THORACIC METASTASES OF CANINE MAMMARY GLAND TUMORS

The absence or presence of metastases on thoracic radiographs in 55 female dogs with mammary gland tumors was assessed by comparing the results of a single radiographic examination, using dorsoventral and left lateral views, with clinical and histologic follow-up data. Radiographic abnormalities found in dogs with thoracic metastases were classified as well-defined pulmonary nodules, ill-defined pulmonary nodules, and involvement of pleural effusion. No obvious difference in the effect on the right or left lung lobes was found. The sensitivity, specificity and accuracy for the detection of thoracic metastases were 65%, 97%, and 87%, respectively.     

Equine foetal gender determination in mid‐ to late gestational mares: A practical inquiry

In recent years, the interest in equine foetal gender determination (FGD) during gestation increased remarkably. Ultrasonographic FGD can be performed in two different periods during gestation. The earliest examination can take place at a gestational age of 60–70 days, whereby the genital tubercle is used to differentiate between male and female foeti. The time window of the second approach is wider (120–210 days), and there are more characteristics to take into consideration. In this article, the feasibility and accuracy of ultrasonographic FGD in mid‐ to late gestation are evaluated. One hundred twenty‐one mares from different breeds with a pregnancy stage between 120 and 270 days were examined once, using B‐scale ultrasonography (Esaote MyLab™ClassC). None of the mares were sedated nor shaved, and the procedure was completed within 15 min. Diagnosis was firstly based on the gonads. The final judgement was made based on all visible foetal reproductive organs. In three cases with a pregnancy stage beyond 257 days, FGD was not possible. All of the examined mares in which a FGD could be performed gave birth to a healthy foal. In 98% of the examinations (116/118), the diagnosis was correctly made. In both cases of misdiagnosis, only one characteristic was seen during the procedure and wrongly interpreted. Beyond 210 days of pregnancy, the extremities can preclude a good visualization of the inguinal region. In conclusion, equine FGD in mid‐ to late gestation is an accessible and accurate technique, although a good ultrasound device is a prerequisite and experience and expertise is necessary.     

Evaluation of functional muscle anatomy scalability in the canine hind limb

In contrast to other mammals, the large variation in dog sizes is not accompanied by any significant genetic re-organization. In order to study the relationship between body mass, limb length and the functional anatomical muscle parameters of the canine hind limb, a large dataset comprising of muscle masses, optimal muscle fibre lengths and physiological cross-sectional area's (PCSA) were acquired for twenty-five muscles in ten dogs of sizes varying between 20 kg and 52 kg. The potential of body mass and limb length for reliably scaling individual muscle masses, optimal muscle fibre lengths and PCSA's were examined. For the majority of the muscles of the canine hind limb, neither body mass nor limb length were reliable scaling parameter for either muscle masses, PCSA's and optimal fibre length. These results indicate the need of a breed-specific approach to musculoskeletal modelling in future canine musculoskeletal research.     

Polyuria and Polydipsia and Disturbed Vasopressin Release in 2 Dogs With Secondary Polycythemia

In dogs, secondary polycythemia (SP) may be associated with polyuria and polydipsia (PU/PD). The pathogenesis of this PU/PD has not yet been explained. We hypothesized that hyperviscosity and increased blood volume in SP might affect vasopressin (VP) release, resulting in PU/PD. This hypothesis was tested in 2 dogs with SP caused by renal neo-plasia and PU/PD. Osmoregulation of VP release was studied by a modified water deprivation test and by investigating the VP response to hypertonic saline infusion.
Water deprivation test results were consistent with an inability to produce concentrated urine despite increasing plasma osmolality. During hypertonic saline infusion, the osmotic threshold of VP release was markedly increased in both dogs, resulting in a delayed VP response to increasing plasma osmolality. The sensitivity of VP release was low normal in both dogs. We conclude that blood hyperviscosity and increased blood volume led to impaired VP release and polyuria.     

Biological control of Rhizoctonia root rot on bean by phenazine- and cyclic lipopeptide-producing Pseudomonas CMR12a

Pseudomonas CMR12a was previously selected as an efficient biocontrol strain producing phenazines and cyclic lipopeptides (CLPs). In this study, biocontrol capacity of Pseudomonas CMR12a against Rhizoctonia root rot of bean and the involvement of phenazines and CLPs in this ability were tested. Two different anastomosis groups (AGs) of Rhizoctonia solani, the intermediately aggressive AG 2-2 and the highly aggressive AG 4 HGI, were included in growth-chamber experiments with bean plants. The wild-type strain CMR12a dramatically reduced disease severity caused by both R. solani AGs. A CLP-deficient and a phenazine-deficient mutant of CMR12a still protected bean plants, albeit to a lesser extent compared with the wild type. Two mutants deficient in both phenazine and CLP production completely lost their biocontrol activity. Disease-suppressive capacity of CMR12a decreased after washing bacteria before application to soil and thereby removing metabolites produced during growth on plate. In addition, microscopic observations revealed pronounced branching of hyphal tips of both R. solani AGs in the presence of CMR12a. More branched and denser mycelium was also observed for the phenazine-deficient mutant; however, neither the CLP-deficient mutant nor the mutants deficient in both CLPs and phenazines influenced hyphal growth. Together, results demonstrate the involvement of phenazines and CLPs during Pseudomonas CMR12a-mediated biocontrol of Rhizoctonia root rot of bean.     

Resistenzigenschaften im Gersten- und Weizensortiment Gatersleben 6. Prüfung von Gersten auf ihr Verhalten gegen Gelbrost(Puccinia striiformis West. syn.P. glumarum (Schm.) Erikss. et Henn.)

Zusammenfassung Von 380 Winter- und 1400 Sommergersten des Sortiments Gatersleben wurde in Halle das Verhalten gegen die virulente Rasse 24 vonPuccinia striiformis West, untersucht. Als hochresistent erwiesen sich 5 Winter- und 43 Sommerformen. Ein großer Teil dieser Sommergersten, darunter auch wertvolle Kombinationen von Gelbrost- mit Zwergrost- bzw. Mehltauresistenz, stammen aus Aethiopien. Resistenz zeigten weitere 88 Proben oder ca. 5% des Sortiments. In 12% lagen Gemische anfälliger mit resistenten Pflanzen vor. 80% der Gersten waren mäßig bis stark anfällig für Gelbrost.

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Summary Stripe rust reaction of 380 winter and 1400 spring barley varieties of the Gatersleben collection was tested in the greenhouse as well as in the disease nursery by artificial infection with the virulent race 24 ofPuccinia striiformis West. Five winter and 43 spring barley varieties have proved to be highly resistant. A large number of the latter were collected in Ethiopia, among them are useful combinations of stripe rust with dwarf rust resistance and mildew resistance, respectively. Resistance was shown by other 88 varieties or by about 5% of the total. Susceptible as well as resistant plants were found in 12% of the samples. 80% of the varieties were moderately or highly susceptible to stripe rust.

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am ¶rt;au 380 1400 24 . 5 43 . ¶rt; , , . 88 5% . 12% . 80% .

     

Veränderungen der Muskelfasertypenverteilung im M. longissimus dorsi von Ebern während des Wachstums, dargestellt mit verschiedenen histochemischen Methoden

Changes in the distribution of muscle-fiber-types from the M. longissimus dorsi of male pigs. A Histochemical Approach Samples of the M. longissimus dorsi from growing up pigs were taken by a shooting-biopsy. The types of muscle-fibers could be demonstrated by two different histochemical staining procedures. With these two histochemical staining procedures significant differences between the different structure of the muscle-fibers during increasing age of the pigs could be detected.