Preharvest applications of growth regulators and their effect on postharvest quality of table grapes during cold storage

Over 54,600 ha of table grapes (Vitis vinifera), mainly cvs. ‘Thompson Seedless’, ‘Flame Seedless’ and ‘Redglobe’, are planted in Chile. Almost the entire production is exported to the USA, Europe, Asia, or one of several Latin American countries, which typically requires 15–40 d of maritime transportation. During this period, several physical, physiological, and pathological factors cause table grape deterioration. Because berry size is the main quality factor in international markets, farmers often overuse the growth regulators, gibberellic acid (GA₃) and forchlorfenuron (CPPU), in an effort to increase berry size. We examined the effect of preharvest growth regulators on seedless (‘Thompson Seedless’, and ‘Ruby Seedless’) and seeded (‘Redglobe’) table grape cultivars during cold (0 °C) storage plus a shelf life period of 3 d at 20 °C. The overuse of GA₃, eight instead of two GA₃ applications on Thompson Seedless, and the use of one GA₃ application on Redglobe and ‘Ruby Seedless’, increased berry pedicel thickness and lowered cuticle content but induced shatter and predisposed grapes to gray mold caused by Botrytis cinerea. In contrast, CPPU increased berry pedicel thickness and cuticle content but did not increase shatter or gray mold incidence. Clusters that were subjected to overuse of combined GA₃ and CPPU were highly sensitive to shatter, had the thickest pedicel, and developed a high gray mold incidence during cold storage. Hairline, a fine cracking developed during cold storage, was induced on ‘Thompson Seedless’ and ‘Ruby Seedless’ by growth regulators, but no hairline occurred on ‘Redglobe’ table grapes. Therefore, berry quality during cold storage is greatly influenced by growth regulator management in the vineyard.     

Blood parasites of sheep in the Netherlands. I. Anaplasma mesaeterum sp.n. (Rickettsiales, Anaplasmataceae)

Summary On two occasions an anaplasm was isolated from sheep on the Dutch island of Ameland. The organism proved to be highly pathogenic for splenectomised sheep; a non-splenectomised animal recovered spontaneously after the packed cell volume had decreased by 40%. Treatment with oxytetracycline was effective. Its pathogenicity for goats appeared to be low, and the organism was apparently not infective to splenectomised cattle. This anaplasm differs from Anaplasma ovis in that less than 30% of the organisms are marginally situated in the red cell, as against over 70% in A. ovis; cross-immunity with A. ovis was incomplete and the latter appeared to be far more pathogenic to goats than the Dutch anaplasm, for which the name Anaplasma mesaeterum sp.n. is proposed. Its ultrastructure is similar to that of A. marginale and A. ovis. The vector is either Ixodes ricinus or Haemaphysalis punctata. Its practical importance remains to be ascertained.     

结球莴苣“烧边”成因及其调控措施的研究进展

结球莴苣烧边病是生理性病害，严重影响其品质，制约生产。国内外多数研究者认为钙缺乏导致结球莴苣烧边病的发生，但是现在许多研究表明其他因素也能导致烧边病的出现，例如环境因素、生长速率、内源激素等，所以研究烧边病发生的机制及其与环境因素的关系是防御结球莴苣烧边病发生的关键。     

Effects of fosinopril,captopril and valsartan on the expressionof tissue factor in human monocytes

AIM: To investigate the effects of angiotensin-converting enzyme inhibitors (ACEI), fosinopril, captopril and angiotensin II AT₁ antagonists, valsartan on tissue factor (TF) expression on monocytes induced by lipopolysaccharide (LPS). METHODS: Mononuclear leukocytes from normal delivered female umbilical veins were incubated with bacterial LPS in presence or absence of different ACE inhibitors .At the end of incubation, the cells were disrupted by 3 freeze-thaw cycles. TF procoagulant activity was assessed by a one-stage clotting assay. RT-PCR was used to check TF mRNA expression, and GAPDH mRNA was used for parallel assay. RESULTS and CONCLUSION: The results showed that increased expression of TF mRNA induced by LPS was inhibited by fosinopril, captopril and valsartan, respectively, and the procoagualant activity of monocytes was also reduced.     

鞭角华扁叶蜂体内多胺的动态

用紫外荧光法测定鞭角华扁叶蜂末龄幼虫及滞育预蛹血淋巴中多胺的动态.结果表明:鞭角华扁叶蜂血淋巴中含有8种多胺,其中腐胺(Put)、亚精胺(Spd)、精胺(Spm)、尸胺(Cad)为普遍存在的多胺,其发育的不同阶段,多胺的种类和含量均发生变化;在滞育预蛹的血淋巴中Spm一直保持较高的含量,并在整个滞育期间存在2个峰值;在滞育的前5个月中血淋巴中不存在Spd,之后Spd突然出现,且含量迅速升高;Cad的代谢较平稳,在化蛹前稍有下降;Put的含量除在滞育后3个月左右有较大的峰值外,其余阶段的含量均较高,并在化蛹前有升高的趋势.总之,多胺可能参与鞭角华扁叶蜂预蛹滞育过程.     

达乌里胡枝子花粉萌发特性研究

为揭示达乌里胡枝子(Lespedeza daurica)花粉的育性并为其花粉活力检测提供一种高效方法,本试验以达乌里胡枝子新鲜花粉为试验材料,采用液体培养基,研究了蔗糖、硼酸、氯化钙浓度、采样时间、温度、光照和培养时间等因素对花粉离体萌发和花粉管生长的影响.试验结果表明:单因素试验中,适宜花粉萌发的培养基配方为20 g...     

利用SSR标记分析高粱属种质资源的遗传多样性

本研究利用15对SSR 引物对161 份高粱属种质资源进行了遗传多样性分析。结果显示,15对引物共扩增出118条谱带,其中88条具有多态性,多态性比率(P)为75.21%;多态性信息含量(PIC值)变幅为0.612~0.806,平均为0.699。161份高粱属材料遗传相似性系数为0.636~0.977,平均基因多样性指数(H)为0.696,Shannon信息指数(I)为1.393。UPGMA聚类分析显示, 161 份高粱属材料在遗传相似系数(GS)为0.682处可分为3组;129份高粱和苏丹草材料在相似系数为0.671处可分为2组。表明SSR标记可以作为高粱属种间以及种内遗传分化分析的有力工具,被分析的高粱属种质材料具有较高的遗传多样性。     

Candidate genes underlying QTL for flowering time and their interactions in a wide spring barley (Hordeum vulgare L.) cross

Response to vernalization and photoperiod are the main determinants controlling the time to flowering in temperate cereals. While the individual genes that dete...     

Clostridium piliforme infection (Tyzzer disease) in horses: retrospective study of 25 cases and literature review

Tyzzer disease (TD) is caused by Clostridium piliforme, a gram-negative and obligate intracellular bacterium. The disease occurs in multiple species. A triad of lesions, namely colitis, hepatitis, and myocarditis, is described in cases of TD in some species, such as rats and mice. We carried out a retrospective analysis of 25 equine cases with a diagnosis of TD; 24 of 25 cases occurred in foals <45 d old; the remaining foal was 90 d old. There were 12 males and 12 females; no sex information was available for one foal. The affected breeds were Quarter Horse, Thoroughbred, Arabian, Paint, and Hanoverian. Most of the cases (19 of 25) occurred in the spring. There were 9 cases of sudden death; the remaining animals had diarrhea, fever, distended abdomen, depression, weakness, non-responsiveness, and/or recumbency. Gross findings included icterus, hepatomegaly with acinar pattern, serosal hemorrhages, pulmonary edema, and/or fluid content in small and large intestine. Microscopically, all foals had severe, multifocal, necrotizing hepatitis. Necrotizing lymphohistiocytic colitis was observed in 10 of 25 foals, and multifocal necrotizing myocarditis was found in 8 of 25. Gram-negative, Steiner-positive, intracytoplasmic filamentous bacteria were observed in hepatocytes, enterocytes, and myocardiocytes, respectively. PCR detected C. piliforme DNA in the liver (24 of 24), colon (20 of 24), and heart (5 of 25). Our results indicate that necrotic hepatitis is the hallmark of TD in horses; the so-called triad of lesions is not a consistent characteristic of the disease in this species.     

鸡白细胞抗菌肽的体外抗菌活性比较

应用氯化铵裂解、超声波破碎、离子交换层析和SDS—PAGE电泳等方法,对不同品种鸡白细胞中的抗菌肽成分进行了初步提取和分离,再用反相高效液相色谱法进一步分离和提纯．经微量琼脂糖弥散法初步检测体外抗菌活性后,利用微量稀释培养法分别检测了粗提抗菌肽对7个受试菌株的最小抑菌浓度（M1C）．结果表明,在鸡白细胞颗粒中存在着天然抗微生物活性物质,对革兰氏阳性细菌、革兰氏阴性细菌和真菌均具有抑制活性,且不同品种鸡粗提抗菌肽的体外抑菌浓度存在显著差异,其中罗曼褐壳蛋鸡粗提抗菌肽的抗菌活性最高,其次是三黄肉鸡,而罗斯308肉鸡较差．