毛竹林大小年改“均年”的技术措施研究

毛竹林大小年现象分明,每二年约有六个月的竹叶发黄、脱落、换新小叶时间,这期间竹林光能利用效率不高,严重影响次年的产量。通过小年留笋养竹、大年疏笋、控制新竹数量,并采取切断部分立竹的支鞭,利用竹子生长顶端优势,多发叉鞭,造成大小年立竹的多鞭系统竹林结构,同时结合松土、施肥,改善土壤理化性质,提高竹林光能利用率,使每年都有出笋成竹,其产量可比大小年分明的竹林增产20～30％。     

Anti‐tumour activity of oncolytic reovirus against canine histiocytic sarcoma cells

Canine histiocytic sarcoma is an aggressive, fatal neoplastic disease with a poor prognosis. Lomustine is generally accepted as the first‐line systemic therapy, although this compound does not provide complete regression. Therefore, research into a novel approach against canine histiocytic sarcoma is needed. However, anti‐tumour effects of oncolytic therapy using reovirus against histiocytic sarcoma are unknown. Here, we showed that reovirus has oncolytic activity in canine histiocytic sarcoma cell lines in vitro and in vivo. We found that reovirus can replicate and induce caspase‐dependent apoptosis in canine histiocytic sarcoma cell lines. A single intra‐tumoural injection of reovirus completely suppressed the growth of subcutaneously grafted tumours in NOD/SCID mice. Additionally, we demonstrated that susceptibility to reovirus‐induced cell death was attributable to the extent of expression of type I interferons induced by reovirus infection in vitro. In conclusion, oncolytic reovirus appears to be an effective treatment option for histiocytic sarcoma, and therefore warrants further investigation in early clinical trials.