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1.
Soil amidase activity in polyacrylamide-treated soils and potential activity toward common amide-containing pesticides 总被引:1,自引:0,他引:1
Jeanine L. Kay-Shoemake M. E. Watwood Robert E. Sojka Roderick D. Lentz 《Biology and Fertility of Soils》2000,31(2):183-186
Polyacrylamide (PAM) is currently used as an irrigation water additive to significantly reduce the amount of soil erosion
that occurs during furrow irrigation of crops. Elevated soil amidase activity specific toward the large PAM polymer has been
reported in PAM-treated field soils; the substrate specificity of the induced amidase is uncertain. PAM-treated and untreated
soils were assayed for their capacity to hydrolyze the amide bond in carbaryl (Sevin), diphenamid (Dymid), and naphthalene
acetamide. Based on results obtained with a soil amidase assay, there was no difference between PAM-treated and untreated
soils with respect to the rate of amide bond hydrolysis of any of the agrochemicals tested. It appears that under these assay
conditions the PAM-induced soil amidase is not active toward the amide bonds within these molecules. However, carbaryl was
hydrolyzed by a different soil amidase. To our knowledge, this is the first soil enzyme assay-based demonstration of the hydrolysis
of carbaryl by a soil amidase.
Received: 23 June 1999 相似文献
2.
Lentz LR Valberg SJ Herold LV Onan GW Mickelson JR Gallant EM 《American journal of veterinary research》2002,63(12):1724-1731
OBJECTIVE: To determine whether alterations in myoplasmic calcium regulation can be identified in muscle cell cultures (myotubes) and intact muscle fiber bundles derived from Thoroughbreds affected with recurrent exertional rhabdomyolysis (RER). ANIMALS: 6 related Thoroughbreds with RER and 8 clinically normal (control) Thoroughbred or crossbred horses. PROCEDURES: Myotube cell cultures were grown from satellite cells obtained from muscle biopsy specimens of RER-affected and control horses. Fura-2 fluorescence was used to measure resting myoplasmic calcium concentration as well as caffeine- and 4-chloro-m-cresol (4-CMC)-induced increases in myoplasmic calcium. In addition, intact intercostal muscle fiber bundles were prepared from both types of horses, and their sensitivities to caffeine- and 4-CMC-induced contractures were determined. RESULTS: Myotubes of RER-affected and control horses had identical resting myoplasmic calcium concentrations. Myotubes from RER-affected horses had significantly higher myoplasmic calcium concentrations than myotubes from control horses following the addition of > or = 2mM caffeine; however, there was no difference in their response to 4-CMC (> or = 1 mM). Caffeine contracture thresholds for RER and control intact muscle cell bundles (2 vs 10mM, respectively) were significantly different, but 4-CMC contracture thresholds of muscle bundles from RER-affected and control horses (500 microM) did not differ. CONCLUSIONS AND CLINICAL RELEVANCE: An increase in caffeine sensitivity of muscle cells derived from a family of related RER-affected horses was detected in vitro by use of cell culture with calcium imaging and by use of fiber bundle contractility techniques. An alteration in muscle cell calcium regulation is a primary factor in the cause of this heritable myopathy. 相似文献
3.
By convening a multidisciplinary team(the Monitoring Team) that included farmers,university and agency researchers, andnon-profit staff; a small group of farmers insoutheast Minnesota, U.S.A., bolstered thelegitimacy of the sustainable agriculturemovement. Through the experience of forming ateam and working with individuals who operatedwithin the mainstream knowledge paradigm,farmers gained validation of their knowledgeabout farming, while researchers came to valuealternative knowledge systems. In the contextof a socially embedded movement, farmers wereempowered by sharing their knowledge withresearchers, and ultimately contributed to thesustainable agriculture movement by challengingtraditional patterns of knowledge exchange. 相似文献
4.
Bright Spots, Structure, and Magmatism in Southern Tibet from INDEPTH Seismic Reflection Profiling 总被引:8,自引:0,他引:8
LD Brown W Zhao KD Nelson M Hauck D Alsdorf A Ross M Cogan M Clark X Liu J Che 《Science (New York, N.Y.)》1996,274(5293):1688-1690
INDEPTH seismic reflection profiling shows that the decollement beneath which Indian lithosphere underthrusts the Himalaya extends at least 225 kilometers north of the Himalayan deformation front to a depth of approximately 50 kilometers. Prominent reflections appear at depths of 15 to 18 kilometers near where the decollement reflector apparently terminates. These reflections extend north of the Zangbo suture to the Damxung graben of the Tibet Plateau. Some of these reflections have locally anomalous amplitudes (bright spots) and coincident negative polarities implying that they are produced by fluids in the crust. The presence of geothermal activity and high heat flow in the regions of these reflections and the tectonic setting suggest that the bright spots mark granitic magmas derived by partial melting of the tectonically thickened crust. 相似文献
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Extract Myophosphorylase deficiency (glycogen storage disease Type V) has been diagnosed in Charolais calves in New Zealand. It is manifested as exercise intolerance and recumbency, usually at several weeks of age, due to an inability to mobilise glucose, as glucose phosphate, from glycogen. Affected calves may rise and walk after a rest to again become affected on further exercise. There is rhabdomyolysis and after repeated or severe attacks the calf may remain recumbent and need to be euthanised. The mutant phosphorylase gene has been described. We have established a PCR-RFLP test based on a published method, and have confirmed the diagnosis in affected calves and heterozygous status of sires, dams and other related individuals belonging to an extended family. In addition, several other non-related heterozygotes have been identified in that and another herd. There appears to have been importations of the gene from both England and America. 相似文献
8.
AC Johnstone KD McSporran JE Kenny IL Anderson GR MacPherson RD Jolly 《New Zealand veterinary journal》2013,61(6):404-408
AIM: To describe a disease of muscle in Charolais calves and confirm the putative diagnosis of inherited myophosphorylase deficiency. METHODS: Variously stained paraffin sections of muscle prepared from affected calves were used to describe the lesions. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) test was developed and applied to affected calves, their sires, dams and other individuals. RESULTS: The lesions were those of rhabdomyolysis of skeletal muscles and sub-sarcolemmal spaces in normal fibres. The PCR-RFLP test confirmed the expected mutation for phosphorylase deficiency of Charolais cattle in two affected calves. In addition, sires, dams and other closely-related individuals of four affected calves tested as heterozygous for the mutation. Other apparently unrelated animals also tested as heterozygous. CONCLUSIONS: The diagnosis of myophosphorylase deficiency was confirmed. The PCR-RFLP test is suitable for use in controlling this recessively-inherited disorder as it can diagnose heterozygous individuals that are otherwise clinically normal. 相似文献
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10.
Visible quantum cutting in LiGdF4:Eu3+ through downconversion 总被引:1,自引:0,他引:1
For mercury-free fluorescent lamps and plasma display panels, alternative luminescent materials are required for the efficient conversion of vacuum ultraviolet radiation to visible light. Quantum cutting involving the emission of two visible photons for each vacuum ultraviolet photon absorbed is demonstrated in Eu3+-doped LiGdF4 with the concept of downconversion. Upon excitation of Gd3+ with a high-energy photon, two visible photons can be emitted by Eu3+ through an efficient two-step energy transfer from Gd3+ to Eu3+, with a quantum efficiency that approaches 200 percent. 相似文献