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排序方式: 共有141条查询结果,搜索用时 23 毫秒
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Kanae SHIOKAWA Chandika D. GAMAGE Nobuo KOIZUMI Yoshihiro SAKODA Kenta SHIMIZU Yoshimi TSUDA Kumiko YOSHIMATSU Jiro ARIKAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):221-230
The applicability of the recombinant LipL32 for serodiagnosis of leptospiral infection in field rodents was
assessed in this study. An immunodominant region of LipL32 was determined by monoclonal antibodies, and then,
truncated LipL32 (tLipL32) was designed to contain the region (87–188th amino acid). The tLipL32 was compared
between two recombinant expression hosts Escherichia coli and Pichia
pastoris in ELISA. With field rat sera, tLipL32 expressed by P. pastoris
(tLipL32p) had high antigenicity without background reactions, while tLipL32 expressed by E.
coli (tLipL32e) showed high background reactions, which were reduced by pre-adsorption of sera with
E. coli. To evaluate tLipL32-ELISA, field rat sera were tentatively divided into a
Leptospira infection positive (12 sera) and a negative group (12 sera) based on the results
from flaB gene PCR of kidney samples and WB with whole Leptospira cell.
Consequently, the sensitivity of tLipL32p-ELISA for field rat sera was 83% . A similar result was obtained
from tLipL32e-ELISA with adsorbed sera, (92%). However, sensitivity of tLipL32e-ELISA using sera without an
adsorption treatment was 50%. Regardless of the expression host, tLipL32-ELISA had 100% specificity and
sensitivity in experimentally infected laboratory rats. These results suggest that recombinant LipL32
expressed by P. pastoris is more applicable for serodiagnosis in field rats due to a lack of
background reaction. 相似文献
3.
Kazutaka Kato 《Journal of Forest Research》1997,2(2):75-78
To detect developmental differences inEpinotia granitalis in two host tree speciesCryptomeria japonica andChamaecyparis obtusa, the fourth or fifth instar larvae were collected daily from the two species located on the grounds of the National Forest
Tree Breeding Center, from April 19 to May 9 in 1994 and April 18 to May 10 in 1995. Larvae ofE. granitalis collected fromC. obtusa had heavier body weight, developed to larger adults than those collected fromC. japonica, and mortality from the fourth or fifth instar larval stage to adults onC. obtusa was lower than that onC. japonica. All these facts suggest that larval performance onC. obtusa was higher than that onC. japonica. 相似文献
4.
Nobukazu SAISHU Kazutaka MORIMOTO Hiroshi YAMASATO Hiroichi OZAKI Toshiyuki MURASE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1037-1042
Thirty-eight Aerococcus viridans isolates were obtained from milk from
478 cows with clinical mastitis in a farm during the periods between November 2011 and
February 2012, and between December 2012 and March 2013. Additional isolates were obtained
from processed manure (a mixture of composted manure, straw and hydrated lime) and bedding
materials. The processed manure was later used to cover the floor of the stalls in barns
as bedding materials. The temperatures recorded in the composted and processed manure were
not as high as those generally observed during satisfactory composting. To reveal the
association of A. viridans in manure-related products with intramammary
infection in cows, isolates were characterized by their DNA fragment patterns as
determined by pulsed-field gel electrophoresis (PFGE) and antimicrobial susceptibility
testing. Isolates obtained from milk, processed manure and bedding materials had identical
DNA fragment patterns. Antimicrobial susceptibilities were determined for 29 isolates from
milk, processed manure and bedding materials. Of these, 26 (89.7%) were resistant to
clindamycin, whereas virtually all the isolates were susceptible to 12 other
antimicrobials including cefalosporins that have been used to treat bovine mastitis in
Japan. In vitro, three A. viridans isolates from milk
and an isolate from processed manure survived for 3 hr in Good’s buffer (pH 9) at high
temperature (50°C). The results suggest that the processed manure and bedding materials in
this farm were possible sources of A. viridans that caused infection in
the cows with mastitis. 相似文献
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ABSTRACT: The attacking potential of the scavenging amphipod Scopelocheirus onagawae on artificially injured hatchery-raised Japanese flounder Paralichthys olivaceus juveniles was investigated in relation to the degree of injury on the fish. All injured flounder juveniles were attacked by amphipods regardless of the degree of injury, while non-injured juveniles were not attacked. The attack by amphipods on the juveniles generally depended on the amount of glycine, a main feeding stimulant for the amphipod, released from the injury opening. The swimming ability of flounder juveniles was important to avoid the attack of amphipods. Furthermore, an area of injury allowing the amphipods to cling to the fish affects to the vulnerability of juveniles against the predation of amphipods. This study suggests that scavenging amphipods are potentially involved in the rapid reduction of the number of hatchery-raised juveniles. 相似文献
8.
Donnelly SE Birtcher RC Allen CW Morrison I Furuya K Song M Mitsuishi K Dahmen U 《Science (New York, N.Y.)》2002,296(5567):507-510
High-resolution transmission electron microscopy images of room-temperature fluid xenon in small faceted cavities in aluminum reveal the presence of three well-defined layers within the fluid at each facet. Such interfacial layering of simple liquids has been theoretically predicted, but observational evidence has been ambiguous. Molecular dynamics simulations indicate that the density variation induced by the layering will cause xenon, confined to an approximately cubic cavity of volume approximately 8 cubic nanometers, to condense into the body-centered cubic phase, differing from the face-centered cubic phase of both bulk solid xenon and solid xenon confined in somewhat larger (>/=20 cubic nanometer) tetradecahedral cavities in face-centered cubic metals. Layering at the liquid-solid interface plays an important role in determining physical properties as diverse as the rheological behavior of two-dimensionally confined liquids and the dynamics of crystal growth. 相似文献
9.
Lee KJ Sasaki M Miyauchi A Kishimoto M Shimizu J Iwasaki T Miyake Y Yamada K 《Journal of zoo and wildlife medicine》2011,42(1):128-130
This report describes the use of computed tomography (CT) in a nondomestic species. Postmortem CT was performed on a red kangaroo (Macropus rufus) and a diagnosis of oral osteomyelitis was made. CT examination revealed bony remodeling of the right mandible, an intraosseous lesion of the right temporal bone, muscle necrosis around the right mandible, and the absence of the right, first, upper molar tooth. Cardiac and intrahepatic gas and a distended intestine due to postmortem gas accumulation were also seen. All the lesions identified with CT were also identified by conventional necropsy, except the cardiac and intrahepatic gases. Virtopsy may be a useful procedure for the noninvasive identification of cause of death and as a guide for necropsy in animals. 相似文献
10.
Yamakawa K Aoki T Matsui M Yamada K Ishii M 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(5):641-644
To establish a method to predict postpartum diseases using prepartum back fat thickness (BFT), the peripartum BFTs of 54 healthy multiparous cows before calving, which were diagnosed with postpartum displacement of the abomasum (DA), clinical ketosis or subclinical ketosis were compared with those of healthy cows from 8 weeks before the expected calving date to 8 weeks after calving. The peripartum BFTs of the cows with DA or clinical ketosis were significantly higher than those of healthy cows. The peripartum BFTs of the cows with subclinical ketosis were not significantly higher than those of the healthy cows. 相似文献