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1.
2.
To determine the factors affecting agonistic interactions after regrouping, 24 pigs were allocated to six pens that each had a box. Half of the pigs were experienced in using the box. One castrated male and one female pig were transferred from each pen to another pen at random. The number of pigs attacked was particularly large for the pigs that had no experience in using the box and were transferred on the first day ( P < 0.05). The duration of access to the box was markedly longer for the pigs that had experience in using the box and were not transferred on the first day ( P < 0.05). The number and duration of attacks were significantly larger and longer toward unfamiliar individuals than toward familiar ones (both P < 0.01). The number of attacks toward the same sex was significantly larger than toward the opposite sex ( P < 0.01). A negative correlation was found between the number of agonistic interactions on the first day and the range of body weights in the pen mates ( r = −0.78, P = 0.07). In conclusion, regrouping with unfamiliar individuals should be avoided, but when unavoidable, the following methods are recommended to reduce agonistic interactions: (1) ensure the intruders are experienced in using a box, (2) move experienced intruders in with residents that have no experience in using a box, (3) mix different sexes, and (4) have wide variations in body weights in a pen. 相似文献
3.
Data from six primiparous and nine multiparous Holstein cows were used to clarify the difference of Ca and P mobilization between primiparous and multiparous cows during early lactation. The dry matter intake (DMI) of primiparous cows was lower (P < 0.01) than those of multiparous cows. Milk yield was lower in primiparous cows at 7, 14, 21 (P < 0.01), and 28 days (P < 0.05) after parturition. There was no significant difference in milk Ca and P concentrations between primiparous and multiparous cows. There were no significant differences in plasma Ca and P concentrations between primiparous and multiparous cows. The plasma P level at 7 days postpartum in primiparous cows was lower (P < 0.05) than 28 days postpartum. The concentration of plasma osteocalcin (OC) measured as bone formation marker of primiparous cows was significantly higher than multiparous cows (P < 0.01) at 21 and 28 days postpartum. The urinary deoxypyridinoline (DPD) as bone resorption marker of primiparous cows tended to be higher (P < 0.10) than multiparous cows at 21 days after parturition and decreased to the same level as that of multiparae toward the peak lactation. These results show that Ca and P mobilization of primiparous cows are more active than multiparous cows. 相似文献
4.
Carotenoids are not only important to the plants themselves but also are beneficial to human health. Since citrus fruit is a good source of carotenoids for the human diet, it is important to study carotenoid profiles and the accumulation mechanism in citrus fruit. Thus, in the present paper, we describe the diversity in the carotenoid profiles of fruit among citrus genotypes. In regard to carotenoids, such as β-cryptoxanthin, violaxanthin, lycopene, and β-citraurin, the relationship between the carotenoid profile and the expression of carotenoid-biosynthetic genes is discussed. Finally, recent results of quantitative trait locus (QTL) analyses of carotenoid contents and expression levels of carotenoid-biosynthetic genes in citrus fruit are shown. 相似文献
5.
Yukino TAMAMURA-ANDOH Nobuyuki TANAKA Keisuke SATO Yoshino MIZUNO Nobuo ARAI Ayako WATANABE-YANAI Masato AKIBA Masahiro KUSUMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(5):754
We examined the antimicrobial susceptibility of 848 Escherichia coli isolates from 237 feces samples of wild sika deer (Cervus nippon) captured between 2016 and 2019 in 39 of the 47 prefectures of Japan. Five of the 237 wild sika deer (2.1%) carried E. coli with resistance to at least one antimicrobial, and all the resistant isolates showed resistance to tetracycline. The resistant isolates contained antimicrobial resistance genes that were similar to those in E. coli derived from humans and farm animals. Although wild sika deer are not currently likely to be a source for the transmission of antimicrobial resistance in Japan, they can potentially mediate antimicrobial resistance spread by coming into contact with humans, animals, and their surroundings. 相似文献
6.
Hidemasa NAKAMINAMI Yuu OKAMURA Satomi TANAKA Takeaki WAJIMA Nobuo MURAYAMA Norihisa NOGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(2):214
Currently, antimicrobial-resistant staphylococci, particularly methicillin-resistant Staphylococcus pseudintermedius (MRSP), are frequently isolated from canine superficial pyoderma in Japan. However, little is known regarding the nasal prevalence of MRSP in pet dogs. Here, we determined the prevalence of antimicrobial-resistant staphylococci in nares and affected sites of pet dogs with superficial pyoderma. Of the 125 nares and 108 affected sites of pet dogs with superficial pyoderma, 107 (13 species) and 110 (eight species) staphylococci strains, respectively, were isolated. The isolation rate of S. pseudintermedius from pyoderma sites (82/110 strains, 74.5%) was significantly higher than that from nares (57/107 strains, 53.3%) (P<0.01). Notably, the prevalence of MRSP (18/57 strains, 31.6%) in nares was equivalent to that in pyoderma sites (28/82 strains, 34.1%). Furthermore, the phenotypes and genotypes of antimicrobial resistance in MRSP strains from nares were similar to those from pyoderma sites. Our findings revealed that the prevalence of antimicrobial-resistant staphylococci in the nares of pet dogs with superficial pyoderma is the same level as that in affected sites. Therefore, considerable attention should be paid to the antimicrobial resistance of commensal staphylococci in companion animals. 相似文献
7.
Sachiko HYOKAI Hiroyo TANAKA Noriyuki AIHARA Junichi KAMIIE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(3):473
In canine lymphoma, drug resistance is the major factor hindering treatment. In this study, we performed immunohistochemical examination of P-glycoprotein (P-gp) and breast cancer resistance protein (BCRP), which are considered as transporters related to multidrug resistance in three recurrent canine lymphomas. All cases were negative for both transporters before anticancer drug administration, but became positive after this administration. The expression was confirmed in capillary endothelial cells, such as in brain capillaries acting as the blood-brain barrier (BBB). It is suggested that both transporters expressed on capillary endothelial cells in lymphoma tissue may inhibit the spread of anticancer drugs into tumor tissues from blood, the same as the BBB. Therefore, capillary endothelial cells could act as a blood-tumor barrier, which might be involved in drug resistance in canine lymphoma. 相似文献
8.
Natsumi ENDO Larasati Puji RAHAYU Toshiya ARAKAWA Tomomi TANAKA 《The Journal of reproduction and development》2016,62(1):115-119
Here, we report a new method for measuring behavioral patterns during estrus in goats based on video
tracking analysis. Data were collected from cycling goats, which were in estrus (n = 8) or not in estrus (n =
8). An observation pen (2.5 m × 2.5 m) was set up in the corner of the female paddock with one side adjacent
to a male paddock. The positions and movements of goats were tracked every 0.5 sec for 10 min by using a video
tracking software, and the trajectory data were used for the analysis. There were no significant differences
in the durations of standing and walking or the total length of movement. However, the number of approaches to
a male and the duration of staying near the male were higher in goats in estrus than in goats not in estrus.
The proposed evaluation method may be suitable for detailed monitoring of behavioral changes during estrus in
goats. 相似文献
9.
The mammalian uterus changes dramatically during the estrous cycle, pregnancy, and involution post partum. Dynamic changes in the uterine endometrium are a type of homeostasis and proceed with proliferation and exclusion of cells. Homeostasis of the uterus is closely related to apoptosis involving various hormones and cytokines. The objective of the present study was to determine the morphological features and occurrence of apoptosis in the porcine endometrium during the estrous cycle, early pregnancy, and post partum. Cyclic changes in the morphology of the surface epithelium were observed during the estrous cycle. The heights of surface epithelia were significantly high on day 4 of the estrous cycle and the early pregnancy. The heights of the surface epithelium remained low from days 1 to 31 post partum. We then used terminal deoxynucleotidyl transferase-mediated biotinylated deoxyuridine triphosphate nick end-labeling (TUNEL) of the 3'-terminal of fragmented DNA, which is effective for detection of apoptosis in various tissues. We found that apoptosis in the porcine endometrium contributed to homeostasis of the endometrium during the estrous cycle through control of cell proliferation and exclusion. Conversely, apoptosis on days 4 and 8 of gestation before the implantation window depended on the plasma estrogen and progesterone levels; however, suppressive homeostasis of apoptosis occurred at the time of implantation on days 15, 18 and 21 of gestation. Our study is the first to demonstrate apoptotic cell death in the porcine endometrium directly by TUNEL method. The results strongly suggest that uterine homeostasis is mainly controlled by apoptosis during the estrous cycle and early pregnancy. 相似文献
10.
Masaya Nishiyama Ryohei Sugita Shigeto Otsuka Keishi Senoo 《Soil Science and Plant Nutrition》2013,59(5):562-567
Various types of mineral particles in a soil probably provide different microenvironments for microorganisms. The purpose of this study is to investigate whether different types of mineral in a soil harbor different bacterial populations. DNA was extracted from five types (quartz, feldspar, pyroxene, magnetite, iron-coated reddish brown particles) of sand-size mineral particles separated from a sandy soil, and was amplified for partial 16 S rRNA gene by polymerase chain reaction (PCR). Twenty-nine to 69 amplicons per each type of mineral were cloned and sequenced, followed by phylogenetic affiliation of the sequences. As a result, some types of bacteria were detected on all of the types of mineral including the orders Rhizobiales, Bacillales, and Acidobacteriales. In the case of Acidobacteriales, higher percentages were found on magnetite and quartz. Some taxa were restricted to specific types of mineral; the class Actinobacteria was found on pyroxene but not on quartz, and rarely on magnetite and feldspar. Bacterial diversity at the order level estimated by Chao1 value was higher in feldspar and pyroxene than the other three types of mineral. The UniFrac Significance test indicated that the differences in bacterial communitiy structures among the particles were suggestive except that between feldspar and pyroxene. These results support the idea that different communities of bacteria were associated with each of the mineral types. 相似文献