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1.
Benny J. Woody DVM MS Michael J. Murphy DVM PhD AIlen C. Ray PhD Robert A. Green DVM PhD 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1992,6(1):23-28
The clinical signs and laboratory changes of brodifacoum (BDF) intoxicated dogs and their response to vitamin K1 treatment were examined. Brodifacoum, a second-generation anticoagulant rodenticide, was fed to four dogs for 3 consecutive days producing a cumulative dose of 1.1 mg BDF/kg body weight. Clinical observations of the animals were made daily throughout the study. Monitored laboratory parameters included: one-stage prothrombin time (OSPT), activated partial thromboplastin time (APTT), activated coagulation time (ACT), complete blood counts, thrombocyte counts, and serum chemistry values. Response to vitamin K1 therapy was evaluated clinically and by laboratory tests. Serum BDF concentrations were monitored. Inappetence and hemorrhagic tendencies were exhibited by day 5 postrodenticide exposure. One-stage prothrombin time, APTT, and ACT were 25% greater than time zero values at 24, 24, and 72 hours postdosing, respectively. All laboratory parameters returned to normal within 48 hours of initiating vitamin K1 therapy (0.83 mg/kg orally, TID for 5 days). Serum brodifacoum concentrations were highest (1065-1215 ng/mL) during the 3 days after BDF dosing and were detectable (3.0-7.5 ng/mL) until day 24 postexposure. A mean BDF elimination half-life of 6 +/- 4 days was observed. 相似文献
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Natoli E Maragliano L Cariola G Faini A Bonanni R Cafazzo S Fantini C 《Preventive veterinary medicine》2006,77(3-4):180-185
In Italy, which is rabies-free, the national Law No. 281 [Legge Nazionale 14 agosto 1991. No. 281: Legge Quadro in materia di animali di affezione e prevenzione del randagismo. Gazz. Uff. Rep. Ital. no 203 del 30 agosto 1991: p. 3] on the management of pets and on the control of feral cats has introduced the no-kill policy for this species. Thus, “trap-neuter-release” (TNR) programs have been carried out for >10 years. In this paper we present data on registered colonies and censused cats in Rome from 1991 to 2000; the results of the neutering campaign from 1991 to 2000; and a survey, on 103 cat colonies, on the effects of demographic control of urban feral-cat colonies in the city of Rome, carried out by the local Veterinary Public Services (VPS) in collaboration with the associations of cat care-takers. In 10 years almost 8000 were neutered and reintroduced in their original colony. The spay/neuter campaigns brought about a general decrease in cat number but the percentage of cat immigration (due to abandonment and spontaneous arrival) is around 21%. This suggests that all these efforts without an effective education of people to control the reproduction of house cats (as a prevention for abandonment) are a waste of money, time and energy. 相似文献
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Kaiping Deng Steffen Uhlig Laura B. Goodman Hon S. Ip Mary Lea Killian Sarah M. Nemser Jodie Ulaszek Shannon Kiener Matthew Kmet Kirstin Frost Karina Hettwer Bertrand Colson Kapil Nichani Anja Schlierf Andriy Tkachenko Mothomang Mlalazi-Oyinloye Andrew Scott Ravinder Reddy Gregory H. Tyson 《Journal of veterinary diagnostic investigation》2022,34(5):825
The COVID-19 pandemic presents a continued public health challenge. Veterinary diagnostic laboratories in the United States use RT-rtPCR for animal testing, and many laboratories are certified for testing human samples; hence, ensuring that laboratories have sensitive and specific SARS-CoV2 testing methods is a critical component of the pandemic response. In 2020, the FDA Veterinary Laboratory Investigation and Response Network (Vet-LIRN) led an interlaboratory comparison (ILC1) to help laboratories evaluate their existing RT-rtPCR methods for detecting SARS-CoV2. All participating laboratories were able to detect the viral RNA spiked in buffer and PrimeStore molecular transport medium (MTM). With ILC2, Vet-LIRN extended ILC1 by evaluating analytical sensitivity and specificity of the methods used by participating laboratories to detect 3 SARS-CoV2 variants (B.1; B.1.1.7 [Alpha]; B.1.351 [Beta]) at various copy levels. We analyzed 57 sets of results from 45 laboratories qualitatively and quantitatively according to the principles of ISO 16140-2:2016. More than 95% of analysts detected the SARS-CoV2 RNA in MTM at ≥500 copies for all 3 variants. In addition, for nucleocapsid markers N1 and N2, 81% and 92% of the analysts detected ≤20 copies in the assays, respectively. The analytical specificity of the evaluated methods was >99%. Participating laboratories were able to assess their current method performance, identify possible limitations, and recognize method strengths as part of a continuous learning environment to support the critical need for the reliable diagnosis of COVID-19 in potentially infected animals and humans. 相似文献
4.
Laura Cobelli Marina Collina Agostino Brunelli 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(5):449-455
Control of lettuce downy mildew (Bremia lactucae) with phenylamide fungicides has failed in some intensive lettuce-producing areas in Northern Italy since Spring 1993. Before then, these chemicals and particularly metalaxyl, provided the best disease control. The sensitivity of Bremia lactucae isolates collected in such areas to metalaxyl was evaluated in the laboratory. These strains grew and sporulated profusely on lettuce seedlings treated with 100 and 200 ppm of metalaxyl, whereas sensitive control strains were completely inhibited when treated with fungicide concentrations ranging from 0.5–1 ppm. Thus in Italy occurrence of resistance to phenylamide fungicides in Bremia lactucae has also been demonstrated, as in almost all the countries where these chemicals were previously authorised. Subsequently, a demonstration of occurrence of resistance was made and the virulence pattern of several strains (resistant and sensitive to metalaxyl) was characterised using differential NL series containing the 13 DM resistant genes or R factors. The results suggest the occurrence of a new pathotype in Italy different from all the 16 NL Bremia lactucae races studied. 相似文献
5.
Claire N. Lieske James H. Clark Howard G. Meyer Leigh Boldt Martin D. Green John R. Lowe Walter E. Sultan Peter Blumbergs Matthew A. Priest 《Pesticide biochemistry and physiology》1984,22(3):285-294
The inhibition of eel acetylcholinesterase by the 4-nitrophenyl esters of 2-furyl(methyl)-, methyl(2-thienyl)-, di-2-furyl-, and di-2-thienylphosphinic acid (I, II, III, and IV, respectively) was investigated at pH 6.90 in 0.067 M phosphate buffer (25.0°C) using stopped-flow instrumentation and automated data processing. Our evaluation of the dissociation constant, Kd, the unimolecular bonding rate constant, k2, and the bimolecular reaction constant, ki, are the first reported values for these constants for alkyl/heteroaryl and diheteroaryl esters of phosphinic acids. The largest ki value (19,330 M?1 sec?1) was observed for the reaction of I with the enzyme. The order for the remaining three is II > IV > III. There is no direct relationship between the hydrolysis rates of the esters and their anticholinesterase activities on eel acetylcholinesterase. Likewise, there is no direct relationship between their anticholinesterase activities and the LD50 values in rats. 相似文献
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A total of 356 piglets from one farm were examined for foot and skin lesions every day for the first 10 days after birth, and then on alternate days until they were weaned. Over a period of 24 days 100 per cent of the piglets examined developed sole bruising, and 49.1 per cent developed sole erosions. Sole bruising lasted for an average of 13 days and sole erosions for seven days. At the beginning of the study, a higher proportion of piglets had mild sole bruising; from three to nine days of age piglets had moderate sole bruising but from days 10 to 20 mild bruising was again observed more frequently. Skin lesions were observed on the carpal aspect of the front limbs; 60.9 per cent of the piglets developed skin abrasions, 70.7 per cent developed healed wounds and 90 per cent developed hairless patches. The skin abrasions lasted on average for six days, the healed wounds for five days and the hairless patches for eight days. Piglets which developed sole bruising and/or sole erosions on the first day of life were significantly heavier than those which did not. Continuous observations of the piglets' behaviour during the first six days of life showed that sole bruising increased as the total time spent in the creep area or lying near the sow on the solid floor increased. During the first three days of life skin abrasions increased as the total time spent lying in the creep area increased. There was a positive correlation between the total time piglets spent suckling and the incidence of carpal skin abrasions between four and six days of age. Piglets with sole bruising, sole erosions or carpal skin abrasions spent less time during the day in 'other' activities such as walking, playing or fighting. 相似文献
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