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1.
KM Morton SL Catt FK Hollinshead WMC Maxwell G Evans 《Reproduction in domestic animals》2004,39(6):454-461
Cumulus-oocyte complexes from hormone-stimulated 3-4-week-old (n=43) and 6-7-week-old (n=12) prepubertal lambs were matured in vitro and incubated with unsorted, or X- or Y-spermatozoa separated with a high-speed cell sorter (SX MoFlo)frozen-thawed. Presumptive zygotes were then cultured to the blastocyst stage, and transferred to recipients fresh or after cryopreservation (frozen). Oocyte cleavage was higher (p <0.05) with unsorted (515/926, 55.6%) than X- or Y-spermatozoa (261/672, 38.8% and 229/651, 35.2%, respectively) and blastocyst formation (% zygotes) by Day 9 of in vitro culture was lower (p <0.05) for X- (102/261, 39.1%) than unsorted spermatozoa (249/515, 48.3%), but did not differ between Y-spermatozoa (103/229, 45.0%) and unsorted spermatozoa, or between X- and Y-spermatozoa (p >0.05). For fresh embryos, survival to term was 50.0% (3/6) for unsorted, 0.0% (0/6) for X- and 16.7% (1/6) for Y-spermatozoa-derived embryos (p >0.05), and for frozen embryos was 4.0% (2/50) for unsorted, 9.1% (2/22) for X- and 2.9% (1/34) Y-spermatozoa-derived embryos (p >0.05). Of the two lambs born from X-spermatozoa-derived embryos, one was female (50%), and from the two Y-spermatozoa-derived lambs, both were male (100%), demonstrating that lambs can be produced after the transfer of fresh and cryopreserved IVP embryos derived from prepubertal lamb oocytes and frozen-thawed sex-sorted sperm. 相似文献
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A mycosis in larval tiger prawn, Penaeus monodon Fabricius, is described for the first time from India. The hyphae of Lagenidium callinectes are contorted, irregularly branched, sparingly septate, and contain a cell wall and membrane, vacuoles, mito chondria, ribosomes, small and large vesicles, and Woronin bodies. The spores occur singly or in pairs. The fungal mycelium may either invade and embed itself in the tissues, or alternatively, replace all the muscle tissues of the infected larval P. monodon. Fungus infected, untreated populations of nauplii, zoea and myksis exhibited mortalities of 5.33 ± 0.55%, 24.68 ± 4.58% and 47.89 ± 0.27%, respectively. A 0.5 ppm treatment with trifluralin significantly reduced the mortality of infected larval populations (i.e. 1.1% nauplii, 3.28% zoea and 5.21% myksis mortality). Lagenidium sp. exhibited growth in potato dextrose agar medium and in Sabouraud's agar at 28 °C. 相似文献
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KM Blackwood 《Science (New York, N.Y.)》1996,273(5277):909-912
Side-chain ferroelectric liquid crystalline polymers are currently used in a number of applications, including displays and electrical sensors. Comparisons with existing technologies and materials indicate that relative to ceramics, such polymers have lower figures of merit but offer greater durability in sensor applications. 相似文献
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D. Minoo V. N. Jayakumar S. S. Veena J. Vimala A. Basha K. V. Saji K. Nirmal Babu K. V. Peter 《Genetic Resources and Crop Evolution》2008,55(3):459-470
Vanilla is naturally distributed in Mexico and parts of Central America and the history of origin of cultivated vanilla suggests
that the entire stock outside Mexico may be from a single genetic source. In the present study, RAPD polymorphism was used
to estimate the level of genetic diversity and interrelationships among different collections of Vanilla planifolia Andr., and few related species, including both leafy and leafless types such as V. tahitensis J.W.Moore, V. andamanica, Rolfe, V. pilifera Holtt., and V. aphylla Blume. Studies revealed that there are very limited variation within collections of V. planifolia, indicative of its narrow genetic base, and of the related species we tested, V. tahitensis is nearest to V. planifolia. The species studied are diverse and have a similarity ranging from 1.2 to 57.3 %. Of the sampled taxa, V. andamanica is the most divergent and there is also reasonable variability within its collections, indicating the possibility of natural
seed set. A total of 82 polymorphic bands expressed in the RAPD profiles were used to generate a genetic distance matrix,
which was then used in cluster analysis. Specific groupings were revealed by the cluster analysis whereby the leafless forms
(V. aphylla, V. pilifera and the new species) and V. andamanica formed separate clusters. This is the first report of species interrelationship studies, including both cultivated and wild
vanilla species. 相似文献
5.
AK Biswas Pankaj Sharma KM Hati S Ramana AB Singh 《Communications in Soil Science and Plant Analysis》2018,49(17):2189-2196
Distilleries produce a huge quantity of effluents, popularly known as spent wash (SW), which when bio-methanated produce post-methanation effluents (PME). A field experiment on soybean–wheat system was conducted for five consecutive years in a Vertisol of central India to evaluate the effect of distillery effluent (DE) on soil carbon and nitrogen dynamics. Ten treatment combinations consisting of control, 100% NPK + Farmyard Manure (FYM), and graded level of SW and PME were applied. Total carbon content of soil increased significantly with applications of FYM and DE. SW was found superior in enhancing carbon content of soil in comparison to PME. Farmyard Manure contributed more carbon toward the recalcitrant pool, whereas DE contributed more carbon toward the active and slow pool. Nitrogen (N) availability was significantly improved with the application of DE. Balanced application of DE may act as amendment for increasing C and N stocks in Vertisol. 相似文献
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Secondary metabolites from Dysoxylum malabaricum and Dysoxylum beddomei were tested against mature and immature stage of the mosquito vector Anopheles stephensi under laboratory conditions. The triterpenes 3beta,24,25-trihydroxycycloartane and beddomeilactone from D. malabaricum and D. beddomei showed strong larvicidal, pupicidal and adulticidal activity. They also affected the reproductive potential of adults by acting as oviposition deterrents. The highest concentration tested (10 ppm) of both compounds evoked more than 90% mortality and oviposition deterrence. 相似文献
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The productivity of cotton is lowest in India and far below that of the world average productivity. Suitable management practices like intercropping and judicious combination of organic and inorganic manures are considered as yield improvement technologies and can avoid environmental pollution. However, which intercrop is suitable for the study area and what combination of nutrients will perform better needs to be investiaged. With this background and to test-verify the same, field experiments were conducted on cotton with split plot design to evaluate cotton based intercropping system along with nutrient management practices for enhancing the cotton productivity. Five intercropping systems viz., sole cotton, cotton + onion, cotton + blackgram, cotton + greengram and cotton + lucerne were included in the main plot. The subplot consisted of combinations of inorganic and organic manures namely, 100% recommended inorganic nitrogen (N), 75% inorganic N + 25% N through poultry manure, 75% inorganic N + 25% N through sunnhemp, 75% inorganic N + 25% N through farm wastes, 75% inorganic N + 25% N through weed compost. The results revealed that sole cotton followed by cotton + blackgram intercropping and 75% inorganic N + 25% N through poultry manure recorded better growth and yield attributes, higher cotton yield during both the years. Cotton + onion resulted in the maximum cotton equivalent yield of 2396 kg ha?1 followed by cotton + blackgram (2240 kg ha?1). Better N use efficiency and post-harvest soil available N, phosphorus (P) and potassium (K) was also associated with cotton + blackgram intercropping and 75% inorganic N + 25% N through poultry manure. Benefit: cost (BC) ratios were also higher in the same treatment. It could be concluded from these results that the cotton productivity is higher under the sole crop of cotton, however, the cotton equivalent yield is significantly higher with intercropping and these treatments proved that soil fertility status can be sustained with integrated plant nutrient management practices and intercropping systems. 相似文献