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Measurements of movement along 28 boreholes reveal the three-dimensional flow field in a 6 million cubic meter reach of Worthington Glacier, a temperate valley glacier located in Alaska. Sliding at the bed accounted for 60 to 70 percent of the glacier's surface motion. Strain rates in the ice were low from the surface to a depth of about 120 meters, but then increased rapidly toward the bed. Ice deformation was not affected by temporal changes in the sliding rate. The three-dimensional pattern of motion indicates that plane strain, which is often assumed by models, is a poor approximation of this viscous flow. 相似文献
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P. J. Bergman D. M. Craft S. J. Newman K. Baer M. A. CampsPalau J. A. McKnight N. F. Leibman S. Brenn K. Finora A. E. Hohenhaus T. Bartick A. K. Patnaik T. Scase 《Veterinary and comparative oncology》2004,2(2):98-98
Introduction: The Patnaik grading system for canine cutaneous MCT is currently one of the best determinants of prognosis; however, clinical outcome does not always correlate with histologic grade. The development of molecular markers offers a potential advantage and may complement subjective grading. The primary purpose of this study was to correlate histologic grading to Ki67/PCNA/AgNOR/c‐Kit scores. Methods: Thirty‐eight dogs with cutaneous MCT underwent surgical resection. Tumors were graded, with expansion of grade II MCT to low, medium (or II only) and high. For statistical purposes, MCT grade I, II (low, medium, high) and III were assigned a score of 1, 2, 3, 4, or 5, respectively. Sections were processed for AgNOR staining and expression of PCNA, Ki67 and c‐Kit as previously described (modified biotin‐strepavidin with DAB substrate). Paraffin‐embedded canine tissue arrays were used as positive and negative controls (primary antibody replaced with pre‐immune sera). Parametric statistical testing was performed using Statview statistical software with P ≤ .05 as significant. Results: There were 12, 20, 5 and 1 grade II low, grade II medium, grade II high and grade III MCT, respectively. The mean Ki67 score was 9.114 (median 8.0, range 1–28), mean PCNA score was 26.25 (median 24.0, range 5–65), mean AgNOR score was 1.499 (median 1.35, range 1.02–2.76) and c‐Kit scores were +1 (9/37), +2 (19/37) and +3 (9/37). With parametric statistical testing, significantly positive correlations were found for Ki67/Grade, PCNA/Grade, AgNOR/Grade, Ki67/PCNA, Ki67/AgNOR and PCNA/AgNOR (all P < .0001). No significant correlation was found for c‐Kit and grade; however, +3 c‐Kit scores had statistically significantly higher grades than +2 c‐Kit scores (P = .0458). Conclusions: Ki67/PCNA/AgNOR scores are positively correlated to grade in dogs with MCT. Further studies to correlate Ki67/PCNA/AgNOR/c‐Kit scores with clinical variables are ongoing. 相似文献
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S. C. Charney P. J. Bergman J. A. McKnight J. Farrelly C. A. Novosad N. F. Leibman M. A. Camps‐Palau 《Veterinary and comparative oncology》2005,3(4):171-181
Carcinomatosis, sarcomatosis and mesothelioma, with or without malignant effusions, are difficult to treat and generally carry a poor prognosis. The purpose of this study was two‐fold; first, to determine the prognosis for dogs with carcinomatosis, sarcomatosis, or mesothelioma, with or without malignant effusions; second, to evaluate the safety and efficacy of treatment with intracavitary (IC) carboplatin and mitoxantrone in dogs with these syndromes. Nineteen dogs were evaluated. Seven were untreated and 12 were treated with IC chemotherapy (mitoxantrone and/or carboplatin), and multiple factors were analysed for significance with respect to survival time. The median survival time (MST) for untreated dogs was 25 days, whereas the MST for treated dogs was 332 days (Log Rank, P < 0.0001). Treatment with IC chemotherapy was well tolerated. This study suggests that IC chemotherapy with mitoxantrone and/or carboplatin is an effective treatment for dogs with carcinomatosis, sarcomatosis or mesothelioma, with or without malignant effusion. 相似文献
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K. Finora N. F. Leibman M. J. Fettman B. E. Powers T. A. Hackett S. J. Withrow 《Veterinary and comparative oncology》2006,4(3):178-183
Staging of dogs with cutaneous mast cell tumours (MCTs) is an important diagnostic step. Aspiration of the liver and the spleen is often part of routine staging. This study cytologically compared mast cell numbers in fine‐needle aspirates of liver and spleen of clinically normal unaffected dogs with those of dogs with cutaneous MCT and an ultrasonographically normal appearing liver and spleen. The unaffected dogs (n = 32) were selected from humane society dogs, and the affected dogs (n = 51) were selected from hospital cases. There were no statistically significant differences in each of the parameters evaluated for the liver aspirates. For splenic aspirates, affected dogs showed significantly more mast cells per cluster (P = 0.04) and more isolated mast cells per slide (P = 0.03) compared with unaffected dogs. However, no clinically important difference existed between the unaffected and affected dogs; thus, routine aspiration of an ultrasonographically normal appearing liver and spleen of dogs with cutaneous MCT does not appear to be a clinically useful staging tool. 相似文献
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Diana Leibman Nelia Ortega-Parra Dalia Wolf Meital Shterkman Inge Hanssen Amit Gal-On 《Plant pathology》2021,70(4):1003-1012
RNA interference (RNAi) or gene silencing is a natural defence response of plants to invading viruses. Here, we applied this approach against pepino mosaic virus (PepMV) isolates in their natural host, tomato. PepMV isolates differ in their genetic sequences, the severity of the disease they induce, and their worldwide distribution. PepMV causes heavy crop losses, mainly due to impaired tomato fruit quality. Resistant varieties are not yet available, despite many years of resistance breeding efforts within the tomato seed industry. To generate broad resistance to PepMV strains, conserved sequences from three different strains of PepMV (US1, LP, and CH2) were synthesized as a single insert and cloned in a hairpin configuration into a binary vector, which was used to transform tomato plants. Transgenic tomato lines that expressed a high level of transgene-siRNA exhibited immunity to PepMV strains, including a new Israeli isolate. This immunity was maintained even after graft inoculation, in which a transgenic scion was grafted onto nontransgenic infected rootstocks. However, an immune transgenic rootstock was unable to induce resistance in a nontransformed scion. These results provide the first example of engineered immunity to diverse PepMV strains in transgenic tomato based on gene silencing. 相似文献
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Victor Gaba Arie Rosner Ludmilla Maslenin Diana Leibman Sima Singer Emre Kukurt Yoel M. Shiboleth Amit Gal-On 《European journal of plant pathology / European Foundation for Plant Pathology》2010,128(2):153-164
Virus resistance can be effectively generated in transgenic plants by using the plant’s silencing machinery. To study the
specificity of gene-silencing-based resistance, homozygous tobacco (Nicotiana tabacum L.) plants containing a 597-nt hairpin RNA construct of the Potato Virus Y (PVY) replicase sequence were challenged with a variety of PVY strains. The transgene-carrying tobacco line was immune to
five potato PVY strains with high sequence similarity (88.3–99.5%) to the transgene. Infection with more distant tomato and
pepper PVY field strains (86–86.8% sequence similarity) caused delayed symptom appearance in the transgenic tobacco. Transgene
production of small interfering (si) RNA was detected by northern blot and measured using a custom-designed microarray for
the detection of small RNAs. siRNA accumulation peaks were observed throughout the inverted-repeat transgene. In the resistance-breaking
tomato and pepper strains there were nucleotide differences in the sequences correlated to siRNA transgene accumulation, indicating
the role of siRNA specificity in resistance breaking. The log of transgene siRNA signal intensity increased with probe GC
content, indicating that the accumulating siRNA molecules were GC-rich. Sequence similarity of highly accumulating siRNAs
with the target virus strain appears to be important for both resistance and resistance-breaking characteristics. 相似文献
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Magnetic Field and Plasma Observations at Mars: Initial Results of the Mars Global Surveyor Mission 总被引:2,自引:0,他引:2
MH Acu?a JEP Connerney P Wasilewski RP Lin KA Anderson CW Carlson J McFadden DW Curtis D Mitchell H Reme C Mazelle JA Sauvaud C d'Uston A Cros JL Medale SJ Bauer P Cloutier M Mayhew D Winterhalter NF Ness 《Science (New York, N.Y.)》1998,279(5357):1676-1680
The magnetometer and electron reflectometer investigation (MAG/ER) on the Mars Global Surveyor spacecraft has obtained magnetic field and plasma observations throughout the near-Mars environment, from beyond the influence of Mars to just above the surface (at an altitude of approximately 100 kilometers). The solar wind interaction with Mars is in many ways similar to that at Venus and at an active comet, that is, primarily an ionospheric-atmospheric interaction. No significant planetary magnetic field of global scale has been detected to date (<2 x 10(21) Gauss-cubic centimeter), but here the discovery of multiple magnetic anomalies of small spatial scale in the crust of Mars is reported. 相似文献
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NP STEWART NF STANDFAST† FC BALDOCK‡ DJ REID AJ de VOS† 《Australian veterinary journal》1992,69(3):59-61
The distribution and prevalence of Thelleria buffeli in Queensland cattle were investigated using serum samples and blood films collected primarily for brucellosis surveillance and tick fever diagnosis. Serums from 8654 cattle from 357 farms throughout Queensland were examined by an indirect fluorescent antibody test for antibody to T buffeli. In addition, 347 peripheral blood films collected from 147 farms in south-eastern Queensland were examined for piroplasms of T buffeli. The overall herd and animal prevalences for T buffeli were 75% and 41%, respectively. There was significant variation among regions in both herd and animal prevalences (P less than 0.001). Herd and animal prevalences were highest in the north and east decreasing westward. The results indicate that T buffeli is more widespread in Queensland than previously thought. 相似文献