排序方式: 共有26条查询结果,搜索用时 250 毫秒
1.
Kasumi Sudo Mariko Ochiai Naoyuki Aihara Noriyuki Horiuchi Atsushi Yamamoto Sachiko Matsumoto Koji Oishi 《Journal of toxicologic pathology》2021,34(2):137
Batch safety tests (BSTs) of veterinary vaccines are conducted using small laboratory animals to assure the safety of vaccines according to several criteria, including clinical signs and change in body weight. Although the latter is used as an evaluation index in BSTs, there have been no reports on the internal changes that affect body weight during the test period. Therefore, we analyzed BST via pathological examination of the tested animals. Here, BSTs were performed for 176 batches using mice and 126 batches using of guinea pigs. Most of the gross findings could be classified into four lesion types (nodules, adhesions, ascites, no apparent signs), with only one vaccine inducing lesions that could not be classified into any of these four types. Histopathological examination revealed that the reactions caused by BST were pyogenic and/or granulomatous inflammation. Nodular or adhesive lesions comprised more severe pyogenic granulomatous inflammation than ascites or cases with no apparent gross lesions. These nodular or adhesive lesions were more frequently induced by vaccines that contained an adjuvant than by vaccines that did not contain an adjuvant. The cases with “exceptional” gross findings histologically presented severe necrosis of the hematopoietic system. Additional testing showed that these “exceptional” lesions were induced when a specific type of light liquid paraffin was injected along with other vaccine additives. Our results show that body weight loss and/or lesions during BST were induced by proinflammatory properties of the tested vaccines and that BST is a sensitive method for detecting unexpected effects of vaccine components. 相似文献
2.
Ryota MATSUYAMA Naotoshi KUNINAGA Tomoya MORIMOTO Tetsuya SHIBANO Akiko SUDO Kazunari SUDO Makoto ASANO Masatsugu SUZUKI Tetsuo ASAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1179-1181
Plesiomonas shigelloides is a causal agent of gastroenteritis, sepsis
and meningitis in humans. We examined the prevalence of P. shigelloides
among great cormorants (Phalacrocorax carbo hanedae) in Japan and the
antimicrobial susceptibility of isolates. P. shigelloides was isolated
from 33 (47.8%) of 69 fecal samples from great cormorants in 2014. All 33 isolates were
subjected to antimicrobial susceptibility testing using broth microdilution methods, which
showed resistance to ampicillin (31 isolates, 93.9%), tetracycline (two isolates, 6.1%)
and trimethoprim (one isolate, 3.0%). The high prevalence of P.
shigelloides in the great cormorants implicates the possible microbiological
risk to public health. 相似文献
3.
In the present study, the effect of passage of nuclear donor cells on the in vitro development of nuclear transfer (NT) embryos was investigated using colostrum‐derived mammary gland epithelial (MGE) cells at different passages (3–30 passages) to find reliable passages for the efficiency of cloning. Development of NT embryos to the blastocyst stage was affected by the number of passages of MGE cells (P < 0.05). Nuclear transfer embryos reconstructed with MGE cells at 3–7 passages showed a significantly higher blastocyst development (31.3–48.5%) than those with the cells at 10–30 passages (2.5–12.5%, P < 0.05). No difference in the proportion of the MGE cells with normal diploid was observed among passage of 3, 15 and 30 (P > 0.05). The use of MGE cells at early passages for nuclear donor cells may be advantageous for the production of NT embryos. 相似文献
4.
5.
Kazumasa HONDA Ayako SUDO Kentaro IKEDA 《The Journal of reproduction and development》2013,59(6):569-574
The present experiments were undertaken to examine whether oxytocin cells in the
supraoptic nucleus receive synaptic inputs from the contralateral supraoptic nucleus
or paraventricular nucleus. Using urethane-anesthetized lactating rats, extracellular
action potentials were recorded from single oxytocin or vasopressin cells in the
supraoptic nucleus. Electrical stimulation was applied to the contralateral
supraoptic nucleus or paraventricular nucleus, and responses of oxytocin or
vasopressin cells were analyzed by peri-stimulus time histogram or by change in
firing rate of oxytocin or vasopressin cells. Electrical stimulation of the
contralateral supraoptic nucleus or paraventricular nucleus did not cause antidromic
excitation in oxytocin or vasopressin cells but caused orthodromic responses.
Although analysis by peri-stimulus time histogram showed that electrical stimulation
of the contralateral supraoptic nucleus or paraventricular nucleus caused orthodromic
excitation in both oxytocin and vasopressin cells, the proportion of excited oxytocin
cells was greater than that of vasopressin cells. Train stimulation applied to the
contralateral supraoptic nucleus or paraventricular nucleus at 10 Hz increased firing
rates of oxytocin cells and decreased those of vasopressin cells. The results of the
present experiments suggest that oxytocin cells in the supraoptic nucleus receive
mainly excitatory synaptic inputs from the contralateral supraoptic nucleus and
paraventricular nucleus. Receipt these synaptic inputs to oxytocin cells may
contribute to the synchronized activation of oxytocin cells during the milk ejection
reflex. 相似文献
6.
Hibiya K Kasumi Y Sugawara I Fujita J 《Comparative immunology, microbiology and infectious diseases》2008,31(4):347-366
The aim of this study was to classify the histopathological features of pigs infected with Mycobacterium avium complex (MAC). We used slaughtered pig organs systemically infected with MAC. The results showed granulomatous lesions which were observed predominantly in the digestive organs and regional lymph nodes rather than respiratory organs. The histological picture showed a wide range of granulomatous stages from exudative to fibrotic reactions to the MAC infection. Eosinophils and giant cells were characteristically observed in the exudative reactions. The histopathological type in primary focus tended to be maintained in the respective organs. Most strains with the same genotype showed pathogenicity for guinea pigs irrespective of the type of granuloma. Although these findings suggest that different stages of a granulomatous lesion originating from the same causative agent might influence histological patterns, other possibilities such as the hereditary background of the host, or the effects of viral infections should be considered. 相似文献
7.
8.
Hiromichi MITAKE Naoto ITO Kota OKADERA Kazuma OKADA Kento NAKAGAWA Tomomi TANAKA Kiyohito KATSURAGI Kasumi KASAHARA Toshihide NIHONGI Hiroshi TSUNEMITSU Makoto SUGIYAMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(2):221-224
A total of 568 normal feces from calves on a beef farm in Fukui Prefecture, Japan, in 2011–2012 were examined by RT-semi-nested PCR for rotavirus A (RVA) VP4 genes. Through partial sequencing and BLAST analyses of 84 VP4-positive specimens, we identified an avian-like RVA strain, N2342, which shares highest nucleotide identity (80.0%) with known avian-like bovine strain 993/83, in one specimen. Phylogenetic analysis also revealed a close genetic relationship between N2342 and avian RVAs, suggesting bird-to-cattle transmission. We observed frequent contact of wild birds with calves in the farm, suggesting that these birds were the source of the virus. 相似文献
9.
Kasumi SUDO Manabu YAMADA Mariko OCHIAI Shoko IWAMOTO Hajime SAKAKI Chiho KUSHIDA Maiko YAMASHITA Atsushi YAMAMOTO Takao NAGASAKA Mikio OIDEMIZU Akito SAITO Kinya YAMAMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(6):990
We evaluated the role of classical swine fever virus (CSFV) in the formation of button ulcers in the mucosa of the gastrointestinal tract. Histopathological and immunohistochemical analyses of pigs experimentally infected with a subgenotype 2.1 isolate of CSFV, which was isolated in Japan in 2019, revealed follicular necrosis in the submucosal mucosa-associated lymphoid tissue and herniation of crypts as factors that contribute to the development of button ulcers during CSFV infection. These findings indicate that CSFV induces follicular necrosis and is one of the causative agents of button ulcers in pigs. 相似文献
10.
Kazuhiro UMEYAMA Kasumi HONDA Hitomi MATSUNARI Kazuaki NAKANO Tatsuro HIDAKA Keito SEKIGUCHI Hironori MOCHIZUKI Yasuhiro TAKEUCHI Tsukasa FUJIWARA Masahito WATANABE Masaki NAGAYA Hiroshi NAGASHIMA 《The Journal of reproduction and development》2013,59(6):599-603
Somatic cell nuclear transfer (SCNT) is a useful technique for creating pig strains
that model human diseases. However, production of numerous cloned disease model pigs
by SCNT for large-scale experiments is impractical due to its complexity and
inefficiency. In the present study, we aimed to establish an efficient procedure for
proliferating the diabetes model pig carrying the mutant human hepatocyte nuclear
factor-1α gene. A founder diabetes transgenic cloned pig was generated by SCNT and
treated with insulin to allow for normal growth to maturity, at which point
epididymal sperm could be collected for cryopreservation. In vitro
fertilization and intrafallopian insemination using the cryopreserved epididymal
sperm resulted in diabetes model transgenic offspring. These results suggest that
artificial reproductive technology using cryopreserved epididymal sperm could be a
practical option for proliferation of genetically modified disease model pigs. 相似文献