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调查了曾经暴发沙门氏菌病的四个猪场的14头病猪,发现在这些猪的肿胀淋巴结中出现带有淋巴细胞排空的肉芽肿性炎症。应用免疫标记和PCR方法在病变部位检测到猪圆环病毒2(PCV2)抗原和PCV2DNA。此外,在这些病猪的肺脏中检测出猪繁殖与呼吸综合征病毒(PRRSV),分离到猪霍乱沙门氏菌。在9头沙门氏菌感染猪中,有5头为沙门氏菌、PMWS与PRRSV并发感染,其数量(55.6%)远远高于沙门氏菌与PMWS感染猪(22.2%)或沙门氏菌与PRRSV感染猪(22.2%)。 相似文献
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Haruyuki HIRATA Temidayo OMOBOWALE Olufunke ADEBAYO Nodoka ASANUMA Asako HARAGUCHI Yoshiki MURAKAMI Kodai KUSAKISAKO Keiko IKEDA Mitsuhiko ASAKAWA Kazuyuki SUZUKI Chiaki ISHIHARA Hiromi IKADAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(3):338
The present study examined the presence of Babesia parasites in 104 domestic dogs in Nigeria. Sequentially, Babesia parasites infecting domestic dogs underwent genetic and phylogenetic analyses. The results of nested PCR based on the Piroplasmida 18S rRNA gene illustrated that 13.5% (14/104) of the samples were positive. The obtained positive samples determined the nucleotide sequences of the 18S rRNA genes. In the genetic and phylogenetic analyses, four of five nucleotide sequences were similar to Babesia canis rossi, and one sample exhibited a close similarity to a Babesia sp. isolated from a raccoon in Hokkaido, Japan. The present study revealed the widespread presence of B. canis rossi among domestic dogs in Nigeria. 相似文献
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Rieko OKAME Keiko NAKAHARA Noboru MURAKAMI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(7):815-821
We measured the plasma levels of amino acids at various reproductive stages in female
rats, including the estrous cycle, pregnancy and lactation, and compared the resulting
amino acid profiles using two- or three-dimensional figures. These figures revealed that
the amino acid profiles of pregnant and lactating dams differed considerably from those
during the estrous cycle or in male rats. The plasma levels of individual amino acids were
almost the same between proestrus, estrus, metestrus and diestrus, and their profiles did
not differ significantly. However, the amino acid profiles changed during pregnancy and
lactation in dams. The plasma Ser level decreased significantly in mid and late pregnancy,
whereas Tyr, Gly and His decreased significantly in the late and end stages of pregnancy,
and Trp and Lys significantly decreased and increased at the end of pregnancy,
respectively. Much larger changes in amino acid profiles were observed during lactation,
when the levels of many amino acids increased significantly, and none showed a significant
decrease. Plasma Pro, Ser and Gly levels increased continuously from day 1 until day 15 of
lactation, whereas Asn and Met increased significantly from days 1 and 5 respectively
until the end of lactation. These results suggest that the profiles of plasma amino acids
show characteristic changes according to reproductive stage and that it may be necessary
to consider such differences when performing amino acid-based diagnosis. 相似文献
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Kohei MURAKAMI Tomohiro YONEZAWA Naoaki MATSUKI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1715-1717
Idiopathic polyarthritis (IPA) is a very common inflammatory arthropathy in the dog.
Canine IPA is diagnosed mainly by detecting increased number of leukocytes in the synovial
fluid (SF), which is easily influenced by glucocorticoid therapy. We obtained 31 SF
samples from 24 IPA dogs prior to (n=19) and/or after
(n=12) 1 to 10 weeks of glucocorticoid therapy. The SF total protein
concentrations of IPA dogs were significantly higher than those of dogs with non-arthritis
diseases (n=34) and healthy controls (n=10). Our data
revealed that the SF total protein concentrations are not influenced by several weeks of
glucocorticoid therapy. Hence, the SF total protein concentration is applicable as a
diagnostic marker of canine IPA even when the patients are receiving glucocorticoid
therapy. 相似文献
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Hiroshi FUJISE Masaru MURAKAMI Bhuminand DEVKOTA Ishwori Prasad DHAKAL Kumiko TAKEDA Hirofumi HANADA Hideo FUJITANI Masao SASAKI Kosaku KOBAYASHI 《Animal Science Journal》2003,74(1):1-5
Lulu is a dwarf cattle population bred in the Mustang district of western and central Nepal. This area is located around the habitat boundary between Bos taurus and Bos indicus. The peculiarities of Lulu are their small size (weight range in the adult female: 68–153 kg) and rearing in high mountain areas at 2800 m to 4000 m in altitude. There were 5770 head of Lulu cattle in the Mustang district in 1998, 4333 females and 1437 males. The morphological appearance of Lulu is Bos taurus. However, one of the five Lulu studied in Kagbeni, Mustang had a Bos indicus mitochondrial DNA type based on the D‐loop sequence, while the other four were Bos taurus. It is suggested that there are hybrids of Lulu with Bos indicus maternal lineage in a mostly taurine‐breed genetic background. Steps must be taken to preserve the unique Lulu. 相似文献
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Fujiko MINAMI-FUKUDA Makoto NAGAI Hikaru TAKAI Toshiaki MURAKAMI Tadashi OZAWA Shinobu TSUCHIAKA Sachiko OKAZAKI Yukie KATAYAMA Mami OBA Naomi NISHIURA Yukiko SASSA Tsutomu OMATSU Tetsuya FURUYA Satoshi KOYAMA Junsuke SHIRAI Hiroshi TSUNEMITSU Yoshiki FUJII Kazuhiko KATAYAMA Tetsuya MIZUTANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2013,75(12):1651-1655
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Takayuki FUJIWARA Keiichi MURAKAMI Eiji YOSHIDA Itaru TAKAHASHI 《Soil Science and Plant Nutrition》2009,55(2):309-314
The purpose of the present study was to evaluate the capability of near infrared spectroscopy (NIRS) as a simple method to monitor the lipid content of garbage compost, which is a potential inhibitor of plant growth. We conducted a cultivation experiment with vegetable mock pak choy ( Brassica rapa L. Parachinensis Group) using two application rates of four garbage composts that differed in lipid content. The input of lipid from the compost to the field showed a significant negative correlation with germination rate and plant height in the initial growth stage. Reflectance spectra of untreated and freeze-dried and milled compost samples were taken using a scanning monochromator. Second-derivative spectra and multiple regression analysis were used to develop calibration equations for lipid and moisture contents. The calibration was carried out with the short wavelength region ([SWR] 800–1100 nm) and the long wavelength region ([LWR] 1100–2500 nm) separately. The calibration equations with the LWR were more accurate than those with the SWR for lipid and moisture determinations. The accuracies of the calibration equations for untreated samples were comparable to those for freeze-dried and milled samples. In conclusion, we suggest that the application rate of garbage compost can be determined by measuring the lipid content of untreated samples by NIRS. 相似文献