Inheritance of Aluminum Tolerance and its Effects on Grain Yield and Grain Quality in Oats (Avena Sativa L.)

Summary Aluminum toxicity due to the cation Al⁺³ is a major factor limiting yields in acid soils. Wide genetic variability to aluminum tolerance is found in oat genotypes. The objectives of this study were to determine the number of genes controlling aluminum tolerance in oats and to verify if any detrimental effects were present of the aluminum tolerance genes on grain yield and grain quality in Al⁺³free soils. Aluminum tolerance was estimated as the average regrowth of the main root after exposure to toxic levels of Al⁺³ in a hydroponic solution under controlled conditions. The number of genes controlling that trait was estimated from the distribution of the average root regrowth frequencies in a population of 333 recombinant inbred lines (RIL's) in generations F_5:6 and F_5:7. The effects on grain yield and grain quality were assessed in a subpopulation of 162 RIL's chosen based on their aluminum tolerance response. Aluminum tolerance in the evaluated population was controlled by one dominant major gene with the tolerant genotypes carying Al _a Al _a and the sensitive ones al _a al _a alleles. No detrimental effects of the Al _a allele on grain yield or grain quality were detected.Part of the Master of Science dissertation of the first author     

Time-resolved spectral studies of blue-green fluorescence of artichoke (Cynara cardunculus L. Var. Scolymus) leaves: identification of chlorogenic acid as one of the major fluorophores and age-mediated changes

Synchrotron radiation and the time-correlated single-photon counting technique were used to investigate the spectral and time-resolved characteristics of blue-green fluorescence (BGF) of artichoke leaves. Leaves emitted BGF under ultraviolet (UV) excitation; the abaxial side was much more fluorescent than the adaxial side, and in both cases, the youngest leaves were much more fluorescent than the oldest ones. The BGF of artichoke leaves was dominated by the presence of hydroxycinnamic acids. A decrease in the percentage of BGF attributable to the very short kinetic component (from 42 to 20%), in the shape of the BGF excitation spectra, and chlorogenic acid concentrations indicate that there is a loss of hydroxycinnamic acid with leaf age. Studies on excitation, emission, and synchronized fluorescence spectra of leaves and trichomes and chlorogenic acid contents indicate that chlorogenic acid is one of the main blue-green fluorophores in artichoke leaves. Results of the present study indicate that 20-42% (i.e., the very short kinetic component) of the overall BGF is emitted by chlorogenic acid. Time-resolved BGF measurements could be a means to extract information on chlorogenic acid fluorescence from the overall leaf BGF.     

大豆根构型在玉米/大豆间作系统中的营养作用

 运用根构型不同的大豆品种与玉米进行间作，比较大豆根构型在玉米/大豆间作系统中的营养作用。结果表明，玉米与大豆间作具有明显的间作优势，间作作物的生物量、氮磷含量都显著好于单作。玉米与浅根型大豆品种巴西10号间作，间作优势大于与深根型大豆品种本地2号。说明大豆根构型在玉米/大豆间作系统中具有十分重要的作用。间作系统的氮磷养分竞争比率表明，玉米/大豆这类豆科/禾本科间作组合的优势主要来自对氮的优势性吸收，而玉米与不同基因型大豆间作的优势差异则主要来源于对土壤磷吸收的差异。浅根型大豆品种不仅有利于两种间作作物对土壤磷的吸收，同时还有利于对氮的吸收。     

Occurrence of clinical and sub-clinical mastitis in dairy herds in the West Littoral Region in Uruguay

Twenty-nine dairy farms were selected to determine the incidence of clinical mastitis, prevalence of sub-clinical mastitis and bacterial aetiology in the West Littoral Region of Uruguay. In samples taken by the owner and frozen at -20 degrees C during a week the incidence rate of clinical mastitis was determined as 1.2 cases per 100 cow-months at risk. Staphylococcus aureus was the most common isolated pathogen in 37.5% of 40 milk samples from clinical cases obtained in 1 month. No bacteria grew in the 32.5% of the total samples. A sub-sample including 1077 dairy cows from randomly selected farms was used to determine the prevalence of sub-clinical mastitis. These samples were taken on one visit to each farm. The prevalence was 52.4% on a cow basis and 26.7% on an udder quarter basis. In 55.1% of the quarters of the selected animals with more than 300,000 cells/ml there was no growth. The isolated pathogens from sub-clinical cases and their relative frequencies were: Staphylococcus aureus 62.8%, Streptococcus agalactiae 11.3%, Enterococcus sp. 8%, coagulase-negative staphylococci 7.4%, Streptococus uberis 6.4%, Streptococcus dysgalactiae 1.8%, Escherichia coli 1.5% and Staphylococcus hyicus coagulase-positive 0.6%.     

Cell types and their immunological functions in bovine mammary tissues and secretions--a review of the literature

In this work the literature concerning cells related to bovine mammary gland defence mechanisms has been reviewed. The cells considered in this review include leucocytes from mammary secretions, leucocytes located in the mammary tissues, and nonsecretory epithelial cells which line the teat and the lactiferous sinuses of the udder. The mammary secretions and tissues basically contain three types of cells: polymorphonuclear leucocytes, macrophages, and lymphocytes. The number of each type of cell varies, depending on the physiological and pathological states of the udder, and all cell functions are associated with the immuno-defence mechanisms of the mammary gland.     

Autologous Platelet Concentrates as an Adjunctive Treatment for Chronic Laminitis in a Mare with Pituitary Pars Intermedia Dysfunction

The aim of this article was to describe a case of a 7-year-old mare with chronic laminitis secondary to pituitary pars intermedia dysfunction that was successfully treated with autologous platelet concentrates (APC) obtained by the tube method, 4 months before being treated with oral pergolide. After the diagnosis of pituitary pars intermedia dysfunction, the patient was treated with three intralesional injections of APC at 10-day intervals. APC presented a mean concentration of 430 ± 22 × 10⁶ platelets and 8.6 ± 1.3 leukocytes/mL. The treatment with APC produced growth of the hooves at a rate of 5 mm/wk and substantially reduced the foot pain (lameness) after two APC applications. The mare could be ridden 2 weeks after the third APC treatment. The treatment with oral pergolide 4 months later improved the clinical picture of the patient, albeit hirsutism remained. APC could be useful as adjunctive treatment of chronic laminitis in horses. However, additional clinical studies are necessary to determine the safety and effectiveness of APC in chronic laminitis.     

西班牙FERTIUM MAXIMA:致力打造全球种肥领导企业

<正>Fertium总部位于西班牙萨拉戈萨市,隶属于Tervalis集团,是一家专业化肥生产商和服务分公司,为种植者提供作物营养方面最先进的技术产品,注重农业的可持续发展、尊重环境,提倡合理使用农业营养产品,责任是既满足当下农业需求,而不损害未来的可能性。     

Grass greenness flush can influence breeding phenology and fertility in equatorial thoroughbred mares in the absence of photoperiod variation

Reproductive phenology is an important trait subjected to natural selection. Current horses in America belong to the Palearctic original populations after being introduced by European colonizers. Photoperiod variation is the main environmental factor for the adjustment of reproductive timing in horses, but is absent in equatorial areas. Here we hypothesize that seasonality of green‐grass availability may influence breeding phenology in equatorial regions. We used data of 929 services to mares from 2006 to 2011 in a thoroughbred equine exploitation in Ecuador that experienced strong grass seasonality. Actual births could not be used to infer natural phenology because they were influenced by management decisions. Instead, we used variations in the probability of pregnancy after a service as a measure of the natural tendency of mares to show breeding phenology. We found that although managers tended to schedule pregnancies in two periods within the year, mares were more prone to become pregnant after the increase in grass greenness that takes place at the beginning of the year (February). Our finding has potential applications to improve the success of services and the welfare of animals, by providing green‐grass stimuli in the appropriate season.