全文获取类型
收费全文 | 439篇 |
免费 | 32篇 |
专业分类
林业 | 38篇 |
农学 | 24篇 |
43篇 | |
综合类 | 18篇 |
农作物 | 11篇 |
水产渔业 | 96篇 |
畜牧兽医 | 204篇 |
园艺 | 4篇 |
植物保护 | 33篇 |
出版年
2022年 | 2篇 |
2021年 | 15篇 |
2020年 | 4篇 |
2019年 | 7篇 |
2018年 | 13篇 |
2017年 | 16篇 |
2016年 | 21篇 |
2015年 | 14篇 |
2014年 | 11篇 |
2013年 | 27篇 |
2012年 | 18篇 |
2011年 | 30篇 |
2010年 | 8篇 |
2009年 | 18篇 |
2008年 | 33篇 |
2007年 | 19篇 |
2006年 | 17篇 |
2005年 | 26篇 |
2004年 | 25篇 |
2003年 | 18篇 |
2002年 | 18篇 |
2001年 | 21篇 |
2000年 | 15篇 |
1999年 | 15篇 |
1998年 | 3篇 |
1997年 | 8篇 |
1996年 | 2篇 |
1992年 | 6篇 |
1990年 | 3篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1981年 | 2篇 |
1980年 | 1篇 |
1979年 | 5篇 |
1978年 | 2篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1970年 | 2篇 |
1969年 | 1篇 |
1968年 | 1篇 |
1967年 | 2篇 |
1966年 | 1篇 |
1965年 | 2篇 |
排序方式: 共有471条查询结果,搜索用时 15 毫秒
1.
2.
3.
Seiju Ishikawa 《Journal of General Plant Pathology》2004,70(5):249-255
Simple diagnosis by ethanol immersion (SDEI) to detect Glomerella cingulata was used to detect three other fungi that also cause latent infection of strawberry plants. Signs on strawberry leaves with asymptomatic latent infection by Colletotrichum acutatum became visible using SDEI. Salmon-pink conidial masses were produced in the acervuli on the treated leaves 5 days after incubation at 28°C. In the case of Dendrophoma obscurans, pycnidia with amber conidial masses formed 5 days after incubation at 28°C. The pycnidia were observed mainly on the ribs, and conidial masses exuded from the ostiole. These macroscopic conidial masses were similar to those of G. cingulata and C. acutatum. When water was dripped onto a lesion caused by D. obscurans, the pycnidia exuded white filamentous conidial masses, making the distinction of D. obscurans from G. cingulata or C. acutatum. On petioles with latent infection by Fusarium oxysporum f. sp. fragariae, white aerial hyphae grew out from the vascular tissues on the cut surface 3 days after incubation at 28°C and were easily observed by eye or with a loupe. Thus, SDEI was also useful for diagnosing latent infection of strawberry plants by C. acutatum, D. obscurans, and F. oxysporum f. sp. fragariae. 相似文献
4.
5.
Hiroto Miura Takuya Hashimoto Yukiko Kawanishi Hiroki Kawauchi Ryo Inoue Noriaki Shoji Kunihiko Saito Mario Sekiya Yosuke Saito Jumpei Yasuda Chiemi Yonezawa Tetsushiro Endo Hirotaka Kasuya Yutaka Suzuki Yasuo Kobayashi Satoshi Koike 《Animal Science Journal》2021,92(1):e13601
The rumen microbiota comprises a vast range of bacterial taxa, which may affect the production of high-quality meat in Japanese Black cattle. The aim of this study was to identify core rumen microbiota in rumen fluid samples collected from 74 Japanese Black cattle raised under different dietary conditions using 16S rRNA gene amplicon sequencing. In the rumen of fattening Japanese Black cattle, 10 bacterial taxa, showing >1% average relative abundance and >95% prevalence, irrespective of the dietary conditions and the fattening periods, were identified as the core rumen bacterial taxa, which accounted for approximately 80% of the rumen microbiota in Japanese Black cattle. Additionally, population dynamics of the core rumen bacterial taxa revealed two distinct patterns: Prevotella spp. and unclassified Bacteroidales decreased in the mid-fattening period, whereas unclassified Clostridiales, unclassified Ruminococcaceae, Ruminococcus spp., and unclassified Christensenellaceae increased during the same period. Therefore, the present study reports the wide distribution of the core rumen bacterial taxa in Japanese Black cattle, and the complementary nature of the population dynamics of these core taxa, which may ensure stable rumen fermentation during the fattening period. 相似文献
6.
7.
Murase T Imaeda N Yamada H Miyazawa K 《The Journal of reproduction and development》2007,53(4):853-865
This study attempted to explain the mechanisms regulating boar fertility by examining seasonal changes in semen characteristics, the composition of seminal plasma and responsiveness of sperm acrosomes to Ca(2+) and the Ca(2+) ionophore A23187 (Ca(2+)/A23187). Sperm-rich and sperm-poor fractions were separately collected from 3 mature fertile Large White boars once a month over a one-year period. During the period of study, ambient temperature and relative humidity were recorded for within the stall in which the boars were kept and the semen characteristics, composition of the seminal plasma of sperm-rich fractions, and occurrence of the acrosome reaction in response to Ca(2+) (3 mM)/A23187 (0.3 microM) were examined. The highest mean maximum and minimum ambient temperatures were recorded in August-September, whereas the lowest mean maximum and minimum ambient temperatures were recorded in December and January, respectively. There was a moderate peak in relative humidity from July to October. The lowest percentages of motile spermatozoa and of spermatozoa with intact acrosomes and highest percentage of spermatozoa with abnormal morphology and strongest agglutination were seen in August-September. The total protein and albumin concentrations were lowest in August-September. Testosterone levels increased gradually as day length decreased after the summer solstice (June) and peaked in October-November. The percentage of acrosome reactions in response to Ca(2+)/A23187 was highest with the quickest response in August-September, as shown by the shortest time required for 50% of relative acrosome reactions. The farrowing rates were lowest in these same 2 months. These results suggest that seasonal infertility in Large White boars may be due, at least in part, to a combination of low motility, abnormal morphology including acrosomal abnormality, and early occurrence of the acrosome reaction in response to stimulus, possibly resulting from a decrease in acrosomal stabilizing proteins in the seminal plasma during summer. These changes may be modulated by heat/humidity stress and/or photoperiod-regulated testosterone. 相似文献
8.
Tongjun Ren Shunsuke Koshio Orhan Uyan Connie Fay Komilus Saichiro Yokoyama Manabu Ishikawa Md Kadel Abdul 《Journal of the World Aquaculture Society》2008,39(6):797-803
A trial was conducted to determine the effect of ascorbyl‐2‐monophosphate Na/Ca (AMP‐Na/Ca) on blood chemistry and nonspecific immune response of red sea bream juveniles. Test diets with three levels of AsA (free, 107, and 325 mg/kg diet) were fed to juvenile red sea bream (36.0 ± 1.3 g) two times a day for 3 wk. There were no significant differences in hematocrit, glucose, and blood urea nitrogen. Total cholesterol and triglyceride in plasma of fish fed AsA‐free diet was significantly (P < 0.05) higher than that of fish fed two other diets. There were no significant differences in serum albumin, total bilirubin, and total serum protein. Glutamyl oxaloacetic transaminase in serum of fish fed diets containing 107 and 325 mg of AsA were significantly (P < 0.05) lower than that of fish fed AsA‐free diet. Serum lysozyme activity (LA) of fish fed diets containing 107 and 325 mg of AsA were significantly (P < 0.05) higher than that of fish fed AsA‐free diet. There was no significant difference in mucus LA. The results mentioned above demonstrated that AMP‐Na/Ca is a bioavailable AsA source for red sea bream juveniles. Supplement of more than 107 mg AsA/kg in diets improved blood chemistry and nonspecific immune function of red sea bream juveniles. 相似文献
9.
M Yamamoto M Ishikawa T Masui H Nakazawa Y Kabasawa 《Journal of the Association of Official Analytical Chemists》1985,68(3):493-494
A simple and rapid liquid chromatographic method is described for the determination of barbaloin (aloin, 10-D-glucopyranosyl-1,8-dihydroxy-3-(hydroxymethyl)-9(10H)-anthraceno ne) in foods. Barbaloin is extracted with water from foods containing aloe and the extract is cleaned up on a disposable cartridge by using methanol-water (55 + 45) as eluant. The eluted barbaloin is separated by liquid chromatography on a YMC A-302 column with methanol-water (50 + 50) mobile phase, and detected at 293 nm. Recoveries of barbaloin added to foods at the levels of 0.05 and 0.50 mg/g were 94.4-100%. Assay results for commercial food samples indicated that the present method is applicable to a variety of foods supplemented with aloe. 相似文献
10.
Ye Tian Kikuo Haibara Hiroto Toda Fangjun Ding Yanhui Liu Dongsu Choi 《Journal of Forest Research》2008,13(4):205-214
Mountain closure, considered an effective and economic measure for natural restoration of degraded forest ecosystems, has
been widely carried out in the karst region of southwest China. The aim of this study was to evaluate microbial aspects of
soil quality after mountain closure by analyzing soil microbial biomass, basal respiration, metabolic quotient, and relationships
with basic chemical properties in Guizhou Province, a karst region of the upper Yangtze River. Soil quality was considered
poor from the low levels of microbial biomass carbon (MBC), nitrogen (MBN), and microbial quotient (MBC/total C and MBN/total
N), but high metabolic quotient (qCO2). Soil pH, showing marked variation from 4.1 to 7.9 in this karst region, was proved to significantly affect soil microbial
biomass and activity. Soil microbial biomass, microbial quotient, and soil basal respiration declined significantly with decreasing
soil pH, while qCO2 showed an apparently increasing, but not statistically significant, trend. The changes in microbial biomass and activity
following the change in soil pH could possibly be because of a change in soil microbial composition, and more detailed research
is necessary. Compared with soil pH, soil organic matter content was another, more important, factor that directly restricted
microbial growth because of the serious loss as a result of disturbance. As a practical application based on microbial aspects,
introduction of some N-fixing tree species may be an active and effective measure to improve soil fertility and thus to accelerate
restoration of the forest ecosystem in the karst region. 相似文献