Dual-Energy X-Ray Absorptiometry and Force-Plate Analysis of Gait in Dogs With Healed Femora After Leg-Lengthening Plate Fixation

Dual-energy x-ray absorptiometry was used to measure bone mineral density of four regions in healed femora of nine dogs after fracture fixation with a leg-lengthening plate. Six to 85 months (mean, 46 months) after surgery, the bone mineral density of healed femora was not significantly different from the contralateral uninjured femora ( P >.05; power = 0.8 at Δ= 15%). Radio-lucencies around the proximal screws, apparently associated with screw loosening, were seen on radiographic views of the healed femora of three dogs. In one of these dogs, one screw in the proximal metaphysis had broken. Force-plate analysis of gait was also performed on dogs at the time of bone mineral density measurement. Peak vertical force was decreased in the pelvic limb with the healed fracture compared with the contralateral unoperated limb ( P < 0.05). Clinically apparent lameness in three dogs did not appear to be associated with altered bone mineral density and may have been caused by hip osteoarthritis, a nondisplaced hairline diaphyseal fracture, and screw loosening in conjunction with extensive post-traumatic soft tissue injury.     

Tuberculosis in wild seals and characterisation of the seal bacillus

SUMMARY Tuberculosis was diagnosed in 3 otariid seals found dead on beaches at 3 locations on the south coast of Western Australian between May 1990 and March 1991. This confirms that tuberculosis is present in the 2 native seals (Neophoca cinerea and Arctocephalus forsteri) in Western Australian waters. Mycobacterium sp isolated from the lungs of 2 of the seals were studied to determine the similarity of the strains to each other, to the strains isolated during 1986 from Australian sea lions and New Zealand fur seals kept in captivity at a marine park near Perth, Western Australia, and to a strain isolated in 1988 from a seal trainer who worked with the infected captive seals for 3 years. After restriction endonuclease analysis (REA) with the endonucleases Bst Ell, Bcl I and Pvu II, one of the wild seal strains appeared to have identical DNA fragment patterns to the strains from the captive seals and the seal trainer. The other wild seal isolate had identical REA profiles using Bst EII and Bcl I, but a minor difference was detected using Pvu II. Differences in these isolates were more clearly seen in restriction fragment length polymorphisms after hybridisation with two DNA probes. The secretory protein MPB70, present in M bovis, was not detected in wild seal isolates using sodium dodecyl sulphate polyacrylamide gel electrophoresis and Western blotting techniques. Analysis of protein and DNA fragment profiles indicated that seal tuberculosis isolates form a unique cluster within the M tuberculosis complex.     

A Comparison of Injectable Anesthetic Combinations in Horses

Six combinations of injectable anesthetic agents were administered to six adult horses in a Latin square design. The drug combinations were xylazine-ketamine, xylazine-butorphanol-ketamine, xylazine-tiletamine-zolazepam, xylazine-butorphanol-tiletamine-zolazepam, detomidine-ketamine, and detomidine-butorphanol-ketamine. Measured variables were heart rate, respiratory rate, systolic blood pressure, arterial pH (pHa), PaCO2, PaO2, recumbency time, and number of attempts necessary to stand. Quality of induction and recovery, muscle relaxation, and response to stimulus were evaluated subjectively. The horses required significantly more attempts to stand after administration of xylazine-tiletamine-zolazepam, xylazine-butorphanol-tiletamine-zolazepam, and detomidine-ketamine than after xylazine-ketamine, xylazine-butorphanol-ketamine, or detomidine-butorphanol-ketamine. Mean recumbency times varied from 23.0 minutes with xylazine-ketamine to 41.3 minutes with xylazine-butorphanol-tiletamine-zolazepam. There were significant differences in mean heart rates at minute 15, mean respiratory rates at minutes 5, 10 and 15, and mean systolic blood pressures at minute 10 of anesthesia. There were no significant differences in pHa, PaCO2 or PaO2.     

ADSORPTION AND DESORPTION OF SIMAZINE BY SOME ROTHAMSTED SOILS

Summary. The adsorption of simazine from, and subsequent desorption into, 0–01 M calcium chloride solution was investigated using twenty-three Rothamsted soil samples from sites differing greatly in cropping history and manurial treatment. Organic carbon content was the only factor related to the ability of the soils to sorb simazine; this accounted for 90% or more of the variation between soils Equilibrium was attained during adsorption in from fewer than 2 to more than 24 hr. Equilibrium during desorption was only occasionally attained within 24 hr. Differences between theoretically predicted and measured concentrations of simazine in solution following desorption were least for soils that attained equilibrium fastest during adsorption. Differences in adsorption and desorption kinetics between soils could not be related to soil pH, organic carbon content or cropping and manuring history. Comparisons of unlimed and limed soils suggested that no simnazine was lost by acid hydrolysis during the experiments.
Adsorption et désorption de la simazine par quelques sols de Rothamsted     

The influence of temperature and photoperiod on growth and development of Parthenium hysterophorus L.

Germination, growth and flowering were investigated in Parthenium hysterophoms (Asteraceae), a species introduced to Australia and at present occurring as a weed in central Queensland. Combinations of day/night temperature regimes from 15/4 to 36/31 and day length of 10, 13 and 16 h in controlled conditions were examined to find the effects of any environmental restraints to growth and development. Results showed that individuals are able to germinate, grow and flower over a wide range of temperatures and photoperiods and established plants can survive at least one mild frost of — 2. We conclude that the potential distribution of P. hyslerophorus in Australia is much wider than at present. Future distribution of the species may be limited seasonally by the inability of seed to germinate in soils of low water potential and by the inability of seedlings to establish and grow at low light intensities.     

Apparent digestible protein,energy and amino acid availability of three plant proteins in Florida pompano,Trachinotus carolinus L. in seawater and low‐salinity water

Two experiments were conducted with Florida pompano, Trachinotus carolinus L. at 3 and 28 g L^?1 salinity to determine apparent crude protein digestibility (ACPD), energy digestibility (AED) and amino acid availability (AAAA) from soybean meal (SBM), soy protein isolate (SPI) and corn gluten meal (CGM). Mean AAAA was similar to ACPD. In fish adapted to 3 g L^?1 salinity, they were 81.2% and 81.9% (CGM), 93.6% and 92.2% (SBM), 93.8% and 93.1% (SPI) for AAAA and ACPD respectively. In fish adapted to 28 g L^?1, they were 84.5% and 83.4% (CGM), 86.5% and 87.1% (SBM), and 83.4% and 85.0% (SPI) for AAAA and ACPD respectively. The AED was highest for SPI and lowest for SBM and inversely related to carbohydrate. The ACPD, AED and AAAA of soy products appeared to be lower in high salinity, whereas CGM was unaffected. The data suggest that SBM, SPI and CGM should be further evaluated as partial fishmeal replacements in Florida pompano diets. Application of the generated coefficients can be used to develop well‐balanced, low‐cost diets for Florida pompano reared in low salinity or seawater.     

CHANGES IN MOLECULAR WEIGHT DISTRIBUTION OF SOIL ORGANIC MATTER DURING SOIL DEVELOPMENT

Ten different topsoils representing members of three soil chronosequences were pre-treated with 0.1 M HC1 and extracted with neutral 0.1 M Na₄P₂O₇ followed by 0.5 M NaOH. Extracts were purified and fractionated into five different nominal molecular weight fractions using gel filtration. With increasing soil development, the proportion of large molecules (>200 000) in the organic matter decreased with an increase in the proportion of intermediate size molecules (200 000 – 10 000). This effect was more evident in the mild Na₄P₂O₇ extracts than in the subsequent NaOH extracts, and in soils of the Manawatu chronosequence than in those of the other two chronosequences and are attributed to changes in the nature and humification of soil organic matter during soil development.     

Forecasting the national incidence of sugar-beet powdery mildew from weather data in Britain

In a national survey of the incidence of powdery mildew (Erysiphe betae) in sugar beet in Britain, between 1980 and 1989, in which between 335 and 717 fields were inspected at the end of August each year, the average crop area infected ranged from 2% in 1986 to 68% in 1989. The effect of various climatic factors that might be expected to influence powdery mildew development over this period was examined, using meteorological records from Broom's Barn Experimental Station, Using simple linear regression analyses, a significant association was found between disease incidence and both air temperature and rainfall incidence in the period April-August, However, air temperature and frost incidence in some winter months were also closely associated with the incidence of powdery mildew in the following crop.
Combinations of these variables were examined in stepwise multiple-regression analyses, The model of best fit, incorporating winter frost incidence with summer temperature and rainfall incidence, accounted for 95% of the variation. Model validation procedures demonstrated the superiority of this model over those involving single variables. Nevertheless, for forecasting purposes, the best correlation was found between the crop area infected at the end of August each year and the number of days with a ground frost in the preceding February and March. This single climatic variable accounted for 82% of the variation and its potential use to forecast powdery mildew incidence in the national sugar-beet crop is discussed.