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Tithipong PLANGSANGMAS Hathaipat RATTANATHANYA Wanlaya TIPKANTHA Saowaphang SANANNU Umaporn MAIKAEW Jessada THAEONOEN Bencharong SANGKHARAK Pornpiroon CHINSON Watcharin HIN-ON Nathavut KANATIYANONT Boripat SIRIAROONRAT Nantarika CHANSUE Parntep RATANAKORN 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):181
The plains zebra (Equus quagga) is a zebra species commonly kept in zoos around the world. However, they are not tame like their domestic relatives and are difficult to immobilize. We immobilized 30 captive plains zebra with a combination of etorphine hydrochloride (2–4 mg), acepromazine (8 mg), and xylazine hydrochloride (30 or 50 mg) to perform physical examination and blood sample collection for disease diagnostics. Physiological parameters including heart rate, respiratory rate, body temperature, and hemoglobin oxygen saturation were recorded. All zebras exhibited satisfactory anesthesia and fully recovered without re-narcotization. The results suggest that etorphine hydrochloride-acepromazine-xylazine hydrochloride combination for plains zebra immobilization is a safe and sufficient regimen for short procedures such as wellness examinations and sample collection. 相似文献
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The plant Asparagus racemosus is one of the most widely used sources of phytoestrogens because of its high content of the steroidal saponins, shatavarins I-IV, in roots. The dry root of A. racemosus, known as "Rak-Sam-Sip" in Thai, is one of the most popular herbal medicines, used as an anti-inflammatory, an aphrodisiac and a galactagogue. Recently, the interest in plant-derived estrogens has increased tremendously, making A. racemosus particularly important and a possible target for fraudulent labeling. However, the identification of A. racemosus is generally difficult due to its similar morphology to other Asparagus spp. Thus, accurate authentication of A. racemosus is essential. In this study, 1557-bp nucleotide sequences of the maturase K (matK) gene of eight Asparagus taxa were analyzed. A phylogenetic relationship based on the matK gene was also constructed. Ten polymorphic sites of nucleotide substitutions were found within the matK sequences. A. racemosus showed different nucleotide substitutions to the other species. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the matK gene was developed to discriminate A. racemosus from others. Only the 650-bp PCR product from A. racemosus could be digested with BssKI into two fragments of 397 and 253-bp while the products of other species remained undigested. Ten commercially crude drugs were analyzed and revealed that eight samples were derived from A. racemosus while two samples of that were not. Thus, the PCR-RFLP analysis of matK gene was shown to be an effective method for authentication of the medicinally phytoestrogenic species, A. racemosus. 相似文献
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Suttada Ruangsuttapha Klaus Eimert Max-Bernhard Schröder Benchamas Silayoi Jessada Denduangboripant Kamnoon Kanchanapoom 《Genetic Resources and Crop Evolution》2007,54(7):1565-1572
Musa acuminata Colla (AA genomes) and Musa balbisiana Colla (BB genomes) are the wild progenitors of the cultivated banana, they are highly variable in Thailand. The genetic system
is relatively unknown and complicated due to interspecific hybridization, heterozygosity and polyploidy, which are common
in most clones. These factors make identification of closely related banana cultivars difficult, especially when sterile.
The high annealing temperature-random amplified polymorphic DNA (RAPD) technique was used to estimate the genetic relationship
between 22 selected banana cultivars, utilizing 14 random primers. Phylogenetic relationship was determined by unweighted
pair group method with arithmetical averages cluster analysis. The dendrogram constructed from the similarity data showed
that all the 22 cultivars analysed were closely related with a narrow genetic base. There were sufficient RAPD polymorphisms
that were collectively useful in distinguishing the cultivars. The dendrogram grouped all the AA, BB, AAA, AAB and ABB genomes
into a major cluster. Several subgroups are recognized within the major clade. As expected, Ensete glauca Roxb. (Musaceae) and Strelitzia reginae Banks (Strelitziaceae) were clearly differentiated from the analysed edible bananas. Our study showed that RAPD markers are
sufficiently abundant to classify and readily dissect genetic differences between the closely related Musa germplasm and provide a basis for the selection of parents for improvement of this germplasm. 相似文献
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