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Mumma MJ DiSanti MA Magee-Sauer K Bonev BP Villanueva GL Kawakita H Dello Russo N Gibb EL Blake GA Lyke JE Campbell RD Aycock J Conrad A Hill GM 《Science (New York, N.Y.)》2005,310(5746):270-274
We quantified eight parent volatiles (H2O, C2H6, HCN, CO, CH3OH, H2CO, C2H2, and CH4) in the Jupiter-family comet Tempel 1 using high-dispersion infrared spectroscopy in the wavelength range 2.8 to 5.0 micrometers. The abundance ratio for ethane was significantly higher after impact, whereas those for methanol and hydrogen cyanide were unchanged. The abundance ratios in the ejecta are similar to those for most Oort cloud comets, but methanol and acetylene are lower in Tempel 1 by a factor of about 2. These results suggest that the volatile ices in Tempel 1 and in most Oort cloud comets originated in a common region of the protoplanetary disk. 相似文献
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Effects of ammonia and hydrogen sulfide on physical and biochemical properties of the claw horn of Holstein cows 下载免费PDF全文
Hidetoshi Higuchi Hisatoshi Kurumado Maya Mori Aiko Degawa Hideyo Fujisawa Atsutoshi Kuwano Hajime Nagahata 《Canadian journal of veterinary research》2009,73(1):15-20
The effects of ammonia and hydrogen sulfide on the physical and biochemical properties of the claw horn of Holstein cows were evaluated. Significant (P < 0.05, 0.01) decreases in hardness and elasticity were found in claw horns soaked in ammonia (NH3) and hydrogen sulfide (H2S) solutions compared with those that were soaked in water for 12, 24, and 48 h. Water absorption rate, as a indicator of permeability barrier function, increased significantly (P < 0.05) over time during the soaking period and was found to be dependent on the concentrations of NH3 and H2S in the solutions. The contents of ceramide, the main lipid component for the permeability barrier system of the stratum corneum, were significantly decreased in claw horns soaked in NH3 and H2S solutions compared with the values before soaking. Quantities of eluted protein released from claw horns treated with NH3 and H2S solutions were approximately 20 times and 30 to 40 times greater than those released from claw horns treated with water alone. Interestingly, the quantities of cytokeratin 10, the main cytoskeletal protein of the stratum corneum, eluted from claw horns treated with NH3 and H2S solutions were markedly greater than the quantity released from horns soaked in water. Our results suggest that abnormal changes in the physical property of claw horn caused by NH3 and H2S treatment are due to disruption of the biochemical property of the claw horn induced by these chemical agents derived from slurry. 相似文献
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Toshiho NISHITA Ryou ANEZAKI Kazunori MATSUNAGA Kensuke ORITO Tamae KASUYA Hideyo SAKANOUE Akiko MATSUNAGA Kazuyoshi ARISHIMA 《Journal of Equine Science》2013,24(4):57-62
Although endoscopy is the definitive diagnostic method for the detection of colonic
ulcers, the equipment required for performing the test is costly and difficult to use.
Therefore, a simple cost-effective and reliable screening test for intestinal tract
bleeding is needed. To this end, we measured carbonic anhydrase isozymes (CA-I and CA-II)
originating from erythrocytes by ELISA in order to determine if they could be used as
markers of occult blood in feces. For fecal extract preparation, 2 g of feces were mixed
with 4 ml of 0.01 M Tris-HCl (pH 8.0) containing 0.01% thimerosal. The concentrations of
CA-I and CA-II in the fecal samples of 13 clinically normal racehorses were found to be
30.0 ± 10.0 and 34.0 ± 13.0 ng/ml, respectively. Increased concentrations of CA-I were
detected in the fecal samples of 5 horses after blood administration; however, no increase
was observed in CA-II. The concentrations of CA-I and CA-II in the fecal samples of 88
racehorses with clinical signs of equine gastric ulcer syndrome (EGUS) were 115.3 ± 79.0
and 41.0 ± 42.0 ng/ml, respectively. Thus, our results indicate that CA isozymes can be
useful as markers of occult blood in the fecal samples of horses with intestinal tract
bleeding. 相似文献
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Sachi Minagawa Satoshi Sekiguchi Yuzuru Nakaso Masahiro Tomita Manabu Takahisa Hideyo Yasuda 《Journal of insect science (Online)》2015,15(1)
Silkworm has great potential as production system of recombinant mammalian proteins. When the protein products are used for medical purpose, it is required to reduce the risk of an allergy, the content of core alpha 1,3-fucosyl residue attached to the N-glycan of proteins, for example. We isolated the gene of an enzyme responsible for the transfer of core alpha 1,3-fucosyl residue, core alpha 1,3-fucosyltransferase (Fuc-T C3), from silkworm. A candidate cDNA for silkworm Fuc-T C3 was isolated as a homolog of the fruit fly enzyme gene fucTA. The gene was located on chromosome 7 of the silkworm genome and was composed of seven exons, which spanned approximately 10 kb on the genome. The coding region of the gene was 1,350 bp and encoded a 450-amino acid protein with a molecular mass of 52.2 kDa. Deduced amino acid sequence of the coding region showed one transmembrane domain in its N-terminal and typical motifs common to fucosyltransferases including Fuc-T C3s of other organisms in its C-terminal. The extract of CHO cells transfected with the cDNA showed Fuc-T C3 activity using GDP-fucose and DABS-GnGn peptide as substrates. These results showed this cDNA clone actually encodes silkworm Fuc-T C3. 相似文献
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