Moisture conservation for rainfed wheat production with alternative mulches and conservation tillage in the hills of north-west India

In the hills of north–west India, maize (Zea mays L.)-wheat (Triticum aestivum L.) is the dominant cropping system. However, rainfed wheat suffers from lack of optimum moisture at sowing. Field experiments were conducted for 3 years on a silty clay loam (Typic Hapludalf) to evaluate the effectiveness of mulches and conservation tillage for rainfed wheat in mitigating this problem. The treatments were ten factorial combinations of five mulch-tillage practices and two nitrogen levels (N₆₀ and N₁₂₀ kg ha⁻¹). Mulch treatments consisted of application of 10 Mg ha⁻¹ (dry weight basis), to previous standing maize, of either wild sage (Lantana camara L.) or eupatorium (Eupatorium adenophorum Sprengel) in combination with either conventional or conservation (minium) tillage prior to wheat sowing. These alternative practices were compared to the conventional farmer practice of soil tillage after harvest of maize with no mulch. The application of these weed mulches to standing maize maintained friable soil structure owing to a five fold higher mean population of earthworms underneath mulch. Mulches resulted in 0.06–0.10 m³ m⁻³ higher moisture in the seed-zone when wheat was sown compared with the conventional farmer practice of soil tillage after maize harvest. Mulch-conservation tillage treatments favourably moderated the hydro-thermal regime for growing a wheat crop. The mean root mass density under these treatments at wheat flowering was higher by 1.27–1.40 times over the conventional farmer practice during the 3 year study. Conservation tillage holds promise because it does not require elaborate tillage and may ultimately reduce animal draught in the hilly regions. Recycling available organic materials having no fodder value coupled with conservation tillage may help enrich the soil environment in the long-term. The practice also offers gainful use of these obnoxious weeds that cause great menace in grass and forest lands in the region.     

Evaluation of in vitro chemosensitivity of vaccine-associated feline sarcoma cell lines to vincristine and paclitaxel

OBJECTIVE: To determine the in vitro sensitivity of 4 vaccine-associated feline sarcoma (VAFS) cell lines to the chemotherapeutic agents vincristine and paclitaxel. SAMPLE POPULATION: Cell lines derived from 4 VAFS specimens. PROCEDURES: Cell lines were cultured in vitro and individually exposed to various concentrations of vincristine and paclitaxel. Survival was estimated after 24 and 72 hours of exposure to each drug, and the drug concentrations that resulted in 50 and 90% reduction in number of viable cells (IC50 and IC90, respectively) were calculated. RESULTS: Both vincristine and paclitaxel had significant dose-dependent effects on the viability of the VAFS cell lines. After 72 hours of drug exposure, the IC50 and IC90 of vincristine for the 4 cell lines were between 0.005 to 0.039 microg/ml and 0.045 to 1.027 microg/ml, respectively. The IC50 and IC90 values for paclitaxel were between 0.037 to 0.092 microg/ml and 2.450 to 15.413 microg/ml, respectively. CONCLUSIONS: Results of pharmacokinetic studies on vincristine and paclitaxel in other species suggest that concentrations greater than the IC50 values may be possible for both drugs in feline patients as well. The drug concentrations at which viable cell numbers were reduced by 90% may also be attained in vivo for some cases, but detailed information is needed regarding the distribution, concentration, duration of availability, and toxicity of various drugs in cats. Carefully chosen combinations of antineoplastic agents need to be screened to identify treatment protocols that may be further evaluated clinically for the treatment of VAFS.     

Detection of tricresyl phosphates and determination of tri-o-cresyl phosphate in edible oils

Tricresyl phosphate (TCP) in contaminated edible oils was extracted using acetonitrile and detected by thin layer chromatography as well as gas chromatography (GC). The chromatoplate was developed with isooctane-ethyl acetate (90 + 10) and visualized by spraying with 2,6-dichloroquinone chloroimide. TCP gives a characteristic blue-violet spot when heated at 100 degrees C for 15 min. The method is direct and sensitive and can be used to detect as low as 2.5 micrograms TCP or TOCP (tri-o-cresyl phosphate). GC was carried out using 10% OV-101 as the stationary phase and flame ionization detection for confirmation and quantitation of TOCP in oils.     

Comparison of drip and sprinkler irrigation strategies on sunflower seed and oil yield and quality under Mediterranean climatic conditions

This study compares the effects of different irrigation regimes on seed yield and oil yield quality and water productivity of sprinkler and drip irrigated sunflower (Helianthus annus L.) on silty-clay-loam soils in 2006 and 2007 in the Mediterranean region of Turkey. In sprinkler irrigation a line-source system was used in order to create gradually varying irrigation levels. Irrigation regimes consisted of full irrigation (I₁) and three deficit irrigation treatments (I₂, I₃ and I₄), and rain-fed treatment (I₅). In the drip system, irrigation regimes included full irrigation (FI-100), three deficit irrigation treatments (DI-25, DI-50, DI-75), partial root zone drying (PRD-50) and rain-fed treatment (RF). Irrigations were scheduled at weekly intervals both in sprinkler and drip irrigation, based on soil water depletion within a 0.90 m root zone in FI-100 and I₁ plots. Irrigation treatments influenced significantly (P < 0.01) sunflower seed and oil yields, and oil quality both with sprinkler and drip systems. Seed yields decreased with increasing water stress levels under drip and sprinkler irrigation in both experimental years. Seed yield response to irrigation varied considerably due to differences in soil water contents and spring rainfall distribution in the experimental years. Although PRD-50 received about 36% less irrigation water as compared to FI-100, sunflower yield was reduced by an average of 15%. PRD-50 produced greater seed and oil yields than DI-50 in the drip irrigation system. Yield reduction was mainly due to less number of seeds per head and lower seed mass. Soil water deficits significantly reduced crop evapotranspiration (ET), which mainly depends on irrigation amounts. Significant linear relationships (R² = 0.96) between ET and oil yield (Y) were obtained in each season. The seed yield response factors (ky_seed) were 1.24 and 0.86 for the sprinkler and 1.19 and 1.06 for the drip system in 2006 and 2007, respectively. The oil yield response factor (ky_oil) for sunflower was found to be 1.08 and 1.49 for both growing seasons for the sprinkler and 1.36 and 1.25 for the drip systems, respectively. Oil content decreased with decreasing irrigation amount. Consistently greater values of oil content were obtained from the full irrigation treatment plots. The saturated (palmitic and stearic acid) and unsaturated (oleic and linoleic acid) fatty acid contents were significantly affected by water stress. Water stress caused an increase in oleic acid with a decrease in linoleic acid contents. The palmitic and stearic acid concentrations decreased under drought conditions. Water productivity (WP) values were significantly affected by irrigation amounts and ranged from 0.40 to 0.71 kg m⁻³ in 2006, and from 0.69 to 0.91 kg m⁻³ in 2007. The PRD-50 treatment resulted in the greatest WP (1.0 kg m⁻³) and irrigation water productivity (IWP) (1.4 kg m⁻³) in both growing seasons. The results revealed that under water scarcity situation, PRD-50 in drip and I₂ in sprinkler system provide acceptable irrigation strategies to increase sunflower yield and quality.     

PCR-based detection of an emerging avian pneumovirus in US turkey flocks.

Avian pneumovirus (APV) or turkey rhinotracheitis virus (TRTV) is an important respiratory pathogen of domesticated poultry in many countries in Europe, Africa, and Asia. Until recently, the United States was considered free of APV. In late 1996, an atypical upper respiratory tract infection appeared in turkey flocks in Colorado and shortly thereafter in turkey flocks in Minnesota. An avian pneumovirus (APV-US) that was serologically distinct from the previously described TRTV was isolated as the primary cause of the new syndrome. The nucleotide sequence of a fragment of the APV-US fusion gene was determined and used to develop a polymerase chain reaction-based assay that specifically detects APV-US viral nucleic acid sequences in RNA extracts of tracheal swabs and turbinate homogenates. The assay is highly sensitive in that it can detect <0.01 TCID50 of APV. The availability of this assay enables the rapid and accurate determination of APV-US in infected poultry flocks.     

Lowered vaccine dose for immunisation of calves against tropical theileriosis usingTheileria annulata lymphoblasts

Tropical Animal Health and Production - Bovine tropical theileriosis is of high incidence in calves below 2 months old in India. TheTheileria cell culture vaccine evolved by the Punjab Agricultural...