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排序方式: 共有388条查询结果,搜索用时 15 毫秒
1.
A questionnaire study examining Japanese consumers’ requirements for beef quality was carried out in April 2002 in Tsukuba, Ibaraki, Japan. Answers from 532 participants were used for the statistical analysis. Twenty questions about sensory properties, appearance, safety and freshness, production area, price, and other properties were prepared. The answers were applied to principal component analysis. Five principal components were obtained and defined, and the principle component scores were used to classify participants. The panels were classified into four groups profiled as ‘specific quality orientated’, ‘Wagyu (Japanese beef) orientated’, ‘safety and freshness orientated’ and ‘overall high quality orientated’. In addition, gender and age characteristics were different among the groups. Sensory properties did not affect the classification of respondents. 相似文献
2.
Keisuke SASAKI Tomotaro YOSHIDA Kengo KOGA Takashi HARAGUCHI Kazunori OHASHI Yosuke AOYAGI 《Animal Science Journal》2004,75(6):557-562
The effect of insulin on the ascorbate recycling system in the chicken liver was examined. First, insulin was injected subcutaneously into the chicken, and liver glutathione‐dependent dehydroascorbate reductase (GSH‐DHAR) activity was determined. Insulin increased liver GSH‐DHAR activity, but did not affect plasma and liver ascorbate concentration. Dehydroascorbate increased plasma and liver ascorbate levels, and liver GSH‐DHAR activity. However, distinct changes in plasma insulin level were not observed by dehydroascorbate injection. In addition, reduction of external dehydroascorbate in cultured chicken hepatocytes could not be observed in an insulin‐deprived culture, although the cells reduced external dehydroascorbate in a serum‐free culture with insulin. We concluded that insulin affects the ascorbate recycling system as an essential factor in the chicken liver. 相似文献
3.
Yosuke SASAKI Satoshi SEKIGUCHI Ryoko UEMURA Masuo SUEYOSHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):333-335
This study compared the reproductive and growth performances of pigs before and after depopulation and
restocking after a foot-and-mouth disease outbreak in Japan. Data for the time period before and after
depopulation and restocking were obtained from three farrow-to-finish farms. As a result of depopulation and
restocking, hygiene levels were improved, and common infectious diseases, such as porcine reproductive and
respiratory syndrome and Aujeszky’s disease, remained undetected on the farms. Compared with before
depopulation, reproductive and growth performances improved after depopulation; the number of total pigs born
was higher, the postweaning mortality rate was lower, and the age at slaughter was lower
(P<0.05). In summary, depopulation and restocking improved the reproductive and growth
performances of pigs. 相似文献
4.
Kazuki HARADA Atsushi SASAKI Takae SHIMIZU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(1):83-89
The study objective was to determine the effect of oral orbifloxacin (ORB) on
antimicrobial susceptibility and composition of fecal coliforms in cats. Nine cats were
randomized to two groups administered a daily oral dose of 2.5 and 5.0 mg ORB/kg for 7
days and a control group (three cats per group). Coliforms were isolated from stool
samples and were tested for susceptibilities to ORB and 5 other drugs. ORB concentration
in feces was measured using high-performance liquid chromatography (HPLC). The coliforms
were undetectable after 2 days of ORB administration, and their number increased in most
cats after termination of the administration. Furthermore, only isolates of
Escherichia coli were detected in all cats before administration, and
those of Citrobacter freundii were detected after termination of the
administration. E. coli isolates exhibited high ORB susceptibility
[Minimum inhibitory concentration (MIC), ≤0.125 µg/ml]
or relatively low susceptibility (MIC, 1−2 µg/ml) with a
single gyrA mutation. C. freundii isolates largely
exhibited intermediate ORB susceptibility (MIC, 4
µg/ml), in addition to resistance to ampicillin and
cefazolin, and harbored qnrB, but not a gyrA mutation.
HPLC revealed that the peaks of mean concentration were 61.3 and 141.0
µg/g in groups receiving 2.5 and 5.0 mg/kg, respectively. Our findings
suggest that oral ORB may alter the total counts and composition of fecal coliform, but is
unlikely to yield highly fluoroquinolone-resistant mutants of E. coli and
C. freundii in cats, possibly because of the high drug concentration in
feces. 相似文献
5.
Yukino TAMAMURA-ANDOH Nobuyuki TANAKA Keisuke SATO Yoshino MIZUNO Nobuo ARAI Ayako WATANABE-YANAI Masato AKIBA Masahiro KUSUMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(5):754
We examined the antimicrobial susceptibility of 848 Escherichia coli isolates from 237 feces samples of wild sika deer (Cervus nippon) captured between 2016 and 2019 in 39 of the 47 prefectures of Japan. Five of the 237 wild sika deer (2.1%) carried E. coli with resistance to at least one antimicrobial, and all the resistant isolates showed resistance to tetracycline. The resistant isolates contained antimicrobial resistance genes that were similar to those in E. coli derived from humans and farm animals. Although wild sika deer are not currently likely to be a source for the transmission of antimicrobial resistance in Japan, they can potentially mediate antimicrobial resistance spread by coming into contact with humans, animals, and their surroundings. 相似文献
6.
Ahram KIM Naoki SASAKI Inhyung LEE Jong-pil SEO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(4):643
The purpose of this study was to assess the cardiorespiratory and behavioral responses to the combination of medetomidine and tramadol (M-T) or butorphanol (M-B) in standing laparoscopic ovariectomy in horses. One ovary was removed under M-T and the contralateral ovary was removed under M-B with at least 4 weeks between operations at random. Horses were sedated using intravenous medetomidine (5 µg/kg) followed by tramadol (1 mg/kg) or butorphanol (10 µg/kg) after 5 min. Sedation was maintained through the repeated injection of medetomidine (1 µg/kg) and tramadol (0.4 mg/kg) or medetomidine (1 µg/kg) and butorphanol (4 µg/kg) every 15 min. Cardiorespiratory function and behavioral responses, including, sedation, ataxia, and analgesia, were assessed during the surgery. There were no significant differences in cardiorespiratory values and sedation and analgesia scores between M-T and M-B. Ataxia scores were significantly lower in M-T than in M-B. This result suggests that M-T could maintain smooth and stable standing surgery with minimal cardiorespiratory changes in horses. 相似文献
7.
Expression dynamics of bovine MX genes in the endometrium and placenta during
early to mid pregnancy
Takahiro SHIROZU Keisuke SASAKI Manabu KAWAHARA Yojiro YANAGAWA Masashi NAGANO Nobuhiko YAMAUCHI Masashi TAKAHASHI 《The Journal of reproduction and development》2016,62(1):29-35
MX belongs to a family of type I interferon (IFN)-stimulated genes, and the MX protein has
antiviral activity. MX has at least two isoforms, known as MX1 and
MX2, in mammals. Moreover, bovine MX1 has been found to have alternative
splice variants—namely, MX1-a and MX1B. In ruminants, IFN-τ—a type I IFN—is
temporarily produced from the conceptus before implantation and induces MX expression in the
endometrium. However, the expression dynamics of MX after implantation are not clear. In the
present study, we investigated the expression of MX1-a, MX1B and
MX2 in the endometrium and placenta before and after implantation along with the expression
of IFN-α, type I receptors (IFNAR1 and IFNAR2) and
interferon regulatory factors (IRF3 and IRF9). Pregnant uterine samples were
divided into five groups according to pregnancy days 14–18, 25–40, 50–70, 80–100, and 130–150. Tissue samples
were collected from the intercaruncular endometrium (IC), caruncular endometrium (C) and fetal placenta (P).
Although all the MX expressions were significantly higher in the IC and C at days 14–18,
presumably caused by embryo-secreted IFN-τ stimulation, their expressions were also detectable in the IC, C
and P after implantation. Furthermore, IFN-α expression was significantly higher in the IC.
RT-PCR indicated IFNAR1, IFNAR2, IRF3 and
IRF9 mRNA in all the tissues during pregnancy. These results suggest that all the
MX genes are affected by the type I IFN pathway during pregnancy and are involved in an
immune response to protect the mother and fetus. 相似文献
8.
Md. Shah Alam Sarker Shuichi Satoh Keisuke Kamata Yutaka Haga Yoshihiro Yamamoto 《Aquaculture Research》2012,43(4):538-545
A feeding experiment was conducted to investigate the effect of organic acids and/or lipid supplementation on growth, utilization and environmental loading of nitrogen (N) and phosphorus (P) in juvenile yellowtail fed fishmeal (FM) and plant protein (PP) diets. Six diets as FM (FM‐based), FM+P (FM with inorganic P), FM+L (FM with lipid), PP+CA (PP with citric acid), PP+L+CA (PP with lipid and citric acid) and PP+L+FA (PP with formic acid) were formulated. Yellowtails were fed each of the diets in duplicate groups; once a day, 6 days a week to near satiation at water temperature 19.0–25.0 °C for 16 weeks. Fishmeal with inorganic P gave the best growth while PP+L+FA the lowest. However, growth increased in PP+CA and PP+L+CA. Addition of lipid significantly increased N and P retention resulting in significant reduction in N and P excretion. Citric acid and FA supplementation to PP diets also increased retention of P; hence, its excretion was lowered. Thus, CA, FA and lipid in juvenile yellowtail diets can help to partially replace FM with PP sources and reduce inorganic P use to minimize environmental loading from aquafeeds. 相似文献
9.
Loida O. Casalme Keisuke Katayama Yoshiki Hayakawa Kensuke Nakamura Arisa Yamauchi Yasuyuki Nogata Erina Yoshimura Fuyuhiko Matsuda Taiki Umezawa 《Marine drugs》2022,20(2)
Some derivatives of dolastatin 16, a depsipeptide natural product first obtained from the sea hare Dolabella auricularia, were synthesized through second-generation synthesis of two unusual amino acids, dolaphenvaline and dolamethylleuine. The second-generation synthesis enabled derivatizations such as functionalization of the aromatic ring in dolaphenvaline. The derivatives of fragments and whole structures were evaluated for antifouling activity against the cypris larvae of Amphibalanus amphitrite. Small fragments inhibited the settlement of the cypris larvae at potent to moderate concentrations (EC50 = 0.60-4.62 μg/mL), although dolastatin 16 with a substituent on the aromatic ring (24) was much less potent than dolastatin 16. 相似文献