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1.
Congenital lobar emphysema was found in a three-month-old male springer spaniel. Clinical signs included coughing, progressive dyspnoea and abnormal lung auscultation. Radiographic examination revealed overinflation and hyperlucency of the right middle lung lobe. Following lung lobectomy, clinical signs resolved and the animal had normal exercise tolerance. Previous case reports are reviewed and discussed in relation to human congenital lobar emphysema. 相似文献
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Nicole Schneider Marianne Heimann Jean-Philippe Lejeune Denis R.V.G. Verwilghen Ginette P. Deby-Dupont Didier A. Serteyn 《Journal of veterinary science (Suw?n-si, Korea)》2006,7(1):83-85
In the human and equine species, different kinds of free floating intra-articular particles are related to certain disorders. Osteochondral fragments formed during osteochondrosis dissecans are the most common finding in the equine species, whereas in humans rice bodies due to rheumatoid arthritis are more frequent. Herein we report a third type of floating body inside the stifle of an adult draught horse stallion, in macroscopic appearance similar to articular rice bodies known in humans. As revealed by histologic examination, the two particles consist of polypoid degenerated structures derived from synovial villi. Their formation was probably induced by ischemia. 相似文献
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FlordeFátima Rosas-Cárdenas Noé Durán-Figueroa Jean-Philippe Vielle-Calzada Andrés Cruz-Hernández Nayelli Marsch-Martínez Stefan de Folter 《Plant methods》2011,7(1):4
Background
Small RNAs emerged over the last decade as key regulators in diverse biological processes in eukaryotic organisms. To identify and study small RNAs, good and efficient protocols are necessary to isolate them, which sometimes may be challenging due to the composition of specific tissues of certain plant species. Here we describe a simple and efficient method to isolate small RNAs from different plant species. 相似文献4.
Sandra Dèrozier Franck Samson Jean-Philippe Tamby Cécile Guichard Véronique Brunaud Philippe Grevet Séverine Gagnot Philippe Label Jean-Charles Leplé Alain Lecharny Sébastien Aubourg 《Plant methods》2011,7(1):1-10
Background
Efficient high throughput screening systems of useful mutants are prerequisite for study of plant functional genomics and lots of application fields. Advance in such screening tools, thanks to the development of analytic instruments. Direct analysis in real-time (DART)-mass spectrometry (MS) by ionization of complex materials at atmospheric pressure is a rapid, simple, high-resolution analytical technique. Here we describe a rapid, simple method for the genetic discrimination of intact Arabidopsis thaliana mutant seeds using metabolic profiling by DART-MS.Results
To determine whether this DART-MS combined by multivariate analysis can perform genetic discrimination based on global metabolic profiling, intact Arabidopsis thaliana mutant seeds were subjected to DART-MS without any sample preparation. Partial least squares-discriminant analysis (PLS-DA) of DART-MS spectral data from intact seeds classified 14 different lines of seeds into two distinct groups: Columbia (Col-0) and Landsberg erecta (Ler) ecotype backgrounds. A hierarchical dendrogram based on partial least squares-discriminant analysis (PLS-DA) subdivided the Col-0 ecotype into two groups: mutant lines harboring defects in the phenylpropanoid biosynthetic pathway and mutants without these defects. These results indicated that metabolic profiling with DART-MS could discriminate intact Arabidopsis seeds at least ecotype level and metabolic pathway level within same ecotype.Conclusion
The described DART-MS combined by multivariate analysis allows for rapid screening and metabolic characterization of lots of Arabidopsis mutant seeds without complex metabolic preparation steps. Moreover, potential novel metabolic markers can be detected and used to clarify the genetic relationship between Arabidopsis cultivars. Furthermore this technique can be applied to predict the novel gene function of metabolic mutants regardless of morphological phenotypes. 相似文献5.
In the UK, critical loads have been mapped for both soils and freshwaters and the maps indicate that discrepancies may occur between these two receptors over sensitive areas of the UK. Freshwater critical load maps were prepared by calculating the Henriksen critical load for the most sensitive water body in each 10 km grid square. Critical loads for soils were calculated according to the mineralogy and associated soil properties of the dominant soil at a 1 km resolution. To examine the differences between the soil and freshwater data sets it is necessary to calculate critical loads at a smaller scale using the catchment as the focus for study. This was done by selecting a catchment on granitic parent material in the North of Scotland. Data on water chemistry, collected on a weekly basis, was used to calculate temporal variations in critical loads for freshwaters using the Henriksen method. Soil sampling across the catchment was conducted on a grid based system to provide estimates of spatial variability in sensitivity. Profile characteristics and soil chemical data obtained from detailed soil sampling programmes were used in the PROFILE model to determine the spatial variation in critical loads for soils. In general, the results show that the critical loads for soils tend to be lower than those for freshwater. The spatial variation in the soil critical load tends to be small whilst the temporal variation in critical load for freshwaters is large. In order to account for these differences it is important to identify the key processes within the catchment which play a major role in controlling streamwater chemistry. This procedure improves the relationship between critical loads for soils and waters. 相似文献
6.
Deng H Le Rhun D Buffet JP Cotté V Read A Birtles RJ Vayssier-Taussat M 《Veterinary research》2012,43(1):15
ABSTRACT: Numerous mammal species, including domestic and wild animals such as ruminants, dogs, cats and rodents, as well as humans, serve as reservoir hosts for various Bartonella species. Some of those species that exploit non-human mammals as reservoir hosts have zoonotic potential. Our understanding of interactions between bartonellae and reservoir hosts has been greatly improved by the development of animal models for infection and the use of molecular tools allowing large scale mutagenesis of Bartonella species. By reviewing and combining the results of these and other approaches we can obtain a comprehensive insight into the molecular interactions that underlie the exploitation of reservoir hosts by Bartonella species, particularly the well-studied interactions with vascular endothelial cells and erythrocytes. 相似文献
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Courant F Antignac JP Laille J Monteau F Andre F Le Bizec B 《Journal of agricultural and food chemistry》2008,56(9):3176-3184
In the present study, the occurrence of the main sex steroid hormones in milk, egg, and meat was evaluated on the basis of a highly specific gas chromatography-tandem mass spectrometry measurement method. Globally, the results indicated that targeted estrogens and androgens occurred at similar levels (concentration levels in the 10-100 ng kg (-1) range) in the analyzed muscle and milk samples. The same compounds occurred at about 10-fold higher concentrations (i.e., in the 100-1000 ng kg (-1) range) in eggs and kidney samples. More precisely, egg and milk appeared as a non-negligible sources of estradiol (i.e., 2.2 +/- 0.8 and 3.1 +/- 2.0 ng day (-1), respectively), whereas testosterone exposure is caused by ingestion of meat and/or egg (i.e., 12.2 +/- 48.2 and 5.2 +/- 2.3 ng day (-1), respectively). The provided exposure data will be further exploited in the scope of a risk assessment study regarding endocrine disruption associated with these molecules. 相似文献
9.
Assessing the effects of the clumping phenomenon on BRDF of a maize crop based on 3D numerical scenes using DART model 总被引:2,自引:0,他引:2
Sylvie Duthoit Valrie Demarez Jean-Philippe Gastellu-Etchegorry Emmanuel Martin Jean-Louis Roujean 《Agricultural and Forest Meteorology》2008,148(8-9):1341-1352
Inverting radiative transfer (R-T) models against remote sensing observations to retrieve key biogeophysical parameters such as leaf area index (LAI) is a common approach. Even if new inversion techniques allow the use of three-dimensional (3D) models for that purpose, one-dimensional (1D) models are still widely used because of their ease of implementation and computational efficiency. Nevertheless, they assume a random distribution of foliage elements whereas most canopies show a clumped organization. Due to that crude simplification in the representation of the canopy structure, sizeable discrepancies can occur between 1D simulations and real canopy reflectance, which may further lead to false LAI values. The present investigation aims to appraise to which extent the incorporation of a clumping index (noted λ) into 1D R-T model could improve the simulations of Bidirectional Reflectance Distribution Function (BRDF). Canopy BRDF is simulated here for three growth stages of a maize crop with the Discrete Anisotropic Radiative Transfer (DART) model in the visible and near infrared spectral bands, for two contrasted soil types (dark and bright) and different levels of heterogeneity to represent the canopy structure. 3D numerical scenes are based on in-situ structural measurements and associated BRDF simulations are thus considered as references. 1D scenarios assume either that leaves are randomly distributed (λ = 1) or clumped (λ < 1). If BRDF simulations seem globally reliable under the assumption of a random distribution in near infrared, it can also lead to relative errors on the total BRDF up to 30% in the red spectral band. It comes out that the use of a clumping index in a 1D reflectance model generally improves BRDF simulations in the red considering a bright soil, which seems relatively independent of LAI. In the near infrared, best results are usually obtained with homogeneous canopies, except with the dark soil. Clearly, influent factors are mainly the LAI and the spectral contrast between soil and leaves. 相似文献
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