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排序方式: 共有430条查询结果,搜索用时 15 毫秒
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Removal of hemangiosarcoma cells from canine blood with a cell salvage system and leukocyte reduction filter 下载免费PDF全文
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The 5‐lipoxygenase inhibitor tepoxalin induces oxidative damage and altered PTEN status prior to apoptosis in canine osteosarcoma cell lines 下载免费PDF全文
J. P. Loftus D. Cavatorta J. J. Bushey C. B. Levine C. S. Sevier J. J. Wakshlag 《Veterinary and comparative oncology》2016,14(2):e17-e30
The 5‐lipoxygenase (5‐LOX) inhibitor tepoxalin has been shown to slow canine osteosarcoma (OSA) tumour xenografts growth, yet the mechanisms are poorly elucidated. Further examination of tepoxalin in canine OSA cell lines shows that tepoxalin treated cells undergo apoptosis through caspase‐3 activation and annexin staining. Interestingly, apoptosis is superseded by an increase in reactive oxygen species (ROS), as measured by activation of dihydrorhodamine 123 and mitosox. This increase in ROS appears to be related to the 5‐LOX inhibitor regardless of cellular 5‐LOX status, and was not observed after treatment with the tepoxalin metabolite RWJ20142. Additionally, 5‐LOX inhibition by tepoxalin appears to increase phosphatase and tensin (PTEN) homolog activity by preventing its alkylation or oxidation. PTEN modification or inhibition allows phosphoinositide‐3 (PI3) kinase activity thereby heightening activation of protein kinase B (AKT) phosphorylation. Our data suggest that off target oxidation and LOX inhibition play roles in the apoptotic response. 相似文献
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A comparison of clinical,magnetic resonance imaging and pathological findings in dogs with gliomatosis cerebri,focusing on cases with minimal magnetic resonance imaging changes‡ 下载免费PDF全文
R. T. Bentley G. N. Burcham H. G. Heng J. M. Levine R. Longshore S. Carrera‐Justiz K. Kopf M. A. Miller 《Veterinary and comparative oncology》2016,14(3):318-330
The primary study objective was to determine whether clinical examination and magnetic resonance imaging (MRI) can underestimate canine gliomatosis cerebri (GC); we also investigated immunohistochemical features. Seven dogs with GC were studied; four recruited specifically because of minimal MRI changes. Neuroanatomic localization and the distribution of MRI, gross and sub‐gross lesions were compared with the actual histological distribution of neoplastic cells. In six cases, clinical examination predicted focal disease and MRI demonstrated a single lesion or appeared normal. Neoplastic cells infiltrated many regions deemed normal by clinical examination and MRI, and were Olig2‐positive and glial fibrillary acid protein‐negative. Four dogs had concurrent gliomas. GC is a differential diagnosis for dogs with focal neurological deficits and a normal MRI or a focal MRI lesion. Canine GC is probably mainly oligodendrocytic. Type II GC, a solid glioma accompanying diffuse central nervous system neoplastic infiltration, occurs in dogs as in people. 相似文献
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Retrospective Evaluation of Horses Diagnosed with Neuroborreliosis on Postmortem Examination: 16 Cases (2004–2015) 下载免费PDF全文
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Experimental Neospora Caninum Infection in Pregnant Dairy Heifers Raises Concentrations of Pregnancy‐Associated Glycoproteins 1 and 2 in Foetal Fluids 下载免费PDF全文
R Mur‐Novales F López‐Gatius B Serrano‐Pérez I García‐Ispierto L Darwich O Cabezón NM de Sousa JF Beckers S Almería 《Reproduction in domestic animals》2016,51(2):282-286
Plasma concentrations of PAG‐1 are used for pregnancy diagnosis and as a marker of placental/foetal well‐being, while those of PAG‐2 may be an indicator of abortion risk in Neospora caninum‐infected cows. Studies have shown that N. caninum infection modifies PAG‐1 and PAG‐2 patterns in maternal blood plasma. However, no prior work has examined the effects of N. caninum infection on concentrations of PAGs in foetal fluids. In this study, PAG‐1, PAG‐2 and pH levels were determined in the amniotic and allantoic fluids of foetuses collected at 152 days of gestation from control uninfected dams and from dams experimentally infected with N. caninum on Day 110 of gestation. Foetal fluids from infected foetuses had significantly higher PAG‐2 concentrations (p = 0.026) and pH values (p = 0.02) than fluids from non‐infected foetuses. In infected foetuses, significantly higher concentrations of PAG‐1 (p < 0.001) and PAG‐2 (p < 0.001) were detected in fluid samples showing antibodies against N. caninum than those without antibodies. Moreover, pH values were significantly higher (p = 0.011) in foetal fluid samples with antibodies than in samples from non‐infected foetuses. In conclusion, this is the first report on the effect of N. caninum infection on PAG levels in foetal fluids. Our results indicate that following the experimental infection of dams with N. caninum on Day 110 of gestation, foetal fluids collected from the infected foetuses of these dams featured higher PAG‐1 and PAG‐2 levels and pH values than fluids from non‐infected controls, provided that the samples tested showed the presence of antibodies. The clinical implications of these findings are that following infection with N. caninum, most cows will experience some level of placental damage and that this injury correlates with foetal fluid PAG levels and pH. 相似文献
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Theory of mind (ToM) to infer other people's current mental states and episodic memory of personal happenings have been assumed to be closely related. We report two participants with severely impaired episodic memory who perform indistinguishably from healthy controls on objective ToM tests. These results suggest that ToM can function independently of episodic memory. 相似文献
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Levine DG Smith JJ Richardson DW Brown V Beech J Habecker P Adam E 《Journal of the American Veterinary Medical Association》2007,231(3):437-441
CASE DESCRIPTION: A 6-month-old male Bactrian camel was examined because of a 3-week history of lameness of the left hind limb. CLINICAL FINDINGS: Lameness was initially detected in the left hind limb but resolved and was detected in the right hind limb during treatment. Lameness increased during periods of rapid growth. Radiography revealed multiple small opacities of the medullary cavity of several long bones throughout treatment. Core bone biopsies of lesions in the tibiae revealed lamellar bone with areas of loose connective tissue, osteoblasts in the medullary cavity, and periosteal new bone formation, all which were consistent with panosteitis. TREATMENT AND OUTCOME: Palliative treatment was attempted with epidural and transdermal administration of analgesics. Flunixin meglumine was administered PO, which coincided with an abrupt increase in serum creatinine concentration. Performance of multiple diagnostic bone biopsies led to remission of clinical signs of pain. CLINICAL RELEVANCE: Panosteitis should be a differential diagnosis for shifting limb lameness in young camels. Bone biopsies can be useful for diagnosis of panosteitis and possible relief of pain associated with the disease. Bactrian camels may be susceptible to the renal toxicity of flunixin meglumine, especially when dehydrated. 相似文献
10.
This paper attempts to identify the critical elements associated with the introduction and management of the practice of Rotational Irrigation in Taiwan, and thus to shed some light on the basic requirements for its successful adoption. While the emphasis is on this specific practice we believe it has implications for the transfer of other technologies in which physical, biological and managerial factors interact importantly.Seven irrigation associations in Taiwan were studied to identify the external conditions and system characteristics that are necessary for a successful change from continuous to rotational irrigation. Three external essentials are identified: (1) a water deficit approximating 25 percent of normal practice in the area; (2) political commitment to the change; (3) adequate financial resources for implementation and subsequent operation. Two system characteristics considered essential are: (1) physical facilities that provide for control and measurement to the level of farmer group responsibility; and (2) management patterns that provide a well-organized operating group and effective communication between the farmers and system personnel, resulting in a responsive plan of operation.Specific adaptations of the Taiwan practices to the local conditions will be necessary before effective transfer of this technology can take place elsewhere. 相似文献