全文获取类型
收费全文 | 215篇 |
免费 | 18篇 |
专业分类
林业 | 20篇 |
农学 | 5篇 |
基础科学 | 1篇 |
22篇 | |
综合类 | 6篇 |
农作物 | 7篇 |
水产渔业 | 16篇 |
畜牧兽医 | 147篇 |
园艺 | 1篇 |
植物保护 | 8篇 |
出版年
2022年 | 2篇 |
2021年 | 10篇 |
2020年 | 3篇 |
2019年 | 5篇 |
2018年 | 5篇 |
2017年 | 7篇 |
2016年 | 8篇 |
2015年 | 7篇 |
2014年 | 9篇 |
2013年 | 16篇 |
2012年 | 14篇 |
2011年 | 18篇 |
2010年 | 11篇 |
2009年 | 9篇 |
2008年 | 12篇 |
2007年 | 13篇 |
2006年 | 10篇 |
2005年 | 17篇 |
2004年 | 15篇 |
2003年 | 15篇 |
2002年 | 15篇 |
2001年 | 4篇 |
2000年 | 1篇 |
1999年 | 3篇 |
1998年 | 4篇 |
排序方式: 共有233条查询结果,搜索用时 15 毫秒
1.
Treatment of cow-waste slurry by a microbial fuel cell and the properties of the treated slurry as a liquid manure 总被引:1,自引:0,他引:1
Hiroshi YOKOYAMA Hideyuki OHMORI Mitsuyoshi ISHIDA Miyoko WAKI Yasuo TANAKA 《Animal Science Journal》2006,77(6):634-638
Resource recycling and the proper treatment of animal waste to reduce its environmental impact are currently important issues for the livestock industry. A microbial fuel cell (MFC), a new type of bioreactor, is expected to play roles in both waste‐water purification and energy recovery. However, the generation of electricity from cow waste has not yet been examined. In this study, using an MFC, we examined the possibility of generating electricity from dairy‐cow waste slurry, and analyzed the properties of the treated slurry as liquid manure for resource recycling. The MFC treatment of the slurry generated electricity in a dose‐dependent manner, and the maximum power output by the MFC from a 1 g of chemical oxygen demand/L slurry was 0.34 mW/m2. After the MFC treatment, 84% of the biological oxygen demand in the slurry was removed and three essential fertilizer elements (nitrogen, phosphorus, and potassium) were retained at 84, 70, and 91% levels, respectively. The amount of ammonia nitrogen in the slurry, as an element of fast‐release fertilizer, was increased by 1.9‐fold. Although the treated slurry displayed properties that made it preferable as liquid manure, further studies to improve the electrical power output by the MFC are required for practical use. 相似文献
2.
Kosei SAKAI Tomohiro YONEZAWA Hideyuki YAMAWAKI Toshifumi OYAMADA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(10):1319-1322
Somatostatin receptor 2 (SSTR2) is a negative regulator of cell proliferation in human
breast cancer. Since there is little information about SSTR2 in canine mammary gland tumor
(MGT), we clarified its distribution and expression level in normal mammary gland, benign
MGT and malignant MGT. SSTR2 expression determined by immunohistochemical staining was
observed in the cytoplasm of luminal epithelial cells. The intensity was negatively
correlated with malignancy: normal tissues and some of the benign tumors had the highest
levels, while the malignant tumors had little or no SSTR2 expression. As for the Western
blotting, SSTR2 protein level in benign tumors was significantly lower than the normal
mammary gland. On the other hand, SSTR2 protein levels in two of three malignant tumors
were higher than the other groups. These results suggest that SSTR2 expression alters
according to the malignancy of canine MGT. 相似文献
3.
Effect of intramammary injection of rboGM-CSF on milk levels of chemiluminescence activity, somatic cell count, and Staphylococcus aureus count in Holstein cows with S. aureus subclinical mastitis 下载免费PDF全文
Hideyuki Takahashi Masaharu Odai Kenji Mitani Shigeki Inumaru Shozo Arai Rieko Horino Yuichi Yokomizo 《Canadian journal of veterinary research》2004,68(3):182-187
The effect of intramammary injection of recombinant bovine granulocyte-macrophage colony-stimulating factor (rboGM-CSF, 400 microg/10 mL) on quarter milk levels of chemiluminescence (CL) activity, and somatic cell count (SCC) and shedding pattern of Staphylococcus aureus was investigated. Ten Holstein cows, naturally infected with S. aureus were used, with either early-stage or late-stage subclinical mastitis. Injection of rboGM-CSF caused a remarkable increase in milk CL activity with a peak at 6 h after the cytokine injection in the early- and late-stage groups. In the early-stage group, milk SCC stayed around preinjection level at 6 h, rose significantly on days 1 and 2, and was followed by a smooth and significant decline to an under preinjection level (below 200 000 cells/mL) on day 7 postinjection. Alternatively, in the late-stage group, milk SCC rose significantly at 6 h after the cytokine injection and maintained high levels thereafter. The milk S. aureus count decreased drastically by the cytokine injection in the early-stage group. The bacterial count was moderately decreased in the late-stage group, but increased back to preinoculation levels on day 7 after the cytokine injection. The results suggest that the rboGM-CSF has a potential as a therapeutic agent for S. aureus infection causing subclinical mastitis of dairy cows, if the cytokine is applied at the initial stage of infection. 相似文献
4.
A role for balance of interferon-gamma and interleukin-4 production in protective immunity against Neospora caninum infection 总被引:3,自引:0,他引:3
A suitable balance in the production of Th1/Th2-type cytokines has a crucial role in the control of microbial infections. We investigated cytokine production patterns and effects during Neospora caninum infection, based on two mouse models and an in vitro system. In the acute infection of N. caninum, BALB/c-background IFN-gamma-deficient mice that were sensitive to the N. caninum infection showed high levels of IL-10 production, whereas significant levels of interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) production were observed in resistant wild type mice. BALB/c mice vaccinated with recombinant vaccinia virus expressing N. caninum surface protein NcSRS2 resisted parasite spread throughout the body, low levels of IFN-gamma production and high levels of IL-4 production were observed compared to unvaccinated animals. The treatment of N. caninum-infected cells with IFN-gamma or IL-10 decreased the host-cell viability in an in vitro system using mouse macrophage J774A.1 cells. On the other hand, IL-4, but not IL-10 administration, increased the viability of N. caninum-infected and IFN-gamma-treated cells. In the light of the balance of Th1/Th2-type cytokine production, an IFN-gamma/IL-4 balance may have a crucial role for the control of cellular responses against the parasite invasion. 相似文献
5.
Yoshinobu UEMOTO Nanae SASAGO Tsuyoshi ABE Hideki OKADA Hitomi MARUOKA Hiroaki NAKAJIMA Noriaki SHOJI Shin MARUYAMA Naohiko KOBAYASHI Hideyuki MANNEN Eiji KOBAYASHI 《Animal Science Journal》2012,83(11):719-726
Genome‐wide association mapping for complex traits in cattle populations is a powerful, but expensive, selection tool. The DNA pooling technique can potentially reduce the cost of genome‐wide association studies. However, in DNA pooling design, the additional variance generated by pooling‐specific errors must be taken into account. Therefore, this study aimed to investigate factors such as: (i) the accuracy of allele frequency estimation; (ii) the magnitude of errors in pooling construction and in the array; and (iii) the effect of the number of replicate arrays on P‐values estimated by a genome‐wide association study. Results showed that the Illumina correction method is the most effective method to correct the allele frequency estimation; pooling errors, especially array variance, should be taken into account in DNA pooling design; and the risk of a type I error can be reduced by using at least two replicate arrays. These results indicate the practical capability and cost‐effectiveness of pool‐based genome‐wide association studies using the BovineSNP50 array in a cattle population. 相似文献
6.
Naoya Yamaguchi Takashi Sayama Hiroyuki Yamazaki Tomoaki Miyoshi Masao Ishimoto Hideyuki Funatsuki 《Breeding Science》2014,64(4):300-308
Lodging tolerance (LT) is an important trait for high yield and combine-harvesting efficiency in soybean [Glycine max (L.) Merr.]. Many previous studies have investigated quantitative trait loci (QTLs) for lodging score (LS) in soybean. Most of the investigated QTLs were located in the proximal region of maturity or growth habit loci. The aim of this study was to identify genetic factors for LT not associated with maturity or growth habit. QTL analysis was performed using a recombinant inbred line (RIL) population derived from a cross between ‘Toyoharuka’ (TH), a lodging-tolerant cultivar, and ‘Toyomusume’ (TM). The genotypes of TH and TM were estimated as both e1e2E3E4 and dt1. The average LS over 4 years was used for QTL analysis, identifying a major and stable QTL, qLS19-1, on chromosome 19. The LS of the near-isogenic line (NIL) with the TH allele at Sat_099, the nearest marker to qLS19-1, was significantly lower than the NIL with the TM allele at that position. The TH allele at Sat_099 rarely had a negative influence on seed yield or other agronomic traits in both NILs and the TM-backcrossed lines. Our results suggest that marker-assisted selection for qLS19-1 is effective for improving LT in breeding programs. 相似文献
7.
Yohei Kawasaki Ryo Yamazaki Katsuyuki Katayama Tetsuya Yamada Hideyuki Funatsuki 《Plant Production Science》2013,16(4):387-397
ABSTRACTUnderstanding how maturity genes affect soybean yield formation will provide important information for crop management decisions. This study aimed to reveal how maturity genes E2 and E3 in the soybean cultivar ‘Enrei’ affect yields and yield formation in warm regions of Japan. ‘Enrei’ (e2e3) and three near-isogenic lines of ‘Enrei’ (e2E3, E2e3, and E2E3) were cultivated in 2016 and 2017 in Fukuyama, Japan (34°30′N, 133°23′E). Two sowing dates were set in each year (June sowing and July sowing). E2 extended the period from emergence to R1 and also the period from R1 to R7, whereas E3 extended only the period from emergence to R1. Interaction between E2 and E3 did not affect duration of the period from emergence to R1, but did affect the period from R1 to R7. Although seed yield did not differ between genotypes in the June sowings, the effects of E2 and E3 on seed yield in July sowing were both significant and interaction between E2 and E3 also observed. The total number of nodes increased in E3 genotypes in both sowing dates, especially in E2E3. Pod-set ratio was lower in E2 and E3 genotypes than in e2 and e3 genotypes in the June sowings, but did not differ between genotypes in the July sowings. The high yield of E2E3 genotypes in the July sowings was attributed to increased number of nodes and flower production while maintaining pod-set ratio. Appropriate choice of sowing date is suggested to be essential when using E3 genotypes.Abbreviations: HI: harvest index; NIL: near-isogenic line; RUE: radiation use efficiency; TDM: total above-ground dry matter; TRI: total solar radiation intercepted 相似文献
8.
Allele frequencies of the extension locus encoding the melanocortin-1 receptor in Japanese and Korean cattle 总被引:2,自引:0,他引:2
Shinji SASAZAKI Munehiro USUI Hideyuki MANNEN Chihiro HIURA Soichi TSUJI 《Animal Science Journal》2005,76(2):129-132
In order to estimate the influence of the Extension (E) locus in cattle coat color, the melanocortin‐1 receptor (MC1R) gene in Japanese Black, Japanese Brown and Korean (Hanwoo) cattle were sequenced. The sequences of the coding region revealed three alleles (ED, E+ and e), which were previously reported. Polymerase chain reaction‐restriction fragment length polymorphism was performed to investigate the gene frequencies of the three breeds. Japanese Black was almost composed of ED and E+ individuals, ED = 0.481 and E+ = 0.514, and no homozygous e/e, therefore that is consistent with the hypothesis that ED and E+ induce black pigment synthesis. Allele frequencies between Japanese Brown and Hanwoo were obviously different; however, recessive red e allele frequency was 0.038 for Japanese Brown and 0.948 for Hanwoo, even though both breeds have quite similar coat colors (ranging from yellowish brown to dark brown including a red coat color). This result suggested that other genes are also associated with a coat color of red and brown in cattle. 相似文献
9.
Makala LH Reyes JC Nishikawa Y Tsushima Y Xuan X Huang X Battsetseg B Matsuo T Nagasawa H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(4):491-499
The phenotype and function of peritoneal cavity macrophage-derived dendritic cells (PEC-DC) was previously reported. In this study we have gone further in using our established culture system to generated discrete Peyer's patch dendritic cells (DPP-DC) from murine discrete Peyer's patch macrophages (DPP-M?), following stimulation with granulocyte macrophage colony stimulating factor (GM-CSF) plus interleukin 4 (IL-4) for 7 days. DPP-M? from murine small intestines were obtained by mechanical disruption of discrete Peyer's patches (DPP), followed by metrizamide density gradient centrifugation to remove Peyer's patch resident DC and debri, after which an overnight adherent step in tissue culture medium was carried out for macrophage enrichment. Characterization of the generated DPP-DC was carried out using well-established criteria of morphology, expression of membrane antigens and capacity for antigen presentation. Dendritic cells expressed DEC-205, F4/80 and CD34 at high levels, but exhibited very low CD11c levels. They were shown to present soluble protein antigen to CD3(+) spleen T cells. A comparison of the surface antigen expression in the progenitor DPP-M? population and the generated DPP-DC showed a significant decrease in MHC class II levels and a marked down regulation of the co-stimulatory molecule CD86 (B7-2). High expression of the haemopoietic progenitor marker CD34 indicates that the generated DC, possess a haemopoietic rather than myeloid origin. Taken together, these results may provide a better understanding of the complex network regulating mucosal immune responses. 相似文献