不同稀释液对藏獒精液保存效果的影响

比较了8种稀释液在藏獒精液冷冻保存和低、常温保存(5 ℃,10 ℃,15 ℃,20 ℃)的效果.结果表明:2号和8号稀释液在冷冻保存时效果最好,精子活力和顶体完整率分别达到0.40和49%,与对照组相比差异显著(P<0.05).在藏獒的低温、常温保存试验中,所有稀释液在15 ℃时保存时间最长,综合考虑,7号液保存效果最好;在15℃时精子平均存活时间为103 h,显著高于对照组和其它稀释液(P<0.05).     

新的信息资源环境下图书馆员角色转变的思考

本文分析了信息时代图书馆员角色转变的几个重要因素及特点,揭示了新时代图书馆员应承担的几种重要角色,对信息时代图书馆员的发展进行了探讨.     

对农业推广的信息服务

本文论述了农业信息服务与农业推广的相互关系，分析了图书馆在农业推广的农业信息服务工作中的作用和功能，并提出了相关的一些构想。     

能源甘蔗的开发利用

本文介绍国内外能源甘蔗育种现状，慨述能源甘蔗在能源、糖料、饲料等方面的利用及其副产品的综合开发利用，说明能源甘蔗作为一种新型的生物能源，具有广阔的发展前景。     

国产MJ3210小型木工跑车带锯机锯解正交试验分析

采用正交试验设计法，对国产MJ3210小型木工跑车带锯机的锯解质量进行研究。通过实测马尾松锯材尺寸偏差、表面粗糙度等参数，得到其最佳的进料速度、锯解厚度和锯卡高度的组合方案。结果表明，该设备具有结构简单、出材率高、锯解精度高、质量好、成本低和生产效率高等优点。     

供水工程影响下中国北方地区耕地后备资源开发潜力

灌溉水源是北方地区耕地后备资源能否有效开发的关键。当前,中国正在推动国家水网工程规划建设,为北方地区耕地后备资源开发提供了新的机遇。在以往研究基础上,该研究考虑北方地区已建、在建及规划的供水工程,基于自然适宜、利用高效、发展稳定三方面评价准则,结合三维魔方空间分类方法,开展了雨养情景和供水灌溉情景下耕地后备资源开发潜力分析。结果表明：雨养农业情景下,北方地区勉强适宜（Ⅱ级）、中度（Ⅲ级）和高度（Ⅳ级）适宜的耕地后备资源面积分别为2.9万、1.0万和0.6万km²。灌溉农业情景下,耕地后备资源面积明显增加,勉强适宜（Ⅱ级）、中度（Ⅲ级）和高度（Ⅳ级）适宜的耕地后备资源面积将分别达到5.4万、7.6万和6.5万km²,主要集中于新疆、内蒙古和甘肃3省（自治区）。该研究结果可为中国耕地后备资源开发利用及国家水网工程效益发挥提供参考。     

Selection and amplification of the liver stem cell subset from rat bone marrow cells with a medium containing cholestatic serum in vitro

AIM: To explore the feasibility of direct separat and selective enlargement of the bone marrow-derived liver stem cells (BDLSC) from bone marrow cells with a culture system containing cholestatic serum in vitro. METHODS: Bone marrow cells of rats were cultured with selective media containing 2%, 5%, 7% and 10% cholestatic rat serum, respectively. The BDLSC were then induced to proliferate with the addition of hepatocyte growth factor (HGF) on the firth day. BDLSC were characterized using immunocytochemistry and RT-PCR for lineage markers, glycogen staining and urea synthetic assay for functions 2 weeks later. RESULTS: Bone marrow cells were unble to form colony in the presence of 2% cholestatic serum and apopotosis appeared gradually in 7% or 10% cholestatic serum. The BDLSC survived in the medium containing 5% cholestatic serum while the other types of cells did not. The survival cells proliferated with a high speed during the second week and then formed hepatocyte-like colony-forming units (H-CFU). Cells in the H-CFU expressed the characteristic proteins of fetal hepatocytes. Furthermore, they had glycogen storage and urea synthesis functions, two of the critical features of hepatocytes. CONCLUSION: The selective micro-environment effectively selected BDLSC from the bone marrow cell, and will be a new way to provide an abundant source of donor hepatocytes for clinical cell therapy.     

Effects of component of some Chinese herbs on proliferation of human umbilical vein endothelial cells in vitro

AIM: To observe the effects of some component of Chinese herbs for external use on proliferation of human umbilical vein endothelial cells (HUVEC) and investigate the mechanism of promoting tissue repair. METHODS: The method of MTT was used to examine the effects of Rg1, Rh1, perlolyrine, cinnamyl aldehyde, muscone, astragaluspolysaccharin (APS), velver antler polypeptide (VAP) and soluble extract of boswellia carterii birdw (BCB) on proliferation of HUVEC. RESULTS: APS did not promote proliferation of HUVEC at 9.75 mg/L-2.5 g/L; Rh1 promoted proliferation of HUVEC at 1.94 mg/L-0.5 g/L (P<0.05 or P＜0.01), and Rg1 inhibited proliferation of HUVEC at 31 mg/L (P<0.05); VAP promoted proliferation of HUVEC at 1 mg/L-0.5 g/L with optimal dose of 10 mg/L (P<0.01), Cinnamyl aldehyde promoted proliferation of HUVEC at 2 g/L(P＜0. 05); Muscone and soluble extract of BCB inhibited proliferation of HUVEC at 1 g/L, 0.5-2.5 kg/L(P＜0. 01), respectively; Perlolyrine inhibited proliferation of HUVEC at 0.125 g/L-0.5 g/L(P＜0. 01). CONCLUSION: The external herbs for supplementing Qi and warming Yang can promote HUVEC proliferation and improve angiogenesis during tissue repair. The external herbs for promoting blood circulation and accelerating capillary movement may have influence upon other stages of tissue repair.     

The molecular mechanism of inhibition of murine Lewis lung carcinoma metastasis by weimaining in vivo

AIM: To investigate the anti-metastasis effect of weimaining, extracted from fragopyrum cymosum meissn, a Chinese medicine, on murine Lewis lung carcinoma (3LL). METHODS: The anti-metastasis effect of weimaining in vivo was detected in the grafting lung metastasis model of murine Lewis lung carcinoma. The effects of the drug on the expression of CD34 and E-cadherin were investigated by immunohistochemical staining and RT-PCR. RESULTS: Weimaining effectively inhibited the lung metastasis of 3LL at a concentration of 250 mg·kg^-1·d^-1, significantly suppressed the expression of CD34 and increased the expression levels of E-cadherin protein and mRNA in 3LL cells. CONCLUSIONS: Weimaining inhibits the metastasis of murine Lewis lung carcinoma (3LL) in vivo via increasing the expression of E-cadherin and decreasing microvessel density of tumor tissue.