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ABSTRACT: Three diets supplemented with taurine, β-alanine andGABA and a control diet were fed to juvenile and fingerling Japaneseflounder to investigate the effects of the diets on growth and metabolicchanges of free amino acids in whole body and tissues. In experimentI, three diets supplemented with 1% each of taurine, β-alanineand GABA and a control diet were fed to juvenile Japanese flounderwith an initial mean body weight of 0.4 g for 4 weeks at20°C. In experiment II, the taurine-supplemented diet anda control diet were fed to fingerling Japanese flounder with an initialmean body weight of 15 g for 4 weeks at 20°C.Only supplementation of taurine in the diet of juvenile flounderimproved their growth performance in experiment I, but fingerlinggrowth performance of experiment II was not significantly relatedto taurine supplementation in the experimental diet. These resultssuggest that there is a greater requirement for taurine for thegrowth of juvenile Japanese flounder than fingerling Japanese flounder. 相似文献
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Ikue Murata Sigefumi Saruki Katsuyoshi Kubota Sachiko Inoue Naoaki Tashiro Tsutomu Enoki Yasuhiro Utsumi Susumu Inoue 《Journal of Forest Research》2009,14(5):296-301
Effects of sika deer (Cervus nippon) and dwarf bamboo (“sasa;” Sasamorpha borealis) on seedling emergence and survival were investigated in cool-temperate mixed forests in the Kyushu mountain range, Japan.
We compared the effects of sika deer between two sites with different sika deer densities. One site (no-sasa forest) has sparse
cover of S. borealis and a high density of sika deer, and the other site (sasa forest) has dense cover of S. borealis with a low density of sika deer. In the no-sasa forest, more seedlings emerged and the survival rate of the seedlings was
higher compared with the sasa forest. Compared with the sasa forest, the canopy in the no-sasa forest was more open, the organic
layer was shallower and drier, the mean daily soil temperature was higher, and soil temperature fluctuated more widely. Those
environmental parameters did not differ between unfenced and fenced plots. The comparison between the sasa and no-sasa forests
suggested that removal of S. borealis by sika deer had an indirect positive effect on seedling emergence and survival. However, the fenced-plot experiments indicated
that sika deer inhibited seedling emergence and survival. These contradictory conclusions could result from the much larger
negative effects of S. borealis on seedling emergence and survival compared with the effects of sika deer browsing. 相似文献
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Taiki KIDA Arisa YAMAZAKI Koji KOBAYASHI Tatsuro NAKAMURA Takayuki NAKAGAWA Ryohei NISHIMURA Takahisa MURATA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(5):644
Polyunsaturated fatty acids, including arachidonic acid (AA), docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), are converted to hundreds of lipid mediators by cyclooxygenases (COX), lipoxygenases (LOX), and cytochrome P450 (CYP), or through non-enzymatic processes, and they reflect inflammatory states of the body. We comprehensively analyzed lipid metabolites in dog urine using a liquid chromatograph-mass spectrometry (LC-MS/MS) to describe their metabolic characteristics. We detected 31 AA-derived metabolites, four EPA-derived metabolites, and a DHA-derived metabolite in all urine samples. Among AA-derived metabolites, 15, 5, 3, and 8 were generated by COX, LOX, CYP, and non-enzymatic oxidation respectively. This study will be the first step to use profiles of urinary lipid metabolites for better understanding and diagnosis of canine diseases. 相似文献
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Ontogeny of digestive and immune system organs of larval and juvenile kelp grouper Epinephelus bruneus reared in the laboratory 总被引:3,自引:0,他引:3
Keitaro KATO Katsuya ISHIMARU Yoshifumi SAWADA Junichi MUTSURO Shigeru MIYASHITA Osamu MURATA Hidemi KUMAI 《Fisheries Science》2004,70(6):1061-1069
ABSTRACT: A histological examination was made of the ontogenetic development of the digestive and immune systems of the larval and juvenile kelp grouper Epinephelus bruneus reared in the laboratory. The liver, gall bladder, pancreas and the demarcating region between the intestines and rectum were formed within 3 days post-hatch (dph). During the preflexion phase (within 16 dph), revolution of the intestine concluded, and pharyngeal teeth and the mucous cells of the esophagus were differentiated. In the transitional period to the juvenile stage (25 dph), the blind sac of the stomach, gastric glands and pyloric caeca began to form. From the viewpoint of the differentiation phase of the adult-type digestive system, the kelp grouper is similar to Heterosomata, hitherto reported. The primordial thymus, kidney and spleen were present at 12, 1 and 6 dph, and the small lymphocytes in these lymphoid organs appeared at 21, 30 and 33 dph, respectively. The developmental sequence of the lymphoid organs and the appearance ages of the lymphoid organs and small lymphocytes in the lymphoid organs in the kelp grouper are similar to those of other marine fish previously reported, except for the Pacific bluefin tuna Thunnus orientalis . 相似文献
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Satoshi NOZAWA Hitomi ODA Ran AKIYAMA Kaori UEDA Kaori SAEKI Saori SHONO Natsuki MARUYAMA Atsuki MURATA Hiroyuki TAZAKI Akihiro MORI Yutaka MOMOTA Daigo AZAKAMI Toshinori SAKO Katsumi ISHIOKA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(8):1177-1182
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Kaori MURATA Miho NAGASAWA Tatsushi ONAKA Ken-ichi TAKEYAMA Takefumi KIKUSUI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(3):478
The biological and psychological significance of oxytocin is increasingly recognized; however, reliable assays of oxytocin in biological samples have not been developed. We raised a new oxytocin polyclonal rabbit antibody against synthetic oxytocin. The affinity of antibodies to oxytocin was examined by a radio-immunoassay and compared with that of a previously validated antibody. One antibody showed affinity for oxytocin in the radio-immunoassay. We developed a solid-phase ELISA for oxytocin using this antibody and compared it with existing methods. The newly developed ELISA showed comparable results using urine samples but not using serum samples. These results indicate that the new ELISA is useful for urinary oxytocin; further modifications, such as different extraction methods, are needed for its application to serum oxytocin. 相似文献