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Reduction of N2O in moist soil was inhibited completely by 10?2 atm C2H2 and partially by 10?5 atm C2H2. The effect of C2H4 was 104 times less than that of C2H2. Denitrification of NO?3 occurred in anaerobically or aerobically incubated waterlogged soil and in anaerobic but not in aerobic moist soil. In the absence of C2H2 there was transient accumulation of N2O. In the presence of C2H2 there was stoichiometric conversion of NO?3 to N2O. Some kinetics of the reduction of N2O and of NO?3 to N2O are presented. Denitrification of 1 μg added NO?3-N.g? could be measured within 1 h. Stoichiometries of production of N2O from NO?2 and NO?3, respectively, and production of CO2 attributable to denitrification were consistent with reported energy yields. Reduction of C2H2 to C2H4 occurred immediately following complete denitrification of added NO?3. The incubation of soil in the presence and in the absence of C2H2 thus permits assay of both denitrification and N2 fixation and provides information on the mole fraction of N2O in the products of denitrification. 相似文献
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Sandy loam soil was amended with different concentrations of glucose and was incubated at different pO2 levels. Under many conditions of incubation time and treatment, N2 ase activity as determined by 1-h aerobic C2H2 reduction assay (flushed with Ar:O2, 4:1 before assay) was significantly less than that determined by means of ambient assay (carried out at the pO2 of incubation without flushing with Ar:O2, 4:1 before assay). The difference between the N2ase activity in aerobic assay and that in ambient assay increased with decreasing glucose and O2 concentrations imposed during incubation. The inhition in aerobic assays was analogous to O2-induced shut-off of N2ase and amounted to 75 per cent inhibition after incubation at 0.06 atm pO2 of samples amended with 0.75% glucose (w/w). Similar O2 inhibition was observed after amendment with mannitol and with lactate. Times of incubation were chosen such that development of anaerobic N2ase activity was either absent or too low to account for the observed effects of O2 during assay. It was shown that 0.05 atm pC2H2 was adequate for routine 1-h assays of the soil system employed. Individual soil samples could be subjected to repeated 1-h assays (with removal of C2H2 and C2H4 by evacuation after each assay) thus avoiding side-effects of long exposure to C2H2. 相似文献
4.
Mechanisms associated with bendiocarb resistance were examined in two strains of western flower thrips, Frankliniella occidentalis (Pergande), that differed in their susceptibility to this carbamate by 13.6-fold. No appreciable differences between the two strains in [14C] bendiocarb penetration and excretion were detected; however, bendiocarb was metabolised substantially faster by the resistant KCM thrips than by the more susceptible UMC thrips. No appreciable difference between the two strains was found in the sensitivity of acetylcholinesterase activity to inhibition by bendiocarb. It was concluded that bendiocarb resistance in KCM western flower thrips was due to enhanced metabolism that probably was mainly oxidative in nature. 相似文献
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Mannheimia haemolytica is an important etiological agent of pneumonia in domestic sheep (DS, Ovis aries). Leukotoxin (Lkt) produced by this organism is the principal virulence factor responsible for the acute inflammation and lung injury characteristic of M. haemolytica caused disease. Previously, we have shown that the leukocyte-specific integrins, beta(2) integrins, serve as the receptor for Lkt. Although it is certain that CD18, the beta subunit of beta(2) integrins, mediates Lkt-induced cytolysis of leukocytes, it is not clear whether CD18 of all three beta(2) integrins, LFA-1, Mac-1 and CR4, mediates Lkt-induced cytolysis of DS leukocytes. Since polymorphonuclear leukocytes, which express all three beta(2) integrins, are the leukocyte subset that is most susceptible to Lkt, we hypothesized that all three beta(2) integrins serve as the receptor for Lkt. The objective of this study was to determine whether DS LFA-1 serves as a receptor for M. haemolytica Lkt. We cloned the cDNA for DS CD11a, the alpha subunit of LFA-1, and co-transfected it along with the previously cloned cDNA for DS CD18, into a Lkt-non-suceptible cell line. Transfectants stably expressing DS LFA-1 were bound by Lkt. More importantly, Lkt lysed the DS LFA-1 transfectants in a concentration-dependent manner. Pre-incubation of Lkt with a Lkt-neutralizing monoclonal antibody (MAb), or pre-incubation of transfectants with MAbs specific for DS CD11a or CD18, inhibited Lkt-induced cytolysis of the transfectants. Exposure of LFA-1 transfectants to low concentrations of Lkt resulted in elevation of intracellular [Ca(2+)](i). Taken together, these results indicate that DS LFA-1 serves as a receptor for M. haemolytica Lkt. 相似文献
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Toby G Knowles Steve N Brown Paul D Warriss Jeff Lines Ambrose Tinarwo Marta Sendon 《Aquaculture Research》2008,39(16):1731-1738
Food quality aspects of farmed turbot (Psetta maxima) were compared following two methods of slaughter: the current commercial method, by immersion in an ice slurry, which is then dewatered after approximately 20 min, or by first humanely, electrically stunning the fish using a prototype commercial stunner, before immersion in an ice slurry, which is dewatered after 20 min. Quality was assessed for up to 10 days of storage on ice following slaughter. No differences were found between the slaughter methods in terms of an overall carcass quality: overall appearance, haemorrhage, damage, burst gall bladder, staining of the body cavity by leakage from the gut or damage to the spine. No detectable difference was found between the treatments using the industry standard freshness scoring system, the Quality Index Method. Both groups of fish were classified as ‘Fresh’ after 10 days of storage on ice. Using objective measurements of colour, no differences between fish from either treatment were found in fillet colour. Changes in flesh pH were similar in electrically stunned and traditionally killed fish with a mean pH (±SE) at 2 h post‐mortem of 6.80±0.027 declining to 6.44±0.032 at 24 h post‐mortem. Humane electrical stunning of turbot at slaughter neither detectably improved nor decreased product quality as measured between 1 and 10 days of storage on ice. 相似文献
10.
黄土耕作坡面溅蚀过程中微地形响应特征 总被引:4,自引:0,他引:4
[目的]探究60mm/h雨强下不同耕作坡面在溅蚀阶段的微地形变化特征,为黄土坡地水土保持耕作方式布设提供科学依据。[方法]在室内人工模拟降雨试验的基础上,采用激光扫描量测方法分别获取溅蚀前后4种不同耕作方式(人工锄耕、人工掏挖、等高耕作、耙磨整平)黄土坡面微地形相对高程,并对能反映微地形变化的高精度M-ΔDEM进行了分析。[结果]不同耕作措施下坡面微地形均具有较弱的空间变异,其空间分异主要受耕作措施的影响;降雨对微地形具有一定的夷平效应,且不同耕作坡面在1~1.3m尺度范围内均表现出侵蚀平衡现象;不同耕作微地形对溅蚀均具有阻滞作用,其中人工掏挖和等高耕作具有较强的水土保持作用。[结论]微地形水系分维能较好地反映复杂的微地形特征,而坡面耕作方式是溅蚀过程中微地形时空变异分布状况的控制性因子。 相似文献