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2.
K. Tagata S. Yokoyama T. Ginbo M. Honda T. Okimura M. Odakura M. Nomura S. Yamamoto 《Veterinary research communications》1996,20(1):21-30
A capillary reversed passive latex agglutination test (capillary RPLA) was developed which allows quantification of serum C-reactive protein (CRP) within approximately 15 min. The logarithmic regression line (calibration curve) obtained after measuring each CRP concentration three times in twofold dilutions of a standard canine serum containing 222 g/ml of CRP was y=6.394+0.030x (r=0.995). Capillary RPLA permitted quantification of CRP in the range 6.9–222 g/ml. The coefficients of variation ranged from 10.28% to 12.40%. The recovery rates (percentage recovery) of CRP by capillary RPLA were within the range 87% to 106%. On measuring the CRP concentrations in sera from 78 dogs by capillary RPLA, single radial immunodiffusion (SRID) and enzyme-linked immunosorbent assay (ELISA), close correlations were demonstrated between SRID and capillary RPLA (y=7.250+1.109x, r=0.978), between SRID and ELISA (y=3.042+1.059x, r=0.967), and between capillary RPLA and ELISA (y=1.778+0.929x, r=0.962). Capillary RPLA may be considered useful as a routine biochemical technique for measurement of serum CRP concentration in the dog.Abbreviations CRP
C-reactive protein
- ELISA
enzyme-linked immunosorbent assay
- RPLA
reversed passive latex agglutination test
- SRID
single radial immunodiffusion 相似文献
3.
Within a forested watershed at the Uryu Experimental Forest of Hokkaido University in northern Hokkaido, overstory litterfall and related nutrient fluxes were measured at different landscape zones over two years. The wetland zone covered with Picea glehnii pure stand. The riparian zone was deciduous broad-leaved stand dominated by Alnus hirsuta and Salix spp., while the mixture of deciduous broadleaf and evergreen conifer dominated by Betula platyphylla, Quercus crispula and Abies sachalinensis distributed on the upland zone. Annual litterfall averaged 1444, 5122, and 4123 kg.hm^-2·a^-1 in the wetland, riparian and upland zones, respectively. Litterfall production peaked in September-October, and foliage litter contributed the greatest amount (73.4%-87.6 %) of the annual total litterfall. Concentrations of nutrients analyzed in foliage litter of the dominant species showed a similar seasonal variation over the year except for N in P glehnii and A. hirsuta. The nutrient fluxes for all elements analyzed were greatest on riparian zone and lowest in wetland zone. Nutrient fluxes via litterfall followed the decreasing sequence: N (11-129 kg.hm-2.aq) 〉 Ca (9-69) 〉 K (5-20) 〉 Mg (3-15) 〉 P (0.4-4.7) for all stands. Significant differences were found in litterfall production and nutrient fluxes among the different landscape components. There existed significant differences in soil chemistry between the different landscape zones. The consistently low soil C:N ratios at the riparian zone might be due to the higher-quality litter inputs (largely N-fixing alder). 相似文献
4.
Yamana H Ito H Ito T Murase T Motoike K Wakabayashi K Otsuki K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(2):217-219
Heated and hydrated naturally occurring dolomite showed very strong antiviral activity. Infectivity of avian and human influenza, avian infectious bronchitis (coronavirus), Newcastle disease (paramyxovirus) and avian laryngotracheitis (herpesvirus) viruses dropped at least 1,000 fold following contact with the dolomite for five minutes at 4 degrees C. Dolomite is expected to be useful to inhibit the incidence of emerging and re-emerging infectious diseases. 相似文献
5.
Two new species of myxozoans from the Japanese anglerfish, Lophius litulon, are described using myxospore morphology and small subunit rDNA sequences. Ceratomyxa anko sp. n. is a parasite of the gall bladder and had a prevalence of 57%. Mature spores of C. anko sp. n. are arcuate to crescent shaped with valves tapering to rounded tips. A prominent sutural line runs centrally between the round adjacent polar capsules containing the polar filament coiled two to three times. Spore measurements: length 10.8 (9.7-11.9) microm, width 41.9 (36.9-47.2) microm, polar capsule diameter 4.6 (4.1-5.3) microm. Ceratomyxa anko sp. n. can be distinguished from other Ceratomyxa spp. due to its spore dimensions and shape. Zschokkella lophii sp. n. is a parasite of the urinary bladder and had a prevalence of 70%. Mature spores are ellipsoidal to semicircular with bluntly pointed ends. The sutural line is curved or sinuous and the valves have no discernable surface ornamentation. Two almost spherical polar capsules are located separately in the ends of the spore, opening in almost opposite directions and contain the polar filament with five coils. Spore measurements: length 20.1 (16.8-24.0) microm, width 14.9 (12.7-16.8) microm, polar capsule diameter 5.1 (3.6-5.8) microm. Zschokkella lophii sp. n. can be distinguished from other Zschokkella spp. due to the terminal opening of the polar capsules within the spores and the site of infection within the host fish. In the phylogenetic analyses, C. anko sp. n. grouped with other members of the same genus forming a monophyly. Zschokkella lophii sp. n. forms a discrete clade with another Zschokkella sp. that infects the urinary bladder of marine fish. This grouping forms a sister clade to one containing members of the genus Parvicapsula, all of which are parasites of the urinary system in marine fish. 相似文献
6.
Md. Abdul Kader Shunsuke Koshio Manabu Ishikawa Saichiro Yokoyama Mahbuba Bulbul Binh Thanh Nguyen Jian Gao Asda Laining 《Aquaculture Research》2012,43(10):1427-1438
An experiment was carried out to evaluate fermented soybean meal and squid by‐product blend (1:1) (FP) as replacement of fishmeal (FM) for Japanese flounder, Paralichthys olivaceus. Five isocaloric (19 kJ g?1) diets were prepared by replacing 0 (FP0), 12 (FP12), 24 (FP24), 36 (FP36) and 48% (FP48) FM protein with FP. Triplicate groups of juveniles (mean weight of 3.9 g) were delivered the test diets for 8 weeks in a flow‐through sea water system. The results showed that there were no significant differences (P > 0.05) among the growth rates of fish fed FP0, FP12, FP24 and FP36 diets. Growth and nutrient utilization parameters were significantly reduced in fish fed FP48 diet. Although, whole body proximate composition of fish were not significantly affected by the dietary treatments compared to the control; methionine and phenylalanine contents were significantly decreased in FP48 group. Protein retention was also significantly decreased in the similar group of fish. Dietary treatments did not alter most of the plasma metabolites, while some of the health parameters were improved in the replacement groups. Results suggested that FP is a potential candidate for alternative protein ingredient in aquafeed and can replace 36% FM protein in the diet of Japanese flounder. 相似文献
7.
Passive protection of dogs against clinical disease due to Canine parvovirus-2 by specific antibody from chicken egg yolk 总被引:4,自引:0,他引:4 下载免费PDF全文
Sa Van Nguyen Kouji Umeda Hideaki Yokoyama Yukinobu Tohya Yoshikatsu Kodama 《Canadian journal of veterinary research》2006,70(1):62-64
The protective effect of immunoglobulins derived from chicken egg yolk (IgY) against infection by Canine parvovirus 2 (CPV-2) was evaluated in 10 beagle dogs orally challenged with a strain of the virus. The 2-mo-old dogs were divided into 3 groups and treated with powders containing CPV-2 IgY or normal egg yolk for 7 d after the challenge. The 4 dogs receiving normal egg yolk (control group) demonstrated mild symptoms typical of CPV-2 infection, such as vomiting, diarrhea, and weight loss. No symptoms were observed by 16 d after challenge in the 3 dogs receiving 2 g of IgY powder. Of the 3 dogs receiving 0.5 g of IgY powder, 2 had clinical CPV-2 disease; however, the manifestations were less severe than in the control group. Furthermore, the IgY-treated groups had significantly greater weight gain and shorter duration of virus shedding than the control group. These results indicate that IgY is useful in protecting dogs from CPV-2-induced clinical disease. 相似文献
8.
Mamoru Satou Kazufumi Nishi Masaharu Kubota Michiko Fukami Hideaki Tsuji Kees van Ettekoven 《Journal of General Plant Pathology》2006,72(3):193-194
The races for the causal agent of spinach downy mildew Peronospora farinosa f. sp. spinaciae were identified by inoculation of race-differential cultivars. One isolate was identified as Pfs:5s and the others belonged
to a new race. This is the first report of race Pfs:5 and another new race in Japan. 相似文献
9.
Koiwai M Hamaoka T Haritani M Shimizu S Zeniya Y Eto M Yokoyama R Tsutsui T Kimura K Yamane I 《Veterinary parasitology》2006,135(2):175-179
Serum samples from 2420 clinically healthy dairy cattle, randomly selected from stored sera in 18 districts of Japan, were tested for the presence of Neospora caninum antibodies using an indirect fluorescent antibody test (titer > or =1:200). Nationwide seroprevalence is estimated at 5.7% (139/2420). Seropositive cattle were detected in all surveyed districts despite the evidence of confirmed case reports of bovine neosporosis, showing that N. caninum is widely distributed throughout Japan. Age-specific seroprevalence did not increase with cattle age, suggesting that Neospora infection is likely to be transmitted vertically rather than horizontally in Japan. Considering that N. caninum seropositive cows are thought to be more likely to abort, substantial fetal losses may be induced by N. caninum infection in Japan. Devising strategies are needed to reduce the economic impact on the Japanese dairy industry. This is the first study to investigate the nationwide seroprevalence of N. caninum in cattle in Asia. 相似文献
10.
Furuuchi R Yokoyama T Watanabe Y Hirayama M 《Journal of agricultural and food chemistry》2011,59(8):3738-3746
Proanthocyanidins and other polyphenols in the seeds and juice of boysenberry were quantitatively analyzed. Polyphenolic extracts were prepared from the waste seeds and commercial juice by chromatographic fractionation. Compositional analysis revealed that both extracts contained six polyphenolic classes: flavanol monomers, proanthocyanidins, anthocyanins, ellagic acid, ellagitannins, and flavonol glycosides. Ellagitannins were the most abundant polyphenols in both extracts. Proanthocyanidins were present as short oligomers consisting of dimeric and trimeric procyanidins and propelargonidins, with the most abundant component being procyanidin B4 in both extracts. Quantification by high-performance liquid chromatography-mass spectrometry (HPLC-MS) revealed that the seeds contained a 72-fold higher amount of proanthocyanidins than the juice. These results indicate that boysenberry fruits contain short oligomeric proanthocyanidins along with flavanol monomers and the seeds represent a good source of short oligomeric proanthocyanidins. 相似文献