Serotypes of avian infectious bronchitis virus isolates from field cases in Japan

Eight etiologic agents isolated from field cases in Japan were identified as isolates of infectious bronchitis virus by agar-gel diffusion, buoyant-density determination on sucrose-density centrifugation, and morphological study by electron microscope of the purified viruses. In studies of the antigenic relationships of the eight isolates and six known infectious bronchitis viruses, antigenic diversity of these viruses was recognized from the degrees of relatedness using a plaque reduction in the "constant-virus decreasing-serum" method.     

Modification of three avian viruses passaged in Chinese hamster lung cells (Don) in pathogenicity to chicken embryo

The Beaudette 42 strain of avian infectious bronchitis virus, Sato strain of Newcastle disease virus, and Uchida strain of avian reovirus were passaged in Chinese hamster lung cells (Don), and some properties were examined. The Don-passaged strains showed a difference in replication in Don and chicken embryo kidney cells in one-step growth curve examinations and a partial modification in pathogenicity to chicken embryos; nevertheless, neutralization tests revealed no serological alteration.     

Seroprevalence of Bartonella henselae in Dogs and Cats in Sassari

Veterinary Research Communications - Pinna Parpaglia, M.L., Masu, G., Masala, G., Porcu, R., Zobba, R., Pintori, G. and Cocco, R., 2007. Veterinary Research Communications, 31(Suppl. 1), 317–320     

Establishment of shell growth analysis technique of juvenile Manila clam <Emphasis Type="Italic">Ruditapes philippinarum</Emphasis>: semidiurnal shell increment formation

ABSTRACT:   The conventional acetate peel method was modified to analyze the shell growth pattern of juvenile Manila clam Ruditapes philippinarum as small as 2 mm in shell length (SL). In the outer shell layer along the axis of maximum growth, two types of growth increments were observed: distinct increments and indistinct increments, which, respectively, do and do not continue to the middle shell layer. The distinct increments were found to be formed every two days in intertidal and shallow subtidal zones by field enclosure experiments of juveniles with datum points marked with alizarin complexone. Growth patterns of juveniles (12 mm SL) collected from the Seaside Park of Yokohama in Tokyo Bay were analyzed to confirm the modified method. Mean daily shell growth rate from April to July 2005 ranged 120–142 μm/day, which was reasonable as compared with previous studies. It was impossible to backcalculate the growth to the settlement size (i.e. 0.2 mm SL) because of erosion of the outer shell surface, and the smallest backcalculated minimum shell length was 0.8 mm. Fluctuations in daily growth rate were high, ranging 29–315 μm/day, and did not show a clear two-weekly rhythm.