, the length of employment was not. Furthermore, the IgG and IgA levels in workers at the evisceration step were significantly higher than those at other locations in the plant. To identify the bacterial proteins recognized by the workers’ IgG and IgA antibodies, Western blotting followed by LC/MS was conducted. Flagellin was identified as the common protein recognized in the sera of workers for whom ELISA demonstrated both the highest and lowest antibody levels. We concluded that the titers of IgG and IgA against C. jejuni in workers at the processing plant had been increased by occupational exposure to Campylobacter, regardless of raw chicken meat ingestion. 相似文献
6.
Seiji TSUGE Mami NISHIDA Ayako FURUTANI Yasuyuki KUBO Osamu HORINO 《Journal of General Plant Pathology》2001,67(2):144-147
We isolated Xanthomonas oryzae pv. oryzae mutants deficient in the phosphoenolpyruvate : carbohydrate phos-photransferase system, a major glucose transport system in
bacteria, using the glucose analogue 3-deoxy-3-fluoro-d-glucose (3FG). Glucose uptake by the mutants was decreased to 15–35% of the parental strain, and growth greatly decreased
in synthetic media containing glucose as a sole sugar source. Growth of the mutants in rice leaves was, however, similar to
the wild type. These findings suggest that glucose is not necessarily a major carbohydrate source for X. o. pv. oryzae in rice leaves.
Received 11 August 2000/ Accepted in revised form 15 December 2000 相似文献
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9.
Shin-Ho?Park Soon-Yeong?Cho Meiko?Kimura Hisanori?Nozawa Nobuo?SekiEmail author 《Fisheries Science》2005,71(4):896-903
ABSTRACT: The addition of microbial transglutaminase (MTGase) to salted squid muscle paste greatly strengthened the elasticity of the thermal gel, which was produced by the preferential cross-linking of myosin heavy chains through a two-step heating process that consisted of setting at 40°C and subsequent heating to 80°C or 90°C. Starch increased the breaking strength of thermal squid gels, but decreased the deformation. Thus, the starch-added gels became harder and less elastic. Although, when both MTGase and starch were added to squid muscle paste, the changes in viscoelastisity and myosin cross-linking reaction were similar to those with MTGase alone during setting, the storage and loss moduli of the paste sharply increased with an increase in starch content above 70°C. Subsequently, the thermal gel texture became more brittle or breakable than gels prepared by setting with MTGase only. 相似文献
10.
Monomeric silica solutions were mixed with solutions containing AlCl3, or Al(OH)2-55Cl0-45 to give a SiO2/Al2O3, ratio varying from 8 to 8.0 and were kept at pH 6–0, 7.0, or 8.0 over a period of about I year. All precipitates which formed from solutions with SiO2/Al2O3 ratios of 1–0 or higher were X-ray amorphous. The SiO2/Al2O2, ratio of the precipitate varied in the range from 1.0 to 3.0. It increased with the Si concentration and the SiO2/Al2O3 ratio of the parent solution and with reaction time. The pH did not affect the SiO2/Al2O3 ratio of the precipitate but did affect its dispersion and flocculation. Al3+ and Al(OH)2.55+0.45 reacted differently with silica and yielded different reaction products. These differences were interpreted in terms of the stability of hydroxy-Al polymer units in the reaction. The amount of NaOH per Al atom which was required to reach and maintain the same pH, increased with the increasing SiO2/A12O ratio of the precipitate, but the value was generally lower than that predicted from ‘one in four’ substitution of Si by Al in four-fold co-ordination. A ‘neutralization’ of the positive charge on the hydroxy-Al cations by silica was pointed out also to be a cause of the extra acidity. Implications of these observations on synthetic amorphous aluminosilicates to the genesis, structure, and properties of their natural counterparts were discussed. 相似文献