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排序方式: 共有100条查询结果,搜索用时 491 毫秒
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Linu Kuruvilla MB Mohamed Sathik Molly Thomas Lisha.P Luke Sumesh KV 《Journal of Crop Science and Biotechnology》2017,20(5):369-377
Cold stress is one of the major abiotic factors that influence the productivity and geographical distribution of many agriculturally important crops like Hevea brasiliensis. Cultivation of H. brasiliensis in India is being extended to northeastern regions, where low temperature during winter adversely affects its survival, growth, and productivity. Developing cold-tolerant genotypes is a primary requisite to maximize the productivity under such challenging environmental conditions. However, lack of methods for early evaluation of cold tolerance in the newly developed clones and the extensive time required for assessing their tolerance in the field are major constraints for clonal selection. The present study was initiated with an objective to identify and characterize cold stress responsive miRNAs from H. brasiliensis that show stronger association with cold tolerance. Next generation sequencing using Illumina HiSeq method revealed the expression of 21 and 29 conserved miRNA (from clone RRIM 600) families in cold-stressed and control samples, respectively. Forty-two novel miRNAs were identified from this study. Upon differential expression analysis, eight conserved miRNAs were found commonly expressed in both the samples. When expression analyses were performed subsequently with six selected miRNAs in two Hevea clones (viz. RRII 105 and RRIM 600), miR169 showed a strong association with cold tolerance. miRNAs such as miR482 and miR159 also exhibited association with cold tolerance. This study suggests the possibility of employing these miRNAs as markers for cold tolerance after validation in more number of genotypes with varying levels of cold tolerance. 相似文献
3.
van Diemen PM Kreukniet MB Galina L Bumstead N Wallis TS 《Veterinary immunology and immunopathology》2002,88(3-4):183-196
The degree of resistance to Salmonella choleraesuis infection in a reference family purposely bred to map resistance genes was assessed. Aspects of the innate and specific immune system were studied to find a parameter that might predict the resistance of pigs to salmonellosis. The family was bred from commercial full-sister pairs of F1-gilts and four boars. One boar (G398) was identified as breeding susceptible offspring, and one boar (G402) as breeding resistant offspring on the basis of pyrexial responses and numbers of Salmonella in liver and spleen post mortem. The other two boars were classified as 'possible resistant' (Y2008) and 'unknown' (Y6101) respectively. Functional differences in immune cells (neutrophils and lymphocytes) between the offspring of G398 and G402 were detected. The most resistant piglets had a higher number of circulating neutrophils and better polymorphonuclear neutrophils (PMNs) function, but a lower mitogenic response of lymphocytes both pre- and post-infection and a lower antibody response. Between the offspring groups of Y2008 and Y6101 no differences were found in the number of viable Salmonella in liver and spleen at post mortem or in immune cell function, however, the survival rate of these offspring groups was clearly different. Twenty three percent of the Y2008-offspring and 33% of the Y6101-offspring reached the predetermined humane clinical endpoint before the end of the experiment. Our findings suggest a role for several inherited immunological traits, including PMN function and lecithin-induced mitogenic proliferation, which appear to influence resistance to salmonellosis. 相似文献
4.
C.R.E. Halnan MA Vet MB PhD FRCVS C.F.P. Irwin BVSc MACVSc 《Journal of Equine Veterinary Science》1985,5(6):328-329
A Thoroughbred mare, barren during three years at stud, unresponsive to therapy, and not palpably abnormal anatomically was found to have hypoplasia of the uterine tubes after excision at laparotomy. The cause was an unbalanced genotype. The mosaic karyotype, determined on a blood sample, was indispensible in diagnosis and the case extends the potential for cytogenetic prognostic procedures. 相似文献
5.
JONATHAN L. STOWATER DVM MS CARL A. KIRKER-HEAD MA VET MB MRCVS RICHARD M. JAKOWSKI DVM PHD 《Veterinary radiology & ultrasound》1986,27(4):115-117
Osteochondrosis of the lateral femoral condyles was diagnosed radiographically in an 8-month-old, female Arabian horse, which had been presented with a hindlimb lameness. The diagnosis was confirmed by gross and microscopic pathology. The location of the lesions was considered unusual for osteochondrosis in the horse. 相似文献
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Recombinant Bone Morphogenetic Proteins: Novel Substances for Enhancing Bone Healing 总被引:1,自引:0,他引:1
CARL A. KIRKER-HEAD MA Vel MB MRCVS Diplomate ACVS Diplomate ECVS 《Veterinary surgery : VS》1995,24(5):408-419
Bone morphogenetic proteins (BMPs) are differentiative factors whose principal function is to induce transformation of undifferentiated mesenchymal cells into chondroblasts and osteoblasts in a dose-dependent manner. Bone morphogenetic proteins have been isolated postnatally in mammals from bone matrix, periosteal cells, mesenchymal cells of marrow stroma, tooth anlagen, and cells of osteosarcoma and chondrosarcoma. Distribution in additional embryonic tissues implies a broader organogenic function. Bone morphogenetic proteins are the only differentiative factors able to singularly induce de novo bone formation in vitro and in vivo. Recombinant DNA technology allows their production in large and highly purified quantities. The BMPs' osteoinductive ability has been shown with a variety of carriers including collagens and polymers at heterotopic and orthotopic sites in a wide range of species. They are presently being readied for clinical use as alternatives to bone grafts. Other potential applications include use as pulp capping agents, promoters of implant osteointegration and soft tissue reunion with bone, treatments for nonadaptive bone disease, and implants for use with mitotically expanded skeletal stem cell populations. Errors in the genetic coding of BMPs may manifest as clinical disease entities. 相似文献
8.
Malm O Guimarães JRD Castro MB Bastos WR Viana JP Branches FJP Silveira EG Pfeiffer WC 《Water, air, and soil pollution》1997,97(1-2):45-51
Informal economy of gold mining has contaminated some important river basins in Amazon. Follow-up studies on critical compartments showed some areas with high Hg levels in fish as well as in human hair samples. Average Hg in piscivorous fish in the Madeira river itself was 846 ppb (N=284) with a maximum of 3921 ppb. Mercury in fish from non polluted areas in this basin shows high variability, even for single species. A seasonal variation in Hg content was observed, with higher values at the end of the dry season. In the upper Tapajós basin comparable values were found for fish but with a definite decreasing trend downstream. Average value for piscivorous fish in the whole Tapajós basin is 482 ppb (N=122) with a maximum value of 3770 ppb. Hair Hg was higher in fishing villages in the Tapajós (average: 17 ppm; with N=432 and maximum value of 176 ppm) than in the Madeira (average: 9 ppm; N=169; maximum 71 ppm), and data from some areas of the Tapajós suggest a decrease with time. Mercury was much higher in urine of goldshop workers in Santarém (low Tapajós) than in Alta Floresta (high Tapajbs) and show a decreasing trend in both cases, probably related to the significant decline in gold mining activities during the study period (1986–1994). 相似文献
9.
The situation in Thoroughbred breeding in relation to cytogenetics is considered and it is indicated where screening of horses will be useful in practice. The techniques involved are explained and karyotypes are illustrated. The present state of knowledge in horse cytogenetics is reviewed. The types of abnormality are described in relation to phenotype. Future promise is discussed in relation to the human field, concluding that with the refined methods now available a wider rangeof meaningful aberrations will be uncovered. 相似文献
10.
BRADLEY R. JACKMAN DVM MS GARY M. BAXTER VMD MS DIPIOMATE ACVS RICHARD E. DORAN DVM MS Dipiomate ACVS DOUGLAS ALLEN DVM MS Dipiomate ACVS ANDREW H. PARKS MA VET MB Dipiomate ACVS 《Veterinary surgery : VS》1993,22(4):285-288
The medical records of 57 horses that had palmar digital neurectomy performed between 1984 and 1990 were reviewed. Neurectomies were performed either by transection and elec-trocoagulation (47 horses) or by the guillotine technique (10 horses). Middle-aged geldings, Quarter Horses, and Thoroughbreds were significantly over-represented when compared with the hospital population. Horses used as hunter/jumpers also appeared to be over-represented. Complications occurred in 17 (34%) of the 50 horses for which follow-up information was obtained. Recurrence of heel pain was the most common complication (14 horses). Palpable painful neuromas were detected in three horses. One year after neurectomy, 74% of the horses were sound; this decreased to 63% after 2 years. 相似文献