Amelioration of a hardsetting Alfisol through deep mouldboard ploughing, gypsum application and double cropping. I. Soil physical and chemical properties

Contrasting soil management techniques were applied to a hardsetting red-brown earth (Alfisol) used for flood-irrigated wheat (Triticum aestivum) production at Trangie, N.S.W., Australia. The individual and combined effects of deep mouldboard ploughing to a depth of 0.45 m, gypsum application (5 t ha⁻¹) and double cropping upon aggregate stability, bulk density, porosity, cone index and the non-limiting water range were evaluated. Dispersion and slaking of the surface soil were unaffected by the treatments when measured at the end of the second year of the experiment. Approximately 60% of the soil mass in the 0–0.15 m layer slaked on wetting, whereas less than 1% of the soil dispersed. Organic carbon (OC) levels of the surface soil were not affected by double cropping or gypsum application, but were reduced by mouldboard ploughing from 0.9% to 0.6% OC. The relationship between OC and macroaggregate (more than 250 μm) stability indicated that large increases in OC beyond 0.7% OC were required for relatively small increases in aggregate stability. Mouldboard ploughing increased clay content of the upper 0.15 m of the soil from 22% to 27%. This was associated with an increase in the frequency and depth of cracking which, however, diminished over time. The non-limiting water range (NLWR) was expanded in the uppermost 0.1 m by gypsum application from 0.15–0.30 to 0.09–0.28 m³ m⁻³. Mouldboard ploughing expanded the NLWR at a depth of 0.2 m. Penetrometer resistance, on average, exceeded the critical value for wheat root growth at a water content of 0.15 m³ m⁻³, which is substantially higher than the wilting point (0.09 m³ m⁻³). Excessive resistance to penetration as opposed to inadequate aeration or water availability is the main agronomic impediment in these soils, at least in the initial stages of crop development. Penetration resistance within the 0.05–0.3 m layer was reduced during a drying cycle in the order: mouldboard ploughing>gypsum>double cropping. The reduced penetration resistance associated with mouldboard ploughing was due to higher water content to a depth of 0.2 m and reduced bulk density below this depth.     

In Vivo and In Vitro Sperm Interaction with Oviductal Epithelial Cells of Llama

Sperm reservoirs in South American Camelids would be crucial for successful fertilization. Since ovulation occurs approximately 36 h after mating, the maintenance of the sperm viability in the oviduct waiting for the ovum is a critical reproductive event. Our study aimed at determining whether the isthmus or the utero tubal junction (UTJ) could function as a sperm reservoir in llama by means of in vivo and in vitro experiments. For the in vivo experiments, the oviducts of adult females with a dominant follicle bigger than 7 mm were examined for the presence of sperm at 6, 18, 24, 28 and 35 h after mating. The results using scanning and transmission electron microscopy showed ultrastructural differences between isthmus and UTJ with respect to (1) predominance of secretory cells in the UTJ and ciliated cells in the isthmus epithelium and (2) cytoplasmic bulbous projection of the secretory cells in the UTJ. Sperm adhered by a mucus‐like substance were seen only in the UTJ at 6, 18, 24 and 28 h post‐mating. Lack of sperm adhered to oviductal mucosa was observed around ovulation (35 h). In vitro experiments demonstrated higher ability of UTJ epithelial cell explants with respect to isthmus explants to bind sperm in a co‐cultured system. The anatomical features and the presence of a sperm bonding agent in the UTJ together with the in vitro differential binding of sperm to UTJ explants strongly suggest that both may be feasible mechanisms that facilitate sperm storage in this oviductal region in llama.     

Cell Counts and Survival to Vitrification of Bovine In Vitro Produced Blastocysts Subjected to Sublethal High Hydrostatic Pressure

This work analyses the effects of a high hydrostatic pressure (HHP) treatment on in vitro survival of in vitro produced (IVP) bovine embryos vitrified with the Cryologic Vitrification Method (CVM). Consequences on embryo quality in terms of cell proliferation and differentiation, and levels of embryonic Heat Shock Protein 70 (Hsp‐70) were also examined. Day 7 and 8 bovine in vitro‐produced blastocysts were submitted to an HHP treatment (60 MPa, at 32°C for 1 h) and allowed to recover for 1 or 2 h in culture medium. The HHP treatment did not improve blastocyst survival rates after vitrification/warming. Survival (24 h post‐warming) and hatching (48 h post‐warming) rates were 79.3 ± 4.9 and 51.8 ± 4.2 vs 73.9 ± 4.2 and 44.7 ± 4.1 for untreated controls and HHP‐treated embryos, respectively. Total cell numbers measured in fresh embryos were reduced after 1 h at 32°C, with or without HHP treatment, indicating that cell proliferation was stopped as a result of stress. Vitrified HHP‐treated embryos that hatched at 48 h after warming showed increased cell numbers in their ICM compared with untreated controls (50.2 ± 3.1 vs 38.8 ± 2.7), indicating higher embryo quality. Treatment of blastocysts with HHP did not alter the level of the Hsp‐70 protein. In our conditions, HHP treatment did not affect the cryoresistance of these embryos. However, combination of HHP treatment and vitrification in fibreplugs resulted in an increase in the ICM cell number of hatched embryos 48 h post‐warming.     

A dielectric omnidirectional reflector

A design criterion that permits truly omnidirectional reflectivity for all polarizations of incident light over a wide selectable range of frequencies was used in fabricating an all-dielectric omnidirectional reflector consisting of multilayer films. The reflector was simply constructed as a stack of nine alternating micrometer-thick layers of polystyrene and tellurium and demonstrates omnidirectional reflection over the wavelength range from 10 to 15 micrometers. Because the omnidirectionality criterion is general, it can be used to design omnidirectional reflectors in many frequency ranges of interest. Potential uses depend on the geometry of the system. For example, coating of an enclosure will result in an optical cavity. A hollow tube will produce a low-loss, broadband waveguide, whereas a planar film could be used as an efficient radiative heat barrier or collector in thermoelectric devices.     

Photoemission evidence for a remnant fermi surface and a d-wave-like dispersion in insulating Ca2CuO2Cl2

An angle-resolved photoemission study is reported on Ca2CuO2Cl2, a parent compound of high-Tc superconductors. Analysis of the electron occupation probability, n(k), from the spectra shows a steep drop in spectral intensity across a contour that is close to the Fermi surface predicted by the band calculation. This analysis reveals a Fermi surface remnant, even though Ca2CuO2Cl2 is a Mott insulator. The lowest energy peak exhibits a dispersion with approximately the &cjs3539;coskxa - coskya&cjs3539; form along this remnant Fermi surface. Together with the data from Dy-doped Bi2Sr2CaCu2O8+delta, these results suggest that this d-wave-like dispersion of the insulator is the underlying reason for the pseudo gap in the underdoped regime.