Identification of Aspergillus flavus and aflatoxin in home mix layer poultry feed in relation to seasons in Karachi,Pakistan

Fungal toxins in feed are leading issue in poultry industry causing a detrimental effect on the performance and health of poultry. The study was carried out to determine the incidence and concentration of the aflatoxins and their major producer Aspergillus flavus in home mix layer poultry feed in respect of seasonal variation throughout the year. A total of (n?= 204) home mix poultry layer feed samples were analyzed for the isolation of fungi. The isolates were initially screened through colony morphology and microscopic examination. However, aflatoxin concentration was determined by ELISA. Revealed results indicated that, the highest percentage of A. flavus was found during the months of June to August 50/54 (92.5%) followed by September to November 43/65 (66.1%), March to May 21/40 (52.5%), and December to February 18/45 (40%). As a whole, the incidence was recorded 132/204 (64.7%). Moreover, of the 132 samples, 41 (31%) were exceeded in respect of aflatoxin contamination from the legal limit (20 μg/kg) imposed by Food Drug Association (FDA). Statistically, the growth of A. flavus and aflatoxin production was found significantly different in respect of seasonal variation. As highest total viable fungal count (9.9?×?10⁴ CFU/g) and aflatoxin level (72.27 μg/kg) were recorded during the months of June to August and lowest in December to February. Consequently, instantaneous essential control measures are demanded regarding appropriate storage and adequate drying in post-harvesting season. Along with surveillance plans and austere regulations for monitoring the aflatoxin contents for the wellbeing of consumers.

     

WHEAT RESPONSE TO FARM YARD MANURE AND NITROGEN FERTILIZATION UNDER MOISTURE STRESS CONDITIONS

Information on the combined use of organic and inorganic fertilizers on wheat (Triticum aestivum L.) productivity is lacking under moisture stress conditions of Northwest Pakistan. The present experiment was designed to ascertain the combined effect of organic and inorganic fertilizer management on rainfed wheat. Four levels of farm yard manure, FYM, (0, 10, 20, and 30 Mg FYM ha^?1) and nitrogen (0, 30, 60, 90, and 120 kg N ha^?1) were used. The experiment was conducted at the Agriculture Research Farm of NWFP Agricultural University Peshawar, Pakistan during crop season of 2003–04. The experiment was laid out in randomized complete block design with four replications. Plant height, productive tillers m^?2, grains spike^?1, grain yield, straw yield, and harvest index were significantly higher in plots which received 30 Mg FYM ha^?1. In the case of nitrogen (N) no distinctive differences between the effect of 90 and 120 kg ha^?1 was observed for most of the parameters. Nitrogen application at 90 kg ha^?1 had significantly higher; plant height, grains spike^?1, grain yield, straw yield, and harvest index as compared with the lower levels, i.e., 0, 30, and 60 kg N ha^?1 but were at par with 120 N kg ha^?1. Significantly higher numbers of productive tillers m^?2, grains spike^?1, grain yield, straw yield and harvest index were recorded with application of 30 Mg FYM ha^?1 + 90 kg N ha^?1. The present study suggested that application of 30 Mg FYM ha^?1 + 90 kg N ha^?1 are promising levels for higher production of wheat under moisture stress conditions. Further research work is needed to ascertain the effect of N above 90 kg ha^?1 under different moisture regimes.     

Marine-Derived Biologically Active Compounds for the Potential Treatment of Rheumatoid Arthritis

Rheumatoid arthritis (RA) is a chronic, systemic autoimmune disease with a prevalence rate of up to 1% and is significantly considered a common worldwide public health concern. Commercially, several traditional formulations are available to treat RA to some extent. However, these synthetic compounds exert toxicity and considerable side effects even at lower therapeutic concentrations. Considering the above-mentioned critiques, research is underway around the world in finding and exploiting potential alternatives. For instance, marine-derived biologically active compounds have gained much interest and are thus being extensively utilized to confront the confines of in practice counterparts, which have become ineffective for 21st-century medical settings. The utilization of naturally available bioactive compounds and their derivatives can minimize these synthetic compounds’ problems to treat RA. Several marine-derived compounds exhibit anti-inflammatory and antioxidant properties and can be effectively used for therapeutic purposes against RA. The results of several studies ensured that the extraction of biologically active compounds from marine sources could provide a new and safe source for drug development against RA. Finally, current challenges, gaps, and future perspectives have been included in this review.