Suppression of ovarian progesterone production in dairy cows using an implant of GnRH-agonist (deslorelin) for the purpose of evaluating progesterone metabolism

OBJECTIVE: To evaluate the potential of an implant of a GnRH-agonist (deslorelin) to create a progesterone free animal suitable for studying progesterone (P4) metabolism in intact cows by measuring blood P4 and faecal P4 metabolites. METHODS: Experiment 1: Eighteen non-lactating cycling Holstein-Friesian cows, 4 to 7 years old, were allocated to one of three groups to study plasma P4 concentrations preceding an intravaginal insert. These groups comprised: i) a deslorelin group (GnRH-agonist implanted); ii) a PGF group receiving two injections of prostaglandin (PGF2alpha) 12 days apart; and, iii) an ovariectomised (OVX) group. An intravaginal device (CIDR) was inserted into the vagina of each animal and left in place for 11 days. Plasma P4 concentrations were measured during the study period. Experiment 2: Twelve non-lactating cycling Holstein-Friesian cows, 4 to 7 years old, were allocated to two groups: i) a deslorelin group (GnRH-agonist implanted); and ii) an ovariectomised group. Plasma P4 and faecal P4 metabolites (20-oxo-pregnanes, 20alpha-OH and 20beta-OH) were monitored for a period of 5 weeks. RESULTS: Experiment 1: Average plasma P4 concentration did not differ between the three groups (1.28, 1.43 and 1.55 ng/mL for deslorelin, OVX and PGF cows, respectively, P = 0.8) during the period of supplementation. Experiment 2: There was no difference in plasma P4 (mean plasma P4 < 0.02 ng/mL, P = 0.9) and faecal P4 metabolites between deslorelin and OVX cows 2 weeks after the implantation (P = 0.7). CONCLUSIONS: These data showed that a GnRH-agonist (deslorelin) implant may be used as an alternative to ovariectomy to create a progesterone free animal suitable for studying the metabolism of administered P4.     

Detection and genetic diversity of porcine rotavirus A,B and C in eastern Australian piggeries

Rotaviruses (RV) have a high prevalence in piggeries worldwide and are one of the major pathogens causing severe diarrhoea in young pigs. RV species A, B, and C have been linked to piglet diarrhoea in Australian pig herds, but their genetic diversity has not been studied in detail. Based on sequencing of the structural viral protein 7 (VP7) RVA G genotypes G3, G4 and G5, and RVC types G1, G3, G5, and G6 have been identified in Australian piggeries in previous studies. Although occurrence of RVB was reported in Australia in 1988, no further genetic analysis has been conducted. To improve health management decisions in Australian pig herds, more information on RV prevalence and genetic diversity is needed. Here, 243 enteric samples collected from 20 pig farms within Eastern Australia were analysed for the presence of RV in different age groups using a novel PCR-based multiplex assay (Pork MultiPath™ enteric panel). RVA, RVB, and RVC were detected in 10, 14, and 14 farms, respectively. Further sequencing of VP7 in selected RV-positive samples revealed G genotypes G2, G5, G9 (RVA), G6, G8, G14, G16, G20 (RVB), and G1, G3, G5, G6 (RVC) present. RVA was only detected in young (<10 weeks old) pigs whereas RVB and RVC were also detected in older animals (>11 weeks old). Interestingly, RVB and RVC G-type occurrence differed between age groups. In conclusion, this study provides new insights on the prevalence and diversity of different RV species in pig herds of Eastern Australia whilst demonstrating the ability of the Pork MultiPath™ technology to accurately differentiate between these RV species.     

Tibial plateau symmetry and the effect of osteophytosis on tibial plateau angle measurements

A novel technique was developed to estimate the caudal medial tibial plateau landmark in the face of osteophytosis to improve accuracy in tibial plateau angle measurements. Using this technique, tibial plateau angles were evaluated in 31 normal dogs before and 8 months after right cranial cruciate ligament transection. There was no significant difference in mean tibial plateau angle before or after induction of osteophytosis. Additionally, it was determined that 90% of dogs had a difference of =2 degrees between right and left tibial plateau angles, which was considered symmetrical.     

Landscape,Soil, and Plant Analysis of Japanese Stiltgrass (Microstegium vimineum) Invasion in the Piedmont Region,SC

Japanese stiltgrass (Microstegium vimineum), an exotic invasive plant, is native to Southeast Asia. This study was conducted to determine the chemical composition of Japanese stiltgrass as well as soil and landscape characteristics that correlate with invasion of Japanese stiltgrass around Lake Issaqueena in the upper Piedmont of South Carolina. Geographic Information Systems (GISs) and Light Detection and Ranging (LiDAR) were used to determine the spatial pattern of invasion with respect to the aspect, slope, canopy cover, soils, and distance to roads and trails. Japanese stiltgrass was distributed on both sides of Lake Issaqueena in Pacolet and Madison soil map units (Fine, kaolinitic, thermic Typic Kanhapludults) on the average slopes of 21%, but it was particularly common on the eastern shore of the lake in low-lying wet and shaded areas (mean canopy cover 51%). In addition, invasion by Japanese stiltgrass was correlated with the proximity to roads and trails. Plant tissue analysis revealed many differences in the distribution of macronutrients, macrominerals, and micronutrients in the leaves, stems, and roots of Japanese stiltgrass, although those differences were not always statistically significant. Concentrations of nitrogen (N), phosphorus (P), and calcium (Ca) were the highest in leaves while zinc (Zn) concentrations were the highest in stems and concentrations of magnesium (Mg), copper (Cu), manganese (Mn), iron (Fe), and sodium (Na) tended to be higher in roots. Carbon (C), sulfur (S), and potassium (K) concentrations were generally higher in above-ground tissues versus roots. Soil chemical analysis revealed no statistical differences between control and invaded plots. Our findings suggest that watershed areas surrounding lakes may be particularly susceptible to the invasion of Japanese stiltgrass due to their microclimates, low-lying wet pathways for seed distribution and recreational uses.     

Early harvest and ensilage of forage sorghum infected with ergot (Claviceps africana) reduces the risk of livestock poisoning

Sorghum ergot produces dihydroergosine (DHES) and related alkaloids, which cause hyperthermia in cattle. Proportions of infected panicles (grain heads), leaves and stems were determined in two forage sorghum crops extensively infected 2 to 4 weeks prior to sampling and the panicles were assayed for DHES. Composite samples from each crop, plus a third grain variety crop, were coarsely chopped and half of each sealed in plastic buckets for 6 weeks to simulate ensilation. The worst-infected panicles contained up to 55 mg DHES/kg, but dilution reduced average concentrations of DHES in crops to approximately 1 mg/kg, a relatively safe level for cattle. Ensilation significantly (P = 0.043) reduced mean DHES concentrations from 0.85 to 0.46 mg/kg.     

Prevalence of lesions and body condition scores among female swine at slaughter

OBJECTIVE: To determine prevalence of foot lesions, dermatitis, shoulder lesions, mammary gland abnormalities, and visceral lesions, and body condition scores among culled female swine at slaughter. DESIGN: Observational study. ANIMALS: Culled gilts and sows killed during a 1-week period at a Midwest slaughterhouse. PROCEDURE: Carcasses were examined, and lesions were recorded. Body condition was scored on the basis of standard criteria. RESULTS: 58.9% (1,029/1,747) of the carcasses had foot lesions, 67.3% (1,178/1,751) had dermatitis, and 4.6% (80/1,751) had shoulder lesions. Body condition score was significantly associated with detection of dermatitis and shoulder lesions. Mean +/- SE number of teats (n = 1,432 carcasses) was 13.86 +/- 0.02. Mean numbers of normal-appearing teats in the left and right mammary chains were 6.57 +/- 0.02 and 6.58 +/- 0.02, respectively. Feet from 48% (688/1,433) of the carcasses were condemned. Visceral lesions were found in 48.8% (624/1,278) of the carcasses; of the carcasses with lesions, 412 (66%) had liver spots, and 268 (42.9%) had pneumonia. CLINICAL IMPLICATIONS: Lesions that potentially could have adversely affected production were found in a large percentage of culled gilts and sows at slaughter. Knowledge of lesions commonly found at slaughter may help direct changes in herd health programs.