Peripartal Endocrinology in the Mare and Foetus

The endocrine profiles in the periparturient mares are dominated by increasing concentrations of progestagens and decreasing oestrogens. These hormones are produced by precursors from the foetus, metabolized by the placenta and act primarily on the maternal uterus. The circulating concentrations of hormones in maternal plasma, generally, represent a small proportion of those metabolized by the foetus and utero-placental tissues. There is clear evidence that the foetal hypothalamo-pituitary-adrenal (HPA) axis initiates the process of foetal maturation and the hormonal cascade which culminates in parturition at term. The endocrine changes associated with abnormal pregnancy and abortion in late pregnancy are less well understood, as are the hormonal treatments needed to avert these problems. Further work is needed to establish the biological role of the various hormones present in pregnant mares and, in particular, those hormones which control myometrial quiescence.     

Detection of Ureaplasma diversum in the upper airways of Australian feedlot cattle

Bovine respiratory disease (BRD) exerts a major impact on the beef cattle industry nationally and worldwide, with a range of aetiological factors impacting its pathogenesis. Previous research has focussed on an increasing number of bacteria and viruses that have been shown to play a role in eliciting disease. Recently, additional agents have been emerging as potential contributors to BRD, including the opportunistic pathogen Ureaplasma diversum. To determine if U. diversum was present in Australian feedlot cattle and if that presence was linked to BRD, nasal swabs were collected from a cohort of 34 hospital pen animals and compared to 216 apparently healthy animals sampled contemporaneously at feedlot induction and again after 14 days on feed at an Australian feedlot. All samples were subjected to a de novo polymerase chain reaction (PCR) assay targeting U. diversum in combination with other BRD agents. U. diversum was detected at a low prevalence in cattle at induction (Day 0: 6.9%, Day 14: 9.7%), but in a significantly greater proportion of cattle sampled from the hospital pen (58.8%). When considering the presence of other BRD-associated agents, co-detection of U. diversum and Mycoplasma bovis was most common in hospital pen animals receiving treatment for BRD. These findings suggest that U. diversum may be an opportunistic pathogen involved in the aetiology of BRD in Australian feedlot cattle, in combination with other agents, with further studies are warranted to identify if a causal relationship exists.     

Profile of Steroid Receptors and Increased Aromatase Immunoexpression in Canine Inflammatory Mammary Cancer as a Potential Therapeutic Target

Canine inflammatory mammary cancer (IMC) has been proposed as a model for the study of human inflammatory breast cancer (IBC). The aims of this study were to compare the immunohistochemical expression of aromatase (Arom) and several hormone receptors [estrogen receptor α (ERα), estrogen receptor β (ERβ), progesterone receptor (PR) and androgen receptor (AR)], in 21 IMC cases vs 19 non‐IMC; and to study the possible effect of letrozole on canine IMC and human inflammatory breast cancer (IBC) in vitro using IPC‐366 and SUM‐149 cell lines. Significant elevations of the means of Arom Total Score (TS), ERβ TS and PR TS were found in the IMC group (p = 0.025, p = 0.038 and p = 0.037, respectively). Secondary IMC tumours expressed higher levels of Arom than primary IMC (p = 0.029). Non‐IMC PR‐ tumours contained higher levels of Arom than non‐IMC PR+ tumours (p = 0.007). After the addition of letrozole, the number of IMC and IBC cells dropped drastically. The overexpression of Arom found and the results obtained in vitro further support canine IMC as a model for the study of IBC and future approaches to the treatment of dogs with mammary cancer, and especially IMC, using Arom inhibitors.     

MECHANICAL TREATMENT FOR IMPROVING UPLAND GRAZING

The botanical changes in a wet ( Molinia ) and a drier ( Nardtts ) upland pasture that followed a single defoliation by a forage harvester are briefly described. Without fertilizer the bare and litter-covered areas were soon recolonized by the original dominants; with lime and fertilizer the recolonization was mainly by Agrostis spp., even when Agrostis was a minor element in the original sward. These changes were markedly accelerated by the forage-harvester treatment. The results of surface seeding after the forage-harvester treatment are compared with those obtained after rotary cultivation. Contrasting moisture regimes and competitive thresholds led to considerable differences in sward balance and in establishment of sown species, and seeding was successful only on the wetter site. Too rapid regeneration of undesirable remnants was prevalent, but this and other hazards are related to the uneven deposit of litter left by the forage harvester; thick, slowly-rotting accumulations inhibited the redevelopment of rapidly-growing species and germination on seeded areas.     

Soil and erosion features of the central plateau region of Tigrai,Ethiopia

The results of reconnaissance soil surveys covering 6,000 km² are used to describe the Central Plateau region, which lies at elevations of 2,000 to 2,800 m in northern Ethiopia. Landform and soil sequences on calcareous shales, dolerites and sandstones are described, in which the principal soil units are Lithosols, Luvisols, Cambisols, Arenosols and Vertisols. Detailed morphological and analytical data are presented for a profile representative of arable soils in each sequence.Small-scale subsistence cultivation of cereals is the dominant land use; all land which is physically cultivable is at present cultivated. Settlement patterns are closely related to soil type, nucleated settlement occurring on fine textured soils but dispersed settlement on coarser textured and more freely draining soils.Erosion and soil moisture features of the three landforms described were investigated and compared. Empirical methods and suspended sediment measurements indicate high rates of regional soil loss (17–33 t ha^?1 yr^?1), accounted for by seasonally high rates of rainfall erosivity, steep terrain and poor land use. The recent development of gully erosion is seen to be linked to the disintegration of waterfall tufas. Application of the universal soil loss equation to arable lands indicates potential annual soil losses in the range of 400 t ha^?1 on Vertisols to 200 t ha^?1 on Cambisols: differences in rates are ascribed principally to differences in crop planting dates, which affect the degree of vegetative protection during periods of high rainfall erosivity.Soil moisture is shown to be in the available range for less than three months in the year. The time at which moisture in the profile enters the available range differed between the three soils monitored and was found to be closely related to the crop planting date, thus indirectly affecting the erosion hazard.     

Aetiology and diagnosis of persistent nasal disease in the dog: a retrospective study of 42 cases

Forty-two dogs with a history of persistent nasal disease were evaluated by a combination of clinical examination, thoracic and nasal radiography, retroflexed endoscopy and biopsy, and anterograde rhinoscopy and blind nasal biopsy. A definitive diagnosis was made in 91 per cent of cases. Neoplasia was the most common diagnosis (33 per cent of cases), followed by inflammatory rhinitis (24 per cent). Other diagnoses included periodontal disease (10 per cent), aspergillosis (7 per cent) and foreign bodies (7 per cent). Adenocarcinoma was the most common tumour diagnosed. The clinical findings were found to be too variable to be used as specific diagnostic criteria. Anterograde rhinoscopy and retroflexed endoscopy had higher specificity and sensitivity than radiology for the diagnosis of neoplasia, inflammatory rhinitis, aspergillosis and foreign bodies. With a systematic approach to the investigation of persistent nasal disease, a definitive diagnosis can be successfully obtained in the vast majority of cases.     

Resolution of skin lesions and long-term survival in a dog with superficial necrolytic dermatitis and liver cirrhosis

A nine-year-old, neutered female Shetland sheepdog was presented with crusted, ulcerative skin lesions affecting the footpads, commissures of the lips and the lateral canthi of the eyes. Histopathological examination of skin biopsies revealed changes consistent with superficial necrolytic dermatitis and biochemical analysis demonstrated elevated liver enzymes. Abdominal radiography revealed a small liver which, on ultrasonography, appeared diffusely mottled and showed changes suggestive of periportal fibrosis. On exploratory laparotomy, the pancreas appeared normal, but the liver was small and had multiple nodules throughout the parenchyma. This appearance was confirmed as cirrhosis on histopathological examination. The dog was placed on a hepatic support diet and treated with colchicine, essential fatty acid supplementation and raw egg yolks. After four weeks, the skin lesions had resolved and the dog remained free of clinical signs over a 22-month follow-up period.